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A dual-emission silica fluorescent probe in the detection of cu 2+ application in

A silicon dioxide, fluorescent probe technology, applied in fluorescence/phosphorescence, measurement device, material excitation analysis, etc., can solve the problems of time-consuming, high cost, complexity, etc., and achieve simple and convenient operation, avoid errors, and easy to save. Effect

Inactive Publication Date: 2021-02-05
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are often complex, time-consuming and costly

Method used

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  • A dual-emission silica fluorescent probe in the detection of cu <sup>2+</sup> application in
  • A dual-emission silica fluorescent probe in the detection of cu <sup>2+</sup> application in
  • A dual-emission silica fluorescent probe in the detection of cu <sup>2+</sup> application in

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Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Dual emission silica fluorescent probe (TCPP@SiO 2 / CDs) synthesis

[0031] 1) Synthesis of 5,10,15,20-tetrakis(4-carboxymethyl phenyl)porphyrin (TCPP-OMe): first steam pyrrole by vacuum distillation, add 6.7mL in the constant pressure dropping funnel (0.1mol) newly steamed pyrrole and 30ml propionic acid for use. Add 250 mL of propionic acid and 16.5 g of methyl p-formylbenzoate (0.1 mol) into a 500 mL three-neck round bottom flask, stir rapidly in an oil bath and heat to 140° C. or slightly boil. Then add the mixed solution in the constant-pressure dropping funnel dropwise within 30 minutes, and continue the reflux reaction for 1 hour after the dropwise addition is completed. After cooling to room temperature, put it in a refrigerator at -4°C and let it stand overnight, and filter it with a Buchner funnel to obtain a crude product. The crude product is washed 2-3 times with secondary water and absolute ethanol, respectively. Dry in vacuo to obtain a deep ...

Embodiment 2

[0041] Embodiment 2 fluorescence analysis method detects Cu 2+

[0042] Will TCPP@SiO 2 / CDs were added to the phosphate buffer solution with pH 5 to prepare 50mg / L fluorescent probe solution.

[0043] Sample solution: configure a series of different concentration levels (10 -9 M, 10 -8 M, 10 -7 M, 10 -6 M, 10 -5 M, 10 -4 M, 10 -3 M) Cu 2+ The solution and the concentration are both 10 -3 M's other metal cation solutions, ready to use.

[0044] Take 2mL fluorescent probe solution in a four-sided transparent quartz cuvette, then add the sample solution to detect Cu 2+ Sample solution to TCPP@SiO 2 / CDs probe fluorescence signal. The slit width of the fluorescence spectrometer is set to 10nm, the excitation light wavelength is set to 365nm, and the emission peak fluorescence intensities at wavelengths of 455nm and 660nm are detected. The results show that the addition of Cu 2+ Afterwards, with Cu 2+ With the increase of the concentration, the fluorescence at 455n...

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Abstract

The invention discloses a dual-emission silica fluorescent probe for detecting Cu 2+ In the application, the dual-emission silicon dioxide fluorescent probe includes silicon dioxide-wrapped porphyrin, and carbon quantum dots attached to the outer surface of silicon dioxide. The dual emission silica fluorescent probe for Cu 2+ Has good selectivity. Using Cu 2+ The fluorescence quenching effect on carbon quantum dots is used as the response signal, while the porphyrin is encapsulated by silica and is not affected by Cu 2+ effect, as a stable reference signal, enabling Cu 2+ The fluorescence ratio method detection can effectively avoid the errors caused by the concentration of fluorescent probes and the environment.

Description

technical field [0001] The invention relates to a dual-emission silicon dioxide fluorescent probe in the detection of Cu 2+ in the application. Background technique [0002] Copper is a transition element essential to human health. This element works with certain proteins to produce many enzymes that are essential to life. Copper is also a micronutrient in all known forms of life, where it has functions ranging from bone formation and cellular respiration to connective tissue development. However, if left unregulated, copper can cause disturbances in cellular homeostasis, which can lead to severe neurodegenerative diseases such as Menkes', Wilson's and Alzheimer's diseases. In recent years, copper has also been suspected of causing liver damage in infants. [0003] Due to Cu 2+ Widely used in agriculture and industry, copper contamination and its potential toxic effects on humans continue to be a challenging issue worldwide. Therefore, it is of great significance to de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 卢小泉张彩中牛琦霞李学梅张雪红武亚丽陕多亮
Owner NORTHWEST NORMAL UNIVERSITY
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