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Construction method and application of surface protein-embossed self-energized biological fuel cell sensor

A biofuel cell and surface protein technology, applied in the field of biosensors, can solve problems such as poor selectivity, complicated operation, and low sensitivity, and achieve the effects of large construction probability, simple operation, and low cost

Inactive Publication Date: 2017-03-22
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of low sensitivity, poor selectivity, high cost, complicated operation and poor stability in the existing analytical methods for detecting glycoproteins, the purpose of the present invention is to provide a method for constructing a surface protein imprinted self-powered biofuel cell sensor , the method is simple and low cost, and the constructed biofuel cell sensor has high selectivity for glycoprotein recognition, and no external energy supply is required for molecular recognition

Method used

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  • Construction method and application of surface protein-embossed self-energized biological fuel cell sensor
  • Construction method and application of surface protein-embossed self-energized biological fuel cell sensor
  • Construction method and application of surface protein-embossed self-energized biological fuel cell sensor

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Experimental program
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Embodiment 1

[0058] Step (1) Preparation of protein imprinted macroporous monolayer molecularly imprinted polymer (MIP) electrode:

[0059] First, the carbon electrode was washed three times with 0.1M NaOH and secondary water, and then the electrode was immersed in a mixed solution of γ-methacryloxypropyltrimethoxysilane (γ-MAPS) and methanol (volume Ratio 1:9) in 8h. Finally, the electrodes were rinsed with methanol and secondary water respectively to remove residual reagents. 1 mg of vinylphenylboronic acid (VPBA) was first dissolved in 100 μL of polyethylene glycol 200 (PEG200) and 94 μL of 0.2M Na at pH=9.3 2 HPO 4Mix the solution and sonicate for 5min. Then, 6 μL of 10 mg / mL horseradish peroxidase (HRP) aqueous solution, 1 mg of benzoin dimethyl ether, 300 μL of PEG200 and 100 μL of polyethylene glycol diacrylate (PEGDA) were sequentially added to the above mixed solution and stirred at room temperature for 10 min to obtain prepolymer solution. The resulting prepolymer solution (...

Embodiment 2

[0073] Step (1) Preparation of protein imprinted macroporous monolayer molecularly imprinted polymer (MIP) electrode:

[0074] In order to prepare a macroporous single-layer MIP electrode imprinted with alpha-fetoprotein (AFP), 16 μL of AFP solution (1 mg / mL) dissolved in PBS solution at pH = 7.5 was added to the above-mentioned steps instead of HRP when preparing the template solution. In the prepared template solution, the other steps are the same

[0075] Step (2) synthesis of aminophenylboronic acid (APBA) / bilirubin oxidase (BOD) / carbon nanotube (CNT) nanocomposite:

[0076] Same as embodiment 1 step (2).

[0077] Step (3) Preparation of AFP-imprinted MIP biocathode:

[0078] Same as step (2) of Example 1, except that HRP is replaced by AFP of equal concentration.

[0079] Step (4) prepares the preparation of the biological anode of thionine / graphene / glucose dehydrogenase:

[0080] Same as step (4) of embodiment 1.

[0081] Step (5) Preparation of small imprinted carb...

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Abstract

The invention discloses a construction method and application of a surface protein-embossed self-energized biological fuel cell sensor. The method comprises the steps that the surface of a carbon electrode is coated with a molecularly-imprinted polymer, specific protein is labeled with a phenylboronic acid-bilirubin oxidase-carbon nanotube nanocomposite after being adsorbed to the surface of the molecularly-imprinted polymer, and then a surface protein-embossed biological cathode is obtained; the surface of a carbon electrode is modified with a thionine-graphene-glucose dehydrogenase compound, and then a biological anode is obtained; the surface protein-embossed biological cathode, the biological anode and parts including a PMDS electrolytic tank and external resistors are assembled, and then the biological fuel cell sensor is obtained. According to the sensor, the high selectivity and sensitivity to specific glycoprotein are achieved in a compound system where multiple proteins exist to generate interference, external energy supply is not needed during molecular recognition, and large-scale production and application requirements are met.

Description

technical field [0001] The present invention relates to a method for constructing a self-powered biofuel cell sensor, in particular to a method for constructing a surface protein imprinted self-powered biofuel cell sensor, and the surface protein imprinted self-powered biofuel cell sensor is used in specific The invention relates to sex detection and tracking of glycoproteins in complex systems; it belongs to the technical field of biosensors. Background technique [0002] Glycoproteins play important roles in regulating biological processes such as bacterial pathogenic mechanisms, inflammatory responses, and cancer cell metastasis. They are important tumor biomarkers for clinical diagnosis and thus convey information about the physiological state of cells. Currently, various methods including mass spectrometry, immunoassay, electrophoresis, and affinity chromatography are developed to detect and quantify glycoproteins. However, most of the above methods require expensive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403G01N27/327
CPCG01N27/3277G01N27/403
Inventor 邓留欧阳江韩雅静刘又年
Owner CENT SOUTH UNIV
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