High-stability single-reagent liquid-state blood ammonia (AMM) detection reagent

Abstract

The invention relates to a blood ammonia (AMM) detection reagent and in particular relates to a high-stability single-reagent liquid-state blood ammonia (AMM) detection reagent. The reagent is mainly prepared from main substances including a buffering solution, LDH (Lactate Dehydrogenase), a heavy metal ion chelating agent, an ionic balance agent, a preservative, alpha-KG, NADH.NA2, a surfactant, a protecting agent and the like. According to the reagent, a single reagent of glutamate dehydrogenase two-point method and kinetic method is established; the utilization operation is effectively simplified and the stability of effective components of a product is guaranteed; the metal chelating agent is properly selected so that the inhibition, caused by the heavy metal ions, on the activity of an enzyme in the reagent is effectively removed; interference on the enzyme is not caused; the protecting agent is properly added so that the stability of the enzyme and the stability of key substrates in the reagent are effectively protected; the substances are added so that the stability of a single-reagent product provided by the invention is effectively guaranteed and the reagent is greatly convenient for utilization and popularization clinically.

Description

technical field [0001] The invention relates to the technical field of blood ammonia (AMM) detection, in particular to a highly stable single-reagent liquid blood ammonia (AMM) detection reagent. Background technique [0002] The ammonia produced by the catabolism of various amino acids in various tissues in the body and the ammonia absorbed from the intestine enters the blood to form blood ammonia. The normal value of blood ammonia is 18-72μmol / L. The production of blood ammonia is divided into endogenous production and exogenous acquisition. The endogenous production is mainly the ammonia produced by the metabolism in the body, which is called endogenous ammonia, mainly from the deamination of amino acids, and partly from glutamine in renal tubular epithelial cells Ammonia from amidolysis. The decomposition of amines can also produce ammonia. The exogenous acquisition is called exogenous ammonia, which is absorbed by the digestive tract in the human body. It includes u...

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Problems solved by technology

Among them, the sensitivity of the diffusion method and Bo's colorimetric method is low, and the accuracy and precision cannot meet the clinical requirements. The ion exchange chromatography method requires a very long time to process. The stability of the results is affected by many factors, and the electrodes need frequent maintenance and replacement. The detection cost and operation requirements are high, and it cannot be popularized in ordinary hospitals.

At present, dry chemical method and enzymatic method are mostly used. These two methods can be directly applied to semi-automatic and fully automatic biochemical analysis instruments. The cost is low and the operation is relatively low. However, dry chemical method requires dry powder Manual processing will undoubtedly increase the complexity of the operation and increase the error of the operation, and once the dry powder is dissolved, the storage time is short, the key components in the reagent are easy to decay, and the stability and accuracy of the test results cannot be guaranteed. The liquid enzyme two-point kinetics method does not need to do preliminary work, and can be used directly with the instrument. However, in order to ensure the stability of the reagents, most of the enzyme two-point method reagents appearing in the market are four reagents or three reagents. Therefore, customers When using, the reagent must be mixed according to the proportion. Once the reagent is unstable after mixing, it must be used as soon as possible. Otherwise, the reagent will attenuate and affect the accuracy of the result. Therefore, an accurate and stable Enzyme two-point method single reagent can greatly simplify the operation process, facilitate clinical application, and has great application prospects

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