Test paper for detecting uric acid content range in urine

A uric acid and test paper technology, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of limited detection sensitivity and accuracy, large blood volume required for measurement, The lower limit is limited and other problems, to achieve the effect of short detection response time, short response time and high accuracy

Inactive Publication Date: 2017-02-15
ZHUHAI HEFAN MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the problems of this method are that special devices are required, the measurement requires a large amount of blood, takes a long time, and is expensive, which is generally only possible in large hospitals.
[0004] Chinese patent application CN200610033783.9 discloses a test paper for detecting the range of uric acid content in urine, but the test paper has been found to have a limited detection limit through experimental research, and the lowest detection limit is only 200 μmol / L. often cannot be detected correctly
Its detection sensitivity and accuracy are limited

Method used

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  • Test paper for detecting uric acid content range in urine
  • Test paper for detecting uric acid content range in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Soak the test paper base strip in a mixed solution containing uricase, horseradish catalase, bilirubin oxidase, bamboo fiber and color developer, wherein the concentration of uricase is 0.4-0.7g / L, spicy The use concentration of root catalase is 0.15g / L, the use concentration of bilirubin oxidase is 0.0.1g / L, the use concentration of bamboo fiber is 0.01g / L, and the color developer is 0.01M tetramethyl benzidine. Shake for 60 minutes, so that all components are adsorbed on the base material together, take out the base material and air dry it at low temperature, cut it into test strips of a certain size, and store them in an airtight and light-proof plastic bag at low temperature. A test strip used to detect the range of uric acid in urine.

Embodiment 2

[0022] Soak the test paper base strip in a mixed solution containing uricase, horseradish catalase, bilirubin oxidase, bamboo fiber and chromogenic agent, wherein the concentration range of uricase is 0.7g / L, horseradish The use concentration range of catalase is 0.2g / L, the use concentration range of bilirubin oxidase is concentration 0.1g / L, the use concentration range of bamboo fiber is 0.05g / L, and the chromogen is 0.1M four Methylbenzidine. Shake for 60-90min, so that all the components are adsorbed on the base material together, take out the base material and air-dry it at low temperature, cut it into test strips of a certain size, and store them in an airtight and light-proof plastic bag at low temperature. Get a test strip that can be used to detect the range of uric acid in urine.

Embodiment 3

[0024] Soak the test paper base strip in the mixed solution containing uricase, horseradish catalase, bilirubin oxidase, bamboo fiber and color developer, wherein the concentration of uricase is 0.4g / L, horseradish catalase The use concentration of catalase is 0.15g / L, the use concentration of bilirubin oxidase is 0.01g / L, the use concentration of bamboo fiber is 0.01g / L, and the chromogen is 0.02 N,N-dimethyl Aniline. Shake for 60 minutes, so that all components are adsorbed on the base material together, take out the base material and air dry it at low temperature, cut it into test strips of a certain size, and store them in an airtight and light-proof plastic bag at low temperature. A test strip used to detect the range of uric acid in urine.

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Abstract

The present invention relates to a test paper strip for detecting the uric acid concentration in urine by using a color reaction. According to the present invention, an enzyme coloring agent, bilirubin oxidase and bamboo fibers are adhered on a test paper substrate, and the main components of the enzyme coloring agent of the test paper strip comprise uricase, horseradish peroxidase, and a coloring agent.

Description

[0001] Technical field [0002] The invention relates to a test paper strip for detecting the concentration of uric acid in urine by using color reaction, which is used for quantitatively and rapidly measuring the level of uric acid in urine. Background technique [0003] Uric acid is the oxidative decomposition product of purine base, which has no physiological function in the human body. The main sources of purine in the human body are diet, tissue decomposition and de novo synthesis. Uric acid is the end product of purine metabolism in humans. Under normal circumstances, it is mainly excreted by the kidneys, accounting for about 2 / 3, and the rest is excreted through the intestines, skin, and hair. Currently, blood uric acid is mainly tested on large blood biochemical analyzers. However, the problems of this method are that special devices are required, the measurement requires a large amount of blood, takes a long time, and is expensive, which is generally only possible ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 和光学樊伟许绍衡贾旭炜刘宇鹏柴琳
Owner ZHUHAI HEFAN MEDICINE
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