42 results about "Myrothecium roridum" patented technology

The invention provides a method for culturing monkey head mushrooms. The method comprises the following steps of 1) preparing production mother strains; 2) performing stock culture; 3) inoculating and germinating fungi; 4) fruiting and harvesting, wherein in the step of preparing production mother strains, citric acid is used for acidizing; in the step of performing stock culture, table vinegar is used for acidizing; in the step of inoculating, stock is broken, and added with nutritious supplementary for mixing and fusion for inoculating and germinating the fungi. The monkey head mushrooms are cultured by utilizing the characteristics of the monkey head mushrooms, and after broken hypha is fused, the regeneration and recombination capacity of the hypha is high; the monkey head mushrooms cultured by the method are large in shape, high in yield, compact in tissues, tough and crispy in mouthfeel, and each monkey head mushroom is about 1 kilogram and is 36 kilograms to the maximum.

The invention belongs to the technical field of microorganisms and specifically relates to a hericium erinaceus HT-3 with a preservation number of CCTCC No: M 2018567. The invention also relates to ascreening method of the hericium erinaceus HT-3. The screening method comprises the following steps: obtaining a purified strain containing a hericium erinaceus tissue block; performing fermental cultivation on the strain; extracting ergothioneine from cells of fermentation liquor mycelium; and determining the content of the ergothioneine in fermentation liquor. The hericium erinaceus strain screened by the invention has a high yield of the ergothioneine, and the adopted screening method is simple in process, convenient in operation and low in production cost.

The invention discloses a submerged fermentation culturing method for a medicinal hericium erinaceus mycelium liquid; compared with a common edible hericium erinaceus, the obtained hericium erinaceus mycelium contains five cancer-fighting substances, seven vitamins, three active cellulases, 18 amino acids, trace elements essential to the human body and on the like except for polysaccharide and polypeptide, wherein contents of cystine, histidine and arginine with larger medicinal values are large; and the 6-7 amino acid identifying points and the protein content (16.65%) of the obtained hericium erinaceus mycelium are respectively 3 times and 1.8 times larger than those of other hericium erinaceus. Infrared spectrum also proves that the hericium erinaceus mycelium also contains a special cancer-fighting organic acid, which is not available in the common hericium erinaceus, except for a hydroxyl ammonia compound. Compared with the traditional manual solid culturing method, the submerged fermentation culturing method disclosed by the invention has the advantages as follows: the breakthrough from manual culture of the hericium erinaceus mycelium to standard and industrial production of the hericium erinaceus mycelium is achieved, the production period is reduced from the 52 days of the traditional culturing method to 14 days, energy is saved and consumption is reduced by 30%, and production cost is reduced by 70%.

The invention discloses a myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 and an application thereof. The nucleotide sequence of the myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 is as shown in SEQ ID NO. 1. According to the invention, a transcriptome sequencing result is used to predict a gene sequence, a cDNA library is used as a template,and amplification is carried out to obtain the myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1. The myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 can efficiently assist saccharomyces cerevisiae in resisting trichothecene toxins, and lay a molecular biological foundation for improving the trichothecene-resistant capacity of saccharomyces cerevisiae in the later period, improving the heterologous expression level of trichothecene toxins and obtaining novel trichothecene toxins.

The invention discloses a bioconversion mycelium medium, bioconversion mycelium and a bioconversion mycelium extract, the bioconversion mycelium medium is produced by adding traditional Chinese medicine earthworm into an agricultural and sideline product culture medium, the bioconversion mycelium is a product obtained by inoculating hericium erinaceus into the bioconversion mycelium medium for mycelium conversion culture, the bioconversion mycelium extract is a product obtained by water extraction treatment, and the bioconversion mycelium extract has anticoagulation effect which is obviously better than the anticoagulation effect of hericium erinaceus mycelium or earthworm, and can be used for the preparation of anticoagulant drugs.

The invention discloses a myrothecium roridum A553 trichothecene synthase gene Tri5 promoter and application thereof. The nucleotide sequence of a promoter is shown in SEQ ID NO.1. According to the promoter, through a chromosome walking technology, the upstream promoter sequence of the Tri5 gene is obtained, the core region of the promoter is predicted, and the myrothecium roridum A553 trichothecene synthase gene Tri5 promoter Tri5P is obtained. By means of the promoter, expression of a hygromycin resistance gene can be efficiently started, the starting efficiency is close to that of a constitutive promoter TEF1, and a molecular biology basis is laid for increasing the yield of trichothecene toxin and obtaining more novel trichothecene toxin with high activities through transcriptional regulation and heterologous expression in the later period.

The invention discloses a culture medium of hericium erinaceus, a bio-converted mycelium, an extract of the bio-converted mycelium, and an application of the extract. The culture medium is prepared by adding a traditional Chinese medicine, sculellaria barbata, to a base culture medium and includes the base culture medium and the sculellaria barbata. 100 parts of the culture medium includes 50-70 parts of the base culture medium and 30-50 parts of the sculellaria barbata, wherein the base culture medium includes, by weight, 350-490 parts of wheat bran, 100-140 parts of corn flour, 45-63 parts of grain bran, 5-7 parts of white sugar and 400-600 parts of water. The bio-converted mycelium is a product that is prepared by inoculating the hericium erinaceus on the culture medium through mycelium conversion. The extract of the bio-converted mycelium is prepared by water-extracting and then pressure-reduced-concentrating the hericium erinaceus mycelium and finally performing vacuum freeze-drying. The extract of the bio-converted mycelium has the effect of resisting gastrointestinal cancer, wherein the effect is significantly better than that of the hericium erinaceus mycelium and the sculellaria barbata independently, so that the extract can be used for preparing an anti-gastrointestinal cancer medicine.

The invention discloses the usage of Myrothecium roridum, and also discloses a compound pesticide of Myrothecium roridum. Myrothecium roridum has strong and quick special pathopoiesis to Eichhornia crassipes but no pathopoiesis to many main crops, and has high safety.

The invention relates to the technical field of biological weeding, particularly to Myrothecium roridum BC-1 with the preservation number of CGMCC No.11393. The Myrothecium roridum BC-1 is separated from blades of naturally diseased Echinochloa crusgalli (L.) Beauv. and has rapid and efficient pathogenicity for the Echinochloa crusgalli (L.) Beauv.; indoor experiments prove that conidia of the strain BC-1 are sprayed onto the blades of the Echinochloa crusgalli (L.) Beauv., scabs can appear after two days, contiguous scabs can appear on the blades of partial Echinochloa crusgalli (L.) Beauv. after seven days, the blades are withered, and the fresh weight reduction of the Echinochloa crusgalli (L.) Beauv. can be up to 93.7% after 21 days. The fungus strain, namely, the Myrothecium roridum BC-1, is safe to crops such as corn, wheat, rice and the like, has the better control effect on the Echinochloa crusgalli (L.) Beauv., has a certain biological control effect on weeds such as common crabgrass, Setaria viridis and the like, has higher potential for development of a commercial bio-herbicide, and has the very significant economic and social benefits.

The invention provides a liquid submerged culture method of Ergosterol Hericium erinaceus. On the basis of preliminary screening, the strains of Hericium erinaceus growing fast on the solid medium are screened; combined with a physical mutagen, ultraviolet mutagenesis is carried out to obtain a A high-yield ergosterol-producing Hericium erinaceus strain suitable for submerged liquid culture. And through the optimization of the culture medium and culture conditions, the liquid submerged culture method of the Hericium erinaceus is determined, which can shorten the period of industrial production of mycelium and increase the biological yield.

The present invention is selenium-rich hedgehog fungus extract preparation for treating colitis, gastritis and stomach cancer. The hedgehog fungus is used as the selenium element carrier, and through deep liquid fermentation and adding Na2SeO3 .5H2O in the amount of 10-50 mg / 100ml into the mycelium and metabolite of hedgehog fungus, hedgehog fungus culturing product of selenium content of 400-700 meg / 100g is obtained. The hedgehog fungus culturing product is added with excipient and prepared into hedgehog fungus extract preparation, which has the functions of protecting gastric mucosa, improving the nutritive state of gastrointestinal tract, regulating body's immunity, inhibiting hyperpiasia, DNA copying and protein synthesis, inhibiting tumor cell growth and treating cancer.

The present invention discloses a hericium erinaceus culture substrate which consists of the following raw materials in parts by weight: 50-56 parts of cotton seed hulls, 30-36 parts of wood chips, 16-22 parts of wheat skin, 2-8 parts of calcium carbonate, 3-9 parts of calcium superphosphate, 2-8 parts of urea, and 15-21 parts of cow dung. By controlling the ratios of the various raw materials, the overall nutritional elements of the entire culture materials are enriched. The added vitamins can meet the needs of the growth of hericium erinaceus to a maximum extent. By culturing the hericium erinaceus using the culture substrate, the production and quality of the hericium erinaceus can be increased, the cost is relatively low, the operation is easy, and the culture substrate is easy for promotion.

The invention relates to a hericium erinaceus cultivating method and belongs to the technical field of microorganism engineering. The hericium erinaceus cultivating method includes that spawn running temperature is kept low at 18-20 DEG C, artificial scattered light is adopted in the period of budding and sporocarp maturity, and the scattered light is permeable light from a door or window which is shaded. By artificially controlling temperature, humidity, light and ventilation, required growth environment of the hericium erinaceus is achieved, and perennial industrialized production can be realized. The hericium erinaceus cultivating method is applicable to the pharmaceutical industry and the healthcare industry.

The invention relates to a fermentation method for producing a fermentation stock solution which contains hericium erinaceus polysaccharides and hericium erinaceus conversion peptide and has the effects of invigorating the stomach and resisting fatigue by means of hericium erinaceus and a preparation method of a beverage. The raw materials comprise soybean meal, rice protein, hericium erinaceus and the like; the fermentation stock solution containing hericium erinaceus conversion peptide and hericium erinaceus polysaccharides is obtained by using protease produced in the growth process of hericium erinaceus for hydrolyzing the soybean meal and the rice protein in the liquid fermentation process, and the fermentation stock solution is prepared into the beverage finally. The method comprises the steps of culture medium preparation, strain activation, fermentation culture, membrane filtration, beverage preparation and the like. The effect is good, the technology is stable, operation is easy, the production cost is low, and industrialization and marketization are easy.

The invention discloses a hericium erinaceus-containing flavoring and a production method thereof. The hericium erinaceus-containing flavoring comprises, by weight, 15 to 25 parts of chickens' extract, 18 to 22 parts of pepper, 15 to 20 parts of Chinese prickly ash, 20 to 30 parts of table salt, 3 to 5 parts of dry ginger, 1 to 2 parts of tsaoko fruit, 7 to 9 parts of tea seed oil, 0.2 to 0.4 parts of pseudo-ginseng, 0.3 to 0.5 parts of dark plum, 1 to 3 parts of hericium erinaceus, 6 to 8 parts of hawk tea, 15 to 20 parts of starch, 1.5 to 2.5 parts of licorice, 0.3 to 0.5 parts of bunge corydalis herb, 3 to 5 parts of mulberry leaf powder, 2 to 4 parts of haw seed powder, 0.5 to 1.0 part of a spice, 0.3 to 0.5 parts of sunflower floral disc and 5 to 8 parts of wolfberry. The hericium erinaceus-containing flavoring has a delicious taste, is prepared by the simple production processes, comprises the natural raw materials, has a strong fragrance, is rich in nutrients, has a good taste and a strong seasoning function, can effectively remove peculiar smells of food materials such as meat, fishes and seafoods, improves a fresh taste of the food materials, and is a healthy, safe and delicious flavoring.

The present invention discloses an artemisia carvifolia biotransformation mycelium and an extract and a use thereof. A preparation method of the artemisia carvifolia biotransformation mycelium is as follows: hericium erinaceus is inoculated into a hericium erinaceus culture medium and cultured at 20-27 DEG C for 30-40 d, and mycelium is taken out and dried to obtain the artemisia carvifolia biotransformation mycelium; every 100 parts of the hericium erinaceus culture medium comprises 50-70 parts of a basal medium and 30-50 parts of artemisia carvifolia; the basal medium comprises the followingcomponents in parts by weight: 350-490 parts of wheat bran, 100-140 parts of corn flour, 50-70 parts of bran coat and 400-600 parts of water; the artemisia carvifolia biotransformation mycelium contains fatty acid substances and ketosteroid substances; and the fatty acid substances comprise palmitic acid, linoleic acid, oleic acid and stearic acid and the ketosteroid substances comprise ergost-7,22-diene-3-one, stigmasta-4-ene-6-alpha-ol-3-one, praeruptorin, scopoletin and byakangelicin. The extract of the artemisia carvifolia biotransformation mycelium has an anti-helicobacter pylori effect,has the effect obviously better than that of hericium erinaceus mycelium or artemisia carvifolia, and can be used for preparing an anti-helicobacter pylori drug.

The invention discloses polysaccharide-production aerial Hericium coralloides ST21-3 and application thereof to improvement on biological immunity activity. The Hericium coralloides Fullarton-H-ST21-3disclosed by the invention is preserved in the China General Microbiological Culture Collection Center and has the preservation number of CGMCC No.15379. The hericium erinaceus is carried by the Tiangong-2 and Shenzhou 11 spacecrafts and is turned back to the ground after aerospace mutation, original hericium erinaceus on the ground is adopted as reference, strains with relatively high activity are bred, and practice bases can be made for application of aerial hericium erinaceus mycelium polysaccharide to improvement on the biological immunity activity. Meanwhile, an aerial microorganism application range is widened to a certain extent, and blanks of research and development of aerial fungus microorganism functions of foods are made up.

The invention discloses a using method of a culture medium for hericium erinaceus. The using method includes the steps: (1) taking appropriate major ingredients and minor ingredients for mixing to obtain a mixture; (2) adding appropriate acetic acid into the major ingredient and major ingredient mixture, regulating the PH value to 5.2, and filling the regulated acid mixture in a plastic bag; (3) sealing the acid mixture at the temperature of 65-75 DEG C for sterilization for 3.2hours, and inoculating hericium erinaceus strains after cooling; (4) cultivating the inoculated hericium erinaceus strains at the temperature of 23-30 DEG C for 25-30 days prior to moving into a fruiting room for moisturizing and fruiting, and harvesting selenium-rich hericium erinaceus after 15-20 days. By the culture medium, the hericium erinaceus grow vigorously and high in nutritive value, iodine content in the hericium erinaceus is high, culture medium utilization efficiency of the strains is high, biotransformation rate is high, cost is saved, and economic benefit of hericium erinaceus cultivation is improved.

The invention discloses a culture medium of hericium and Chinese yam bioconversion mycelium as well as an extract of the Chinese yam bioconversion mycelium and application thereof. The culture mediumof the hericium comprises the following components in parts by weight: 490-350 parts of Chinese yam, 140-100 parts of wheat bran, 70-50 parts of cavings and 400-600 parts of water. The Chinese yam bioconversion mycelium is a product obtained by introducing the hericium into the culture medium and performing mycelial transformation culture. The extract of the Chinese yam bioconversion mycelium is prepared by performing water extraction on hericium mycelium, then concentrating under reduced pressure and finally performing vacuum freeze-drying. The extract of the Chinese yam bioconversion mycelium has a hypoglycemic effect and can be used for preparing hypoglycemic health products or drugs.

The invention discloses a biological bidirectional-transformation mycelium culture medium, a biological bidirectional-transformation mycelium and a biological bidirectional-transformation mycelium extract. The biological bidirectional-transformation mycelium culture medium is prepared by adding traditional Chinese medicine substance sweet wormwood herb to a subsidiary agricultural product culture medium. The biological bidirectional-transformation mycelium is prepared by performing mycelium transformation culture on the culture medium. The biological bidirectional-transformation mycelium extract is obtained by utilizing a water extraction treatment. The biological bidirectional-transformation mycelium extract has an anti-helicobacter pylori function which is remarkably better than that of hericium exinaceus hypha or the sweet wormwood herb, and can be applied to preparation of anti-helicobacter pylori medicines.

The invention discloses a solid fermentation method of bilirubin oxidase, which has the purposes of low cost, simple production process and convenient enzyme extraction. The solid fermentation method comprises the following steps of: (1) separating and screening myrothecium roridum from soil, and preparing a myrothecium roridum spore suspension; and (2) weighing 3-7 parts by weight of bran, placing the bran in a triangular flask, adding 13-17 parts by weight of distilled water, uniformly agitating the bran and the distilled water by using a glass tick, sterilizing under high pressure at 121-130 DEG C for 20-30 min, cooling, inoculating with 1-5 parts by weight of spore suspension prepared in the step (1), cultivating at 22-31 DEG C for 7-11 days, taking out the triangular flask, adding 50-70 parts by weight of distilled water into the triangular flask, leaching for 2-4 h through table concentrator oscillation, and filtering by using qualitative filter paper to obtain the bilirubin oxidase crude liquid. The invention has the advantages of abundant resources and easy acquisition of the raw materials, low production cost, simple, convenient and feasible enzyme extraction process and energy-saving and environmental-friendly production process.

The invention discloses a novel Hericium erinaceus bacterial strain 1 of Hericium erinaceus, which has the advantages that: the biological yield of the novel Hericium erinaceus mycelium in the Hericium erinaceus bacterial strain 1 which is obtained in a culture method disclosed by the invention is high and can reach 12g / L which is twice of the fermentation production capacity in present factories; and the growth speed of the novel Hericium erinaceus bacterial strain 1 is fast and can reach 5.8mm / d, and the yield is high.

The invention discloses an edible fungus feed for ducks and a preparation method of the feed. The edible fungus feed comprises the following raw materials in parts by weight: 50-55 parts of green hay, 23-25 parts of bean meal, 8-9 parts of beer yeast powder, 2-4 parts of corn gluten meal, 4-6 parts of walnut powder, 1-3 parts of lithospermum, 3-5 parts of hericium erinaceus, 5-6 parts of flammulina velutipes, 4-6 parts of camellia mushroom, 2-3 parts of boxthorn leaves, 1-3 parts of cottonrose hibiscus, 2-4 parts of amaranthus paniculatus, 5-7 parts of rape flower, 7-8 parts of lotus leaves, 0.3-0.5 part of ferrous chloride, 0.2-0.4 part of cobalt chloride, 0.02-0.03 part of vitamin B2, 4-6 parts of light calcium carbonate and 2-4 parts of salt. The edible fungus feed is scientific in formula, rich in nutrients and good in palatability, and contains a large quantity of proteins and trace elements and compound vitamins which are necessary for animal growth; multiple fungi are added in the formula, and thus the edible fungus feed has multiple effects of enhancing the immunity, protecting the liver and invigorating the stomach, and has the advantages of invigorating the appetite of the ducks and promoting the growth of the ducks.

The invention discloses a culture medium for hericium erinaceus, biotransformation mycelium, an extract of biotransformation mycelium and an application of the extract. The culture medium for hericiumerinaceus is formed by adding traditional Chinese medicine fructus ligustri lucidi to basal culture media and comprises the basal culture media and fructus ligustri lucidi, wherein every 100 parts ofthe culture media comprises 50-70 parts of basal culture media and 30-50 parts of fructus ligustri lucidi; each basal culture medium is prepared from components in parts by weight as follows: 350-490parts of wheat bran, 100-140 parts of corn flour, 50-70 parts of bran and 400-500 parts of water. The biotransformation mycelium is a product obtained through mycelium transformation culture after the culture medium is inoculated with the hericium erinaceus. The extract of the biotransformation mycelium is prepared after hericium erinaceus mycelium is subjected to water extraction, reduced pressure condensation and final vacuum freeze drying. The extract of the biotransformation mycelium has a blood glucose reducing function which is superior to that of hericium erinaceus mycelium or fructusligustri lucidi and can be used for preparing a drug for reducing blood glucose.

The invention relates to a nutrition medium for cultivation of hericium erinaceus, in particular to a method for cultivating the hericium erinaceus by using gardenia jasminoides waste. 60-80% of gardenia jasminoides residue, 40-50% of rhizoma polygoni cuspidati residue, 10-35% of yellow ginger waste, 0.3-0.6% of sucrose, 0.5-1.5% of urea and 0.5-1.5% of gypsum are sufficiently and evenly stirred,wherein the water content of a culture medium is controlled within 65%-75%; material stirring and bagging are conducted, 200-1000 grams of the culture medium is put into each bag, and sterilization isconducted immediately; high-pressure steam of 121 DEG C is adopted for sterilization for 30-50 minutes, after the bagged culture medium is cooled and inoculated, inoculation of the hericium erinaceusis conducted when the medium is cooled to 25 DEG C, and the hericium erinaceus is cultured and obtained. According to the method, functional components of the gardenia jasminoides and rhizoma polygoni cuspidati are sufficiently used, the method is beneficial for enhancement of oxidation resistance and health care functions of the hericium erinaceus, and large-scale production is facilitated due to less investment and low cost. A large quantity of gardenia yellow substances in the gardenia jasminoides waste are also used, so that the hericium erinaceus is slightly yellow, and various people eat the nutrition medium conveniently.

The invention discloses a process for improving the hygroscopicity of polyester fibers. The process comprises the following steps: 1) preparation of a liquid culture medium; 2) inoculating: inoculating cellulase strain liquid in the liquid culture medium under the condition that the inoculating volume is 6% of the liquid culture medium, placing the liquid culture medium in an environment at the temperature of 36 DEG C, and standing for 24 h to obtain a treatment stock solution; and 3) treatment: preparing the treatment stock solution with the targeted volume according to step 1) and step 2; and placing to-be-modified polyester fibers in modifying equipment, and soaking for 36 h to obtain the modified polyester fibers. By the process, the activity of the cellulase strain liquid is improvedby a myrothecium roridum seed solution at first, the cellulase strain liquid and the myrothecium roridum seed solution supplement each other and act in a combined manner to modify the surfaces of thepolyester fibers, the hydrophilicity of the surfaces of the polyester fibers is improved effectively, and therefore, the moisture absorption performance is improved.

The invention relates to the technical field of biological weeding, particularly to Myrothecium roridum BC-1 with the preservation number of CGMCC No.11393. The Myrothecium roridum BC-1 is separated from blades of naturally diseased Echinochloa crusgalli (L.) Beauv. and has rapid and efficient pathogenicity for the Echinochloa crusgalli (L.) Beauv.; indoor experiments prove that conidia of the strain BC-1 are sprayed onto the blades of the Echinochloa crusgalli (L.) Beauv., scabs can appear after two days, contiguous scabs can appear on the blades of partial Echinochloa crusgalli (L.) Beauv. after seven days, the blades are withered, and the fresh weight reduction of the Echinochloa crusgalli (L.) Beauv. can be up to 93.7% after 21 days. The fungus strain, namely, the Myrothecium roridum BC-1, is safe to crops such as corn, wheat, rice and the like, has the better control effect on the Echinochloa crusgalli (L.) Beauv., has a certain biological control effect on weeds such as common crabgrass, Setaria viridis and the like, has higher potential for development of a commercial bio-herbicide, and has the very significant economic and social benefits.

The invention discloses a biological bidirectional-transformation mycelium culture medium, a biological bidirectional-transformation mycelium and a biological bidirectional-transformation mycelium extract. The biological bidirectional-transformation mycelium culture medium is prepared by adding traditional Chinese medicine substance sweet wormwood herb to a subsidiary agricultural product culture medium. The biological bidirectional-transformation mycelium is prepared by performing mycelium transformation culture on the culture medium. The biological bidirectional-transformation mycelium extract is obtained by utilizing a water extraction treatment. The biological bidirectional-transformation mycelium extract has an anti-helicobacter pylori function which is remarkably better than that of hericium exinaceus hypha or the sweet wormwood herb, and can be applied to preparation of anti-helicobacter pylori medicines.