Conjugated bilirubin determination kit

A combination of bilirubin and kit technology, applied in the field of detection of combined bilirubin in serum, to achieve good stability, eliminate interference, and high accuracy

Active Publication Date: 2021-11-09
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Recently, bilirubin oxidase assay kits have gradually entered into clinical practice. However, there are few studies on the determination of conjugated bilirubin at present. Conjugated bilirubin assay kits for clinical needs are very necessary

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0053] Embodiment 1-5 A kind of conjugated bilirubin assay kit

[0054] The components and dosage of the conjugated bilirubin assay kit are shown in Table 1.

[0055] Table 1

[0056]

Embodiment 6

[0065] The detection of embodiment 6 combined bilirubin assay kit

[0066] Using the Hitachi 7080 automatic biochemical analyzer, follow the steps below for detection:

[0067] (1) Basic parameters:

[0068] 1) Reaction method: two-point endpoint method; 2) Reaction temperature: 37°C; 3) Main wavelength: 450nm; 4) Secondary wavelength: 540nm; 5) Reaction time: 10min; 6) Sample / reagent: 1 / 25;

[0069] (2) Determination:

[0070] 1) Reagent preparation: mix reagent 1a and reagent 1b evenly, which is reagent 1; reagent 2 is used directly;

[0071] 2) Operation steps: as shown in Table 2;

[0072] Table 2

[0073]

[0074]

[0075] 3) Result calculation:

[0076] Bc (μmol / L) = ΔA determination × calibrator concentration / ΔA calibration.

[0077] According to the detection method of Example 6, the conjugated bilirubin test kit prepared in Examples 1-5 and Comparative Examples 1-4 is detected, and the linear range, accuracy, precision, stability and anti-interference etc....

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Abstract

The invention discloses a conjugated bilirubin determination kit. The conjugated bilirubin determination kit comprises a reagent 1 and a reagent 2 which are independently packaged, wherein the reagent 1 is composed of a reagent 1a and a reagent 1b which are independently packaged; the reagent 1a is prepared from potassium hydrogen phthalate, sodium fluoride, disodium ethylene diamine tetraacetate, p-toluene sulfonic acid, N-acetylcysteine, laminarin, raffinose and a surfactant; the reagent 1b comprises potassium hydrogen phthalate, ascorbic acid oxidase, laminarin, raffinose, a surfactant and a liquid biological preservative; and the reagent 2 comprises potassium hydrogen phthalate, bilirubin oxidase, laminarin, raffinose, a surfactant and a liquid biological preservative. Compared with the prior art, the conjugated bilirubin determination kit provided by the invention has the advantages of high detection result accuracy, high precision, good stability, strong anti-interference capability and good linear relationship, and meets clinical requirements.

Description

technical field [0001] The invention belongs to the technical field of detection of conjugated bilirubin in serum, in particular to a detection kit for conjugated bilirubin. Background technique [0002] Bilirubin in human serum (serum) includes free bilirubin (UB), conjugated bilirubin (CB) and delta-bilirubin (deltaB), collectively referred to as total bilirubin. During the occurrence, development and recovery of hepatobiliary secretion disorders, the serum CB concentration rises and falls rapidly with the progress of the disease, so the measurement of serum CB becomes an important indicator for monitoring the hepatobiliary secretion function. [0003] At present, the detection methods of bilirubin include diazo reagent method, high performance liquid chromatography, vanadate oxidation method and bilirubin oxidase method, etc., among them, the diazo reagent method is easy to operate, but the method sensitivity and anti-hemolysis The ability is poor; while high performance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 王志商忆文
Owner 浙江夸克生物科技有限公司
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