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Process for producing iodine from copper containing oxidases useful as iodine oxidases

A technology of copper oxidase and iodide, which is applied in the direction of oxidoreductase, biochemical equipment and methods, and microbial measurement/inspection, and can solve complex, expensive, dangerous and other problems

Inactive Publication Date: 2001-10-03
NOVO NORDISKBIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, this method has the disadvantage of requiring dangerous and volatile peroxides or peracids, which have to be transported or generated in situ by additional enzymatic or chemical steps, thus making the system more complex. complex and / or more expensive

Method used

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  • Process for producing iodine from copper containing oxidases useful as iodine oxidases
  • Process for producing iodine from copper containing oxidases useful as iodine oxidases
  • Process for producing iodine from copper containing oxidases useful as iodine oxidases

Examples

Experimental program
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Effect test

Embodiment

[0017] Embodiment I. Materials and methods

[0018] A. raw material

[0019]Chemicals used as buffers and substrates were at least reagent grade commercial products. Ascorbate oxidase (squash species), ceruloplasmin (porcine plasma), bilirubin oxidase (Myrocorroderma verruca) and tyrosinase (mushroom) were purchased from Sigma and used as received. Enzyme content in lyophilized powders or solutions was confirmed using UV-vis absorption and published extinction coefficients. The activity of ascorbate oxidase was confirmed with ascorbic acid.

[0020] B. method

[0021] Recombinantly produced Myceliophthora thermophila laccase (r-MtL; described in co-pending U.S. Serial No. 08,278,473, the contents of which are hereby incorporated by reference) was purified and treated with syringaldazine and 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid)·(ABTS) oxidation reaction to determine its activity: syringaldazine oxidation reaction was performed in a 1 cm quartz cuvette c...

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PUM

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Abstract

The present invention relates to a method for oxidation of iodide which comprises contacting, in an aqueous solution, a copper-containing oxidaze enzyme and a source of ionic iodide(I-), for a time and under conditions sufficient to permit the conversion of ionic iodide to iodine by the enzyme. The copper-containing enzymes may be, for example, a laccase or a bilirubin oxidaze.

Description

field of invention [0001] The invention relates to a method for iodide oxidation. More precisely, the invention relates to the use of copper-containing oxidases, in particular laccases, for the oxidation of iodide. Background of the invention [0002] For many years iodine (I 2 ) has been used as a disinfectant in many situations. Skin cleansers, wound disinfection, contact lens cleaning, and water hygiene are just a few of the uses for which iodine is used. In addition, iodine is also suitable for use in catalysts and as an animal feed additive. Suitable for drug neutralization and as a polymer precursor additive. Although iodine-based disinfection systems are extremely effective, several factors limit the scope of direct iodine application. In particular, the production, storage, transportation and handling of iodine are extremely hazardous, here due to the chemicals involved in production and the toxicity of iodine itself even at moderate concentrations. Typically, ...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12N9/02C12Q1/26
CPCC12Q1/26C12N9/0061
Inventor 徐丰
Owner NOVO NORDISKBIOTECH INC
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