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Stable creatinine detection kit and use method thereof

A detection kit and kit technology, which is applied in the field of kits for measuring chemical and physical properties of materials, can solve problems such as poor stability, high detection results, and reduced detection accuracy, and achieve extended validity period, low toxicity, and The effect of maintaining stability

Inactive Publication Date: 2017-12-22
WHITMAN BIOTECH NANJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing Jaffe's picric acid method reagent has poor stability, and 2,4,6-trinitrophenol will obviously turn red within 24 hours after mixing with alkaline buffer solution, resulting in high detection results and low detection accuracy.

Method used

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  • Stable creatinine detection kit and use method thereof
  • Stable creatinine detection kit and use method thereof
  • Stable creatinine detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The stable creatinine detection kit raw material and proportioning ratio of the present embodiment are as follows:

[0061] Reagent 1 is as follows:

[0062]

[0063]

[0064] Reagent 2 is as follows:

[0065]

[0066] Wherein the HEPES buffer pH value is 7.5.

Embodiment 2

[0068] The stable creatinine detection kit raw material and proportioning ratio of the present embodiment are as follows:

[0069] Reagent 1 is as follows:

[0070]

[0071]

[0072] Reagent 2 is as follows:

[0073]

[0074] Wherein the HEPES buffer pH value is 7.5.

Embodiment 3

[0076] The stable creatinine detection kit raw material and proportioning ratio of the present embodiment are as follows:

[0077] Reagent 1 is as follows:

[0078]

[0079] Reagent 2 is as follows:

[0080]

[0081] Wherein the HEPES buffer pH value is 7.5.

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Abstract

The invention discloses a stable creatinine detection kit and a use method thereof, relates to the field of biochemical detection and aims to provide a creatinine detection kit which is low in toxicity, high in stability and sensitive and accurate in detection and a use method of the creatinine detection kit. The creatinine detection kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from an HEPES buffer, EDTA (ethylenediaminetetraacetic acid), creatinase, sarcosine oxidase, erythromycin, PEG8000, tween 20, TOOS, a compound stabilizer and a proclin preservative; the reagent 2 is prepared from the HEPES buffer, catalase, creatininase, 4-aminoantipyrine, NPEO, potassium ferricyanide, sodium chloride, ethylene glycol and other substances. The kit has good compatibility, better stability and low toxicity and can resist interference of bilirubin and metal ions.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a kit for measuring materials by means of measuring their chemical and physical properties. Background technique [0002] Creatinine (creatinine, Cr) is the product of muscle metabolism in the human body, and every 20g of muscle metabolism can produce 1mg of creatinine. Blood creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is the product of meat food metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. When the intake of meat food is stable and the body's muscle metabolism does not change significantly, the production of creatinine will be relatively constant. [0003] In muscle, creatine is mainly slowly formed into creatinine through irreversible non-enzymatic dehydration reaction, and then released into the blood and excreted with urine. Therefore, serum creatinine is closely related to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/70
CPCG01N33/70
Inventor 郑佳孙文勇常俊骏梁芬
Owner WHITMAN BIOTECH NANJING
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