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Test strip for detecting creatinine by electrochemical method and preparation method thereof

An electrochemical and test strip technology, applied in the detection field, can solve the problems of high price, complicated operation, inconvenience for users or patients, etc., and achieve the effect of simple use

Pending Publication Date: 2020-05-22
杭州联晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The detection of creatinine is now mainly carried out by wet chemical method, and the test kit is equipped with a large biochemical analyzer. It takes a long time and the operation is complicated, and it is performed by professionals.
cause some inconvenience to users or patients
As for the dry test strip test, there is no product in China, and ISATA’s creatinine dry film is available internationally, but due to the high price and relatively limited storage conditions, the dry film test of other manufacturers is basically inaccurate, mainly because it cannot Remove the interference of creatine in the sample, resulting in uneven test results, resulting in very few people using the dry method to detect creatinine

Method used

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  • Test strip for detecting creatinine by electrochemical method and preparation method thereof
  • Test strip for detecting creatinine by electrochemical method and preparation method thereof
  • Test strip for detecting creatinine by electrochemical method and preparation method thereof

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preparation example Construction

[0048] A preparation method of a test strip for electrochemical detection of creatinine, comprising the steps of:

[0049] Step 1: Prepare the first reaction solution according to the following formula, the formula includes: 5-100KU / L creatinase, 5-200KU / L sarcosine oxidase, 5-200KU / L creatine hydrolase, 1-100KU / L ascorbic acid Oxidase, 1-100KU / L peroxidase, 10-200g / L dispersant, 0.1-10g / L surfactant, 0.05-1M buffer; stir for one hour before use;

[0050] Step 2: Prepare the second reaction solution according to the following formula, the formula includes: 5-200KU / L sarcosine oxidase, 5-200KU / L creatine hydrolase, 1-100KU / L ascorbate oxidase, 1-100KU / L Peroxidase, 10-200g / L stabilizer, 0.1-10g / L surfactant, 0.05-1M buffer; stir for one hour before use;

[0051] Step 3: preparing the first enzyme carrier layer 321,

[0052] Soak the enzyme carrier layer in the first reaction solution for 1-10 minutes, take it out, and dry it for 10-60 minutes at 25-50 degrees to obtain the fi...

Embodiment 1

[0061] The preparation method of finished product 1 comprises the following steps:

[0062] Step 1: Prepare the first reaction solution according to the following formula, which includes: 50KU / L creatinase, 100KU / L sarcosine oxidase, 100KU / L creatine hydrolase, 20KU / L ascorbate oxidase, 50KU / L peroxidase Phosphate buffer, 20g / L polyvinylpyrrolidone, 0.1g / L Emulgen B66, 0.05MPH=6 phosphate buffer; stir for one hour before use;

[0063] Step 2: Enzyme carrier layer 321: soak the enzyme carrier layer in the reaction solution for 5 minutes, take it out, and dry it at 45 degrees for 20 minutes before use;

[0064] Step 3: Preparation of working carbon slurry: Add the electron mediator ferrocenemethanol into the carbon slurry CH-10, stir well and set aside

[0065] Step 4: Take the substrate 100, first print the counter electrodes 111 and 112, then take the above-prepared working carbon paste and print the working electrodes 101 and 102, then print the insulating layer 201, and dry...

Embodiment 2

[0073] The preparation method of finished product 2 comprises the following steps:

[0074] Step 1: Prepare the first reaction solution according to the following formula, which includes: 50KU / L creatinase, 100KU / L sarcosine oxidase, 100KU / L creatine hydrolase, 20KU / L ascorbate oxidase, 50KU / L peroxidase Phosphate buffer, 20g / L polyvinylpyrrolidone, 0.1g / L Emulgen B66, 0.05MPH=6 phosphate buffer; stir for one hour before use;

[0075] Step 2: Prepare the second reaction solution according to the following formula, the formula includes: 100KU / L sarcosine oxidase, 100KU / L creatine hydrolase, 20KU / L ascorbate oxidase, 50KU / L peroxidase, 20g / L Polyvinylpyrrolidone, 0.1g / LEmulgen B66, 0.05MPH=6 phosphate buffer; stir for one hour before use;

[0076] Step 3: the first enzyme carrier layer 321: soak the enzyme carrier layer in the first reaction solution for 5 minutes, take it out, dry it at 45 degrees for 20 minutes, and set it aside;

[0077] Step 4: the second enzyme carrier la...

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Abstract

The invention discloses a test strip for detecting creatinine by an electrochemical method. The test strip comprises: a substrate, a working electrode, a reference electrode, a counter electrode, a startup electrode, an insulating layer, a first enzyme carrier layer, a second enzyme carrier layer, a blood filtering membrane, a diffusion layer, a double faced adhesive tape layer, siphon holes and ahydrophilic layer. A formula of a first reaction solution on the first enzyme carrier layer comprises: creatininase, sarcosine oxidase, creatine hydrolase, ascorbic acid oxidase, peroxidase, a dispersing agent, a surfactant and a buffer solution; a formula of a second reaction solution on the second enzyme carrier layer comprises: sarcosine oxidase, creatine hydrolase, ascorbic acid oxidase, peroxidase, a stabilizer, a surfactant and a buffer solution; the detection result of the invention is well matched with a hospital inspection result, and the use is simple.

Description

technical field [0001] The invention relates to the detection field, in particular to a test strip for electrochemically detecting creatinine and a preparation method thereof. Background technique [0002] Creatinine (creatinine, CRE) is a product of human muscle metabolism, including exogenous and endogenous. Exogenous creatinine is the product of meat food metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. Under normal circumstances, the content of creatinine in the human body is basically stable. If the body's muscle volume does not change significantly, the production of endogenous creatinine is relatively constant, and it is mainly excreted through glomerular filtration. Therefore, in the case of stable exogenous creatinine, the concentration of creatinine in the blood can be used as one of the indicators for detecting glomerular filtration function. Generally speaking: male: 44-133 μmol / L female: 70-106 μmol / L. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/543
CPCG01N27/327G01N33/54366G01N2800/347
Inventor 陈军刚
Owner 杭州联晟生物科技有限公司
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