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Alpha-hydroxybutyrate dehydrogenase detection reagent with good stability and high accuracy

A technology of hydroxybutyrate dehydrogenase and detection reagents, which is applied in the field of α-hydroxybutyrate dehydrogenase detection, can solve the problems of low accuracy and poor stability, and achieve improved accuracy, improved accuracy and Specificity, effect of improving assay performance

Inactive Publication Date: 2016-06-08
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reagents on the market generally h

Method used

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  • Alpha-hydroxybutyrate dehydrogenase detection reagent with good stability and high accuracy
  • Alpha-hydroxybutyrate dehydrogenase detection reagent with good stability and high accuracy
  • Alpha-hydroxybutyrate dehydrogenase detection reagent with good stability and high accuracy

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Embodiment Construction

[0046] The present invention will be further described below in conjunction with specific embodiment:

[0047] 1) A high-accuracy α-hydroxybutyrate dehydrogenase detection reagent, including reagent R1 and reagent R2, the composition of the reagent R1 and reagent R2 is as follows:

[0048] Reagent R1 contains

[0049] buffer ··············································· 100mmol / L,

[0050] BSA ·················································· 1g / L,

[0051] 2-ketobutyric acid ············································· 4.4mmol / L,

[0052] EMULGEN-707 ·········································· 0.7ml / L,

[0053] hydroxyethylidene diphosphonic acid ······································· 1ml / L-5ml / L,

[0054] Octadecyltrimethylammonium bromide ································· 0.1g / L-1g / L,

[0055] Alkylphenol Ethoxylates (APEO) ······························ 2g / L,

[0056] ascorbate oxidase ······································· 1KU / L-5KU / L,

[0057] Bilirub...

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Abstract

The invention discloses an alpha-hydroxybutyrate dehydrogenase detection reagent with good stability and high accuracy, and relates to the field of alpha-hydroxybutyrate dehydrogenase detection technologies. Components of a reagent R1 of the alpha-hydroxybutyrate dehydrogenase detection reagent comprise buffer solution, BSA (bovine serum albumin), 2-ketobutyric acid, EMULGEN-707, etidronic acid, octadecy trimethyl ammonium bromide, alkylphenol ethoxylates (APEO), ascorbic acid oxidase, bilirubin oxidase, sucrose, trehalose, xanthan gum and preservatives; components of a reagent R2 of the alpha-hydroxybutyrate dehydrogenase detection reagent comprise buffer solution, NADH xanthan gum and preservatives. The alpha-hydroxybutyrate dehydrogenase detection reagent has the advantages that the etidronic acid is added into the reagent R1, so that heavy metal ions can be effectively chelated, and the accuracy of the alpha-hydroxybutyrate dehydrogenase detection reagent can be effectively improved; chemical acid resistant oxidase and the bilirubin oxidase are added into the alpha-hydroxybutyrate dehydrogenase detection reagent, so that interference of ascorbic acid and bilirubin can be removed; the stabilizer BSA, the sucrose, the trehalose and the xanthan gum are added into the alpha-hydroxybutyrate dehydrogenase detection reagent, so that the stability of the alpha-hydroxybutyrate dehydrogenase detection reagent can be improved; the measurement performance of the alpha-hydroxybutyrate dehydrogenase detection reagent can be obviously improved by the aid of the alkylphenol ethoxylates (APEO) which is a novel surfactant, and the stability of the alpha-hydroxybutyrate dehydrogenase detection reagent can be enhanced.

Description

technical field [0001] The invention relates to the technical field of detection of alpha-hydroxybutyrate dehydrogenase, in particular to a reagent for detection of alpha-hydroxybutyrate dehydrogenase with good stability and high accuracy. Background technique [0002] α-Hydroxybutyrate dehydrogenase (α-HBDH) is an enzyme in the myocardial enzyme spectrum, which is ubiquitous in mammals and mainly distributed in tissues or organs such as myocardial red blood cells, white blood cells and kidneys. α-Hydroxybutyrate dehydrogenase is not an independent specific enzyme, but a general term for LD-1 and LD-2 containing H subunits. α-hydroxybutyrate dehydrogenase ( α-HBDH ) is very closely related to LDH, in fact it is LDH isozyme Type I, because of its α-ketone butyric acid The affinity is high, so when α-ketobutyric acid is used as the substrate, the activity of LDH measured is called α-hydroxybutyrate dehydrogenase activity. Increased α-HBDH activity is common in acute myoc...

Claims

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Application Information

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IPC IPC(8): G01N21/77
CPCG01N21/77G01N2021/775
Inventor 甘宜梧赵新王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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