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High-efficiency enzymatic biological fuel battery cathode and preparation method thereof

A biofuel cell and cathode technology, applied in biochemical fuel cells, battery electrodes, circuits, etc., can solve the problems of unsatisfactory demand and low current efficiency, and achieve excellent catalytic effect

Active Publication Date: 2012-07-11
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although people generally use the direct electrochemical properties of laccase to construct oxygen reduction cathodes for enzyme-based biofuel cells, the current efficiency of the prepared oxygen reduction cathodes is low, which has become a "bottleneck" that limits the output power of biofuel cells. ", unable to meet the needs of the current development of biofuel cells

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  • High-efficiency enzymatic biological fuel battery cathode and preparation method thereof
  • High-efficiency enzymatic biological fuel battery cathode and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1, the purification of laccase

[0021] Dissolve 50mg of laccase (Trametes Versicolor E.C.1.10.3.2, 23.76U / mg) in 500μL of 0.1M phosphate buffer solution (pH 6.0) and transfer to a dialysis bag, and soak the dialysis bag in fresh 0.1M phosphate buffer solution (pH6.0) for 4 hours with stirring and dialysis, then moved to another fresh 0.1M phosphate buffer solution (pH6.0) and stirred for 4 hours, and thus cycled for 1-2 days. Then add ammonium sulfate to the solution in the bag until it is saturated, and a precipitate precipitates out, and then the suspension is centrifuged at a speed of 5000 rpm for 10 minutes; the resulting precipitate is dissolved in about 500 μL of 0.1M phosphate buffer solution (pH6.0) , then saturated with ammonium sulfate, and precipitates were separated out; centrifuged again to obtain precipitates, and the resulting precipitates were dissolved in 300 μL 0.1M phosphate buffer solution (pH6.0) and put into dialysis tubes, and concentr...

Embodiment 2

[0022] Embodiment 2, the preparation of high-efficiency enzyme-type biofuel cell cathode

[0023] Continuously sonicate 2mg of carbon nanotubes (Shenzhen Nanoport Co., Ltd., diameter 2 , Ultrasonic frequency is 40kHz) 1 hour, dispersion treatment forms the suspension of 4mg per milliliter, takes 3 μ L of suspension and coats on the glassy carbon substrate electrode of 0.07 square centimeters, dries in the air, forms the carbon nanomaterial layer. Laccase needs to be purified before use, and the specific steps are as described in Example 1. Mix 100 μL of 1000 U / ml laccase (Trametes Versicolor E.C.1.10.3.2, 23.76 U / mg) solution obtained after purification with 20 μL ethanol to obtain a 100 U laccase mixture, and apply 1.5 μL of the laccase mixture on the carbon nanometer On the material layer, the enzyme layer is obtained by drying at room temperature, and the preparation of the high-efficiency enzyme-type biofuel cell cathode is completed.

[0024] The oxygen reduction perform...

Embodiment 3

[0025] Embodiment 3, the preparation of high-efficiency enzyme-type biofuel cell cathode

[0026] 1 mg of carbon nanospheres (synthesized by the Institute of Chemistry, Chinese Academy of Sciences, 0.5 μm in diameter, reference: Macromolecular Chemistry and Physics 207(18): 1633-1639) were continuously ultrasonicated in 1 mL of dimethylformamide (ultrasonic intensity was about 1w / cm 2 , Ultrasonic frequency is 40kHz) 0.1 hour, dispersion treatment forms the suspension of 1mg per milliliter, takes 3.5 μ L of suspension and is coated on the glassy carbon substrate electrode of 0.07 square centimeters, dries in air, forms the carbon nano material layer; Mix 60 μL of 1000 U / ml bilirubin oxidase (BOD, E.C.1.10.3.2, from Myrothecium verrucaria) solution with 20 μL acetone to obtain a mixture containing 60 U bilirubin oxidase, take 1.8 μL of bilirubin oxidase and mix The solution is coated on the carbon nanomaterial layer, and dried at room temperature to obtain an enzyme layer, th...

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Abstract

The invention discloses a high-efficiency enzymatic biological fuel battery cathode and a preparation method thereof. The biological fuel battery cathode comprises a substrate electrode, a carbon nano-material layer coated on the substrate electrode and an enzymatic layer coated on the carbon nano-material layer, wherein the enzymatic layer comprises the following a material a) and b): a) laccaseor bilirubin oxidase, b) the laccase or the bilirubin oxidase, which is cross-linked by a cross linking agent. Compared with an ordinary enzymatic biological fuel battery cathode, the enzymatic biological fuel battery cathode of the invention has higher oxygen catalytic reduction efficiency.

Description

technical field [0001] The invention relates to a high-efficiency enzyme-type biofuel cell cathode and a preparation method thereof. Background technique [0002] Enzyme biofuel cell is a new type of energy technology that uses enzymes as catalysts to convert the chemical energy of biological substances (such as glucose and oxygen) into electrical energy. pacemaker power supply, etc.) has a very wide range of application prospects (Yah et al., Carbon-Nanotube-Based Glucose / O 2 Biofuel Cells. Adv. Mater. 2006, 18, 2639-2643; Gao et al., An enzymatic glucose / O 2 biofuel cell: Preparation, characterization and performance in serum, Electrochem. Commun. 2007, 9, 989-996). Due to the use of enzymes as bioelectrochemical catalysts, the electrochemical properties of enzymes play a key role in the performance of enzyme-based biofuel cells. For example, when using electron mediator (Fei et al., A Biopolymer Composite that Catalyzes the Reduction of Oxygen to Water. Chem. Mater.,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01M4/96H01M4/90H01M8/16H01M4/88
CPCY02E60/527Y02E60/50
Inventor 毛兰群苏磊林雨青
Owner INST OF CHEM CHINESE ACAD OF SCI
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