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Heavy metal colorimetric detection method capable of adjusting catalytic activity of manganous-manganic oxide based on oligonucleotide

A technology of trimanganese tetroxide catalysis and oligonucleotides, which is applied in the measurement of color/spectral characteristics, material analysis by observing the influence on chemical indicators, water testing, etc., and can solve problems such as long analysis time

Active Publication Date: 2019-10-22
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods are sensitive and accurate, they usually require long analysis times, specialized operators and sophisticated instruments

Method used

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  • Heavy metal colorimetric detection method capable of adjusting catalytic activity of manganous-manganic oxide based on oligonucleotide
  • Heavy metal colorimetric detection method capable of adjusting catalytic activity of manganous-manganic oxide based on oligonucleotide
  • Heavy metal colorimetric detection method capable of adjusting catalytic activity of manganous-manganic oxide based on oligonucleotide

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Embodiment 1

[0049] Embodiment 1 of the present invention: a heavy metal colorimetric detection method based on oligonucleotide regulation of manganese tetraoxide catalytic activity, comprising the following steps:

[0050] (1) draw the target heavy metal concentration and absorbance change standard curve, the standard curve is as attached Figure 5 , 6 shown, which includes the following steps:

[0051] A1: Prepare a test sample solution with known target heavy metal (mercury (II) or cadmium (II)) concentration, and obtain A1 product. The specific method is: take about 18 graduated centrifuge tubes of 1.5 mL, and add 10.0 μL of octahedral trimanganese tetraoxide (Mn 3 o 4 NPs) and 10.0 μL of ssDNA (Hg) with a concentration of 20 nM, mixed well and incubated at 30°C for 20 min, then added 10.0 μL of a known concentration of target heavy metal, and then added 10.0 μL of a concentration of 5 mM NaCI salt ion solution , the solution was thoroughly mixed and incubated at 30°C for another 3...

Embodiment 2

[0058] Embodiment 2 of the present invention: a heavy metal colorimetric detection method based on oligonucleotide regulation of manganese tetraoxide catalytic activity, comprising the following steps:

[0059] (1) draw the target heavy metal concentration and absorbance change standard curve, the standard curve is as attached Figure 5 , 6 shown, which includes the following steps:

[0060] A1: Prepare a test sample solution with known target heavy metal (mercury (II) or cadmium (II)) concentration, and obtain A1 product. The specific method is: take about 18 graduated centrifuge tubes of 1.5 mL, and add 10.0 μL of octahedral trimanganese tetraoxide (Mn 3 o 4 NPs) and 10.0 μL of ssDNA (Hg) with a concentration of 20 nM, mixed well and incubated at 30°C for 20 min, then added 10.0 μL of a known concentration of target heavy metal, and then added 10.0 μL of a concentration of 5 mM NaCI salt ion solution , the solution was thoroughly mixed and incubated at 30°C for another 3...

Embodiment 3

[0067] Embodiment 3 of the present invention: a heavy metal colorimetric detection method based on oligonucleotide regulation of manganese tetraoxide catalytic activity, comprising the following steps:

[0068] (1) draw the target heavy metal concentration and absorbance change standard curve, the standard curve is as attached Figure 5 , 6 shown, which includes the following steps:

[0069] A1: Prepare a test sample solution with known target heavy metal (mercury (II) or cadmium (II)) concentration, and obtain A1 product. The specific method is: take about 18 graduated centrifuge tubes of 1.5 mL, and add 10.0 μL of octahedral trimanganese tetraoxide (Mn 3 o 4 NPs) and 10.0 μL of ssDNA (Hg) with a concentration of 20 nM, mixed well and incubated at 30°C for 20 min, then added 10.0 μL of a known concentration of target heavy metal, and then added 10.0 μL of a concentration of 5 mM NaCI salt ion solution , the solution was thoroughly mixed and incubated at 30°C for another 3...

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Abstract

The present invention discloses a heavy metal colorimetric detection method capable of adjusting the catalytic activity of manganous-manganic oxide based on oligonucleotide. According to the method, ato-be-detected sample liquid sample A1 of which the target heavy metal (mercury (II) or cadmium (II)) concentration is known is prepared; a blank reference solution sample A2 is prepared; the absorbance values of the sample A1 and the sample A2 at 450 nm are measured through a microplate spectrophotometer, and an absorbance difference value (deltaA ) is obtained; a target heavy metal concentration and absorbance variation quantity standard curve is drawn according to the absorbance difference value; the regression equation of a relation between target heavy metal concentration and absorbanceis established according to the standard curve; a to-be-detected solution (river water, lake water and tap water) sample C of which the target heavy metal concentration is unknown is prepared; and theabsorbance value of the sample C at 450 nm is measured through the microplate spectrophotometer, and the absorbance difference value of the sample C and the sample A2 is calculated, and the difference value is substituted into the linear regression equation, so that the concentration of a target heavy metal in the sample C is obtained. The method has the advantages of convenience in construction,high sensitivity, high specificity and the like.

Description

technical field [0001] The invention relates to a mercury (II) or cadmium (II) heavy metal detection method, in particular to a mercury (II) or cadmium (II) heavy metal detection method based on an oligonucleotide regulating the catalytic activity of trimanganese tetraoxide. Background technique [0002] Heavy metals are bioaccumulative in the food chain and pose serious threats to human health and the natural environment even at very low concentrations. In order to avoid potential health risks, the US Environmental Protection Agency (EPA) has set the allowable concentration of heavy metals in drinking water as 2-15 μg / L. Therefore, the detection of trace heavy metals is of great significance for environmental monitoring, food safety, medical treatment and clinical testing. Traditional analytical methods such as atomic absorption spectroscopy (AAS), atomic fluorescence spectroscopy (AFS), and inductively coupled plasma mass spectrometry (ICP-MS) have been widely used for ul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78G01N33/18
CPCG01N21/31G01N21/78G01N33/1813
Inventor 吴远根王佳佳陶菡王雪郦
Owner GUIZHOU UNIV
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