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Sarcosine detection device as well as preparation method and application thereof

A detection device, sarcosine technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor comprehensive performance, low detection limit and high detection cost of sarcosine detection technology, and achieve simple structure and low detection limit , the effect of high sensitivity

Active Publication Date: 2017-05-31
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a sarcosine detection device and its preparation method and application, aiming at solving the problem of poor comprehensive performance of the existing sarcosine detection technology, high detection cost and low detection limit. Low enough, low sensitivity and low accuracy

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  • Sarcosine detection device as well as preparation method and application thereof
  • Sarcosine detection device as well as preparation method and application thereof
  • Sarcosine detection device as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of Organic Electrochemical Transistor (OECT): Stick the cleaned glass on the mask with the designed pattern, deposit metal electrodes by thermal evaporation, and deposit 10nm of Cr and 100nm of Au respectively to obtain Au / Cr / glass The electrode, on which a layer of poly(3,4-ethylenedioxythiophene)-polystyrenesulfonic acid (PEDOT:PSS) doped with dimethyl sulfoxide (DMSO) is spin-coated, will not need to cover PEDOT: The place where the PSS film is wiped clean. Annealing at 180°C for 30 minutes in a nitrogen atmosphere made the PEDOT:PSS film more firmly attached to the electrode surface and finally obtained an organic electrochemical transistor;

[0045] To make platinum electrodes modified with sarcosine oxidase: ultrasonically clean the platinum electrodes with acetone, ethanol and water for 10 minutes and dry them for use; take 20 μL of 20 mg / ml sarcosine oxidase solution and 10 μL of 1% Nafion solution Mix evenly and drop on the surface of the platinum ...

Embodiment 2

[0049] Preparation of Organic Electrochemical Transistor (OECT): Stick the cleaned glass on the mask with the designed pattern, deposit metal electrodes by thermal evaporation, and deposit 10nm of Cr and 100nm of Au respectively to obtain Au / Cr / glass The electrode, on which a layer of poly(3,4-ethylenedioxythiophene)-polystyrenesulfonic acid (PEDOT:PSS) doped with dimethyl sulfoxide (DMSO) is spin-coated, will not need to cover PEDOT: The place where the PSS film is wiped clean. Annealing at 180°C for 30 minutes in a nitrogen atmosphere made the PEDOT:PSS film more firmly attached to the electrode surface and finally obtained an OECT device;

[0050] Preparation of carbon nanotube dispersion: add 1.5 mg of multi-walled carbon nanotubes to 5 ml of PBS solution containing 1% Nafion, and ultrasonically disperse for 4 hours to obtain a uniformly dispersed multi-walled carbon nanotube solution of 0.3 mg / ml;

[0051] Preparation of grid electrodes modified with carbon nanotubes and...

Embodiment 3

[0055] Preparation of Organic Electrochemical Transistor (OECT): Stick the cleaned glass on the mask with the designed pattern, deposit metal electrodes by thermal evaporation, and deposit 10nm of Cr and 100nm of Au respectively to obtain Au / Cr / glass electrode, on which a layer of poly-3,4-ethylenedioxythiophene:polystyrenesulfonic acid aqueous solution (PEDOT:PSS) doped with dimethyl sulfoxide (DMSO) is spin-coated, will not need to cover PEDOT:PSS Wipe off the membrane area. Annealing at 180°C for 30 minutes in a nitrogen atmosphere made the PEDOT:PSS film more firmly attached to the electrode surface and finally obtained an OECT device;

[0056] Preparation of platinum nanoparticles-modified grid electrode: The platinum electrode was ultrasonically cleaned with acetone, ethanol and water for 10 minutes and dried for use; the cleaned platinum electrode was used as the working electrode, the counter electrode (platinum sheet) and the reference electrode ( Silver / silver chlor...

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Abstract

The invention discloses a sarcosine detection device as well as a preparation method and application thereof. The sarcosine detection device comprises an organic electrochemical transistor and a gate electrode with sarcosine oxidase immobilized. When the sarcosine to be detected has chemical reaction with the sarcosine oxidase on the gate electrode, the interfacial potential change of the gate electrode is caused, and finally, the detection of the sarcosine concentration is achieved by measuring the change of the channel current of the organic electrochemical transistor. The organic electrochemical transistor adopted by the invention has the functions of sensing and signal amplification, and has very high sensitivity and very low detection limit in sarcosine detection; and the sarcosine detection device provided by the invention has the advantages of simple structure, low production cost and low working voltage, and can achieve portable detection.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a sarcosine detection device and its preparation method and application. Background technique [0002] Prostate cancer (prostate cancer) is one of the most common malignant tumors of the male urogenital system, and its mortality rate ranks second among all cancers in men. In China, although the incidence of prostate cancer is lower than that of European and American countries, it has shown a rapid upward trend in recent years and has become a major problem for the health of middle-aged and elderly men in my country. Currently, in clinical practice, prostate specific antigen (PSA), as an important marker of prostate tumors, has been widely used in the screening of prostate cancer. However, PSA is only a prostate tissue-specific antigen, not a prostate tumor-specific antigen, so there are still some doubts about the accuracy of the detection process, and there is a phe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/414
CPCG01N27/4145G01N27/4146
Inventor 胡进林鹏宋家俊严锋柯善明曾燮榕
Owner SHENZHEN UNIV
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