Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid

A liquid submerged fermentation and Hericium erinaceus mycelium technology, applied in the field of microbial cultivation, can solve the problems of difficult temperature control of heat, difficult monitoring and control of fermentation parameters, and difficulty in stirring, etc., and achieve the effect of reducing production costs

Active Publication Date: 2012-12-26
山西旺龙药业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly three kinds of production approaches of Hericium erinaceus: 1. Artificial cultivation, the advantage is that it is simple and easy to implement, and the cost is low; the disadvantage is that the cultivation period is long, about 3 months; 2, solid fermentation, cultivate Hericium erinaceus bacteria with solid medium Silk, to obtain secondary metabolites, its advantages are: the medium is simple and widely sourced, and the price is cheap; the fermentation process generally does not require strict aseptic operation; the oxygen supply and temperature during the cultivation process can be controlled by direct air forced ventilation; The treatment of fermentation residue is simple, and it can be directly used as feed or fertilizer; the disadvantages are: the water activity of the medium is low, the medium is uneven and difficult to stir, the growth of bacteria, the absorption of nutrients and the secretion of metabol

Method used

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  • Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid
  • Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid
  • Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid

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Embodiment 1

[0040] A liquid submerged fermentation culture method for medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,

[0041] (1).Optimized screening of strains and isolation of fruiting bodies:

[0042] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;

[0043] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol for 2 minutes, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep the room temperature at 20°C, and make the fruiting body grow slowly. After 2 days, the seeds will scatter and fall into the petri dish, then use tweezers to pick up the fal...

Embodiment 2

[0071] A liquid submerged fermentation culture method for medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,

[0072] (1).Optimized screening of strains and isolation of fruiting bodies:

[0073] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;

[0074] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol to disinfect for 1 minute, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep the room temperature at 20°C, and make the fruiting bodies grow slowly. After 3 days, the seeds will scatter and fall into the petri dish, and then use tweezers ...

Embodiment 3

[0101] A liquid submerged fermentation culture method for medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,

[0102] (1).Optimized screening of strains and isolation of fruiting bodies:

[0103] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;

[0104] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol for 2 minutes, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep the room temperature at 20°C, and make the fruiting body grow slowly. After 2 days, the seeds will scatter and fall into the petri dish, then use tweezers to pick up the fal...

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Abstract

The invention discloses a submerged fermentation culturing method for a medicinal hericium erinaceus mycelium liquid; compared with a common edible hericium erinaceus, the obtained hericium erinaceus mycelium contains five cancer-fighting substances, seven vitamins, three active cellulases, 18 amino acids, trace elements essential to the human body and on the like except for polysaccharide and polypeptide, wherein contents of cystine, histidine and arginine with larger medicinal values are large; and the 6-7 amino acid identifying points and the protein content (16.65%) of the obtained hericium erinaceus mycelium are respectively 3 times and 1.8 times larger than those of other hericium erinaceus. Infrared spectrum also proves that the hericium erinaceus mycelium also contains a special cancer-fighting organic acid, which is not available in the common hericium erinaceus, except for a hydroxyl ammonia compound. Compared with the traditional manual solid culturing method, the submerged fermentation culturing method disclosed by the invention has the advantages as follows: the breakthrough from manual culture of the hericium erinaceus mycelium to standard and industrial production of the hericium erinaceus mycelium is achieved, the production period is reduced from the 52 days of the traditional culturing method to 14 days, energy is saved and consumption is reduced by 30%, and production cost is reduced by 70%.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a method for cultivating medicinal Hericium erinaceus mycelium. Background technique [0002] Hericium erinaceus is named because its fruiting body resembles the head of a little monkey. It is a rare edible fungus with both edible and medicinal value. It is flat in nature and sweet in taste. Cancer, treatment of neurasthenia, for the treatment of digestive system diseases and tumors such as indigestion, gastric ulcer, esophageal cancer, gastric cancer, duodenal cancer, and spout cancer. At present, Hericium erinaceus has been successfully used in existing products, such as the marketed Hericium Jianweiling Capsules, in which Hericium erinaceus is the main ingredient of the product. [0003] At present, there are mainly three kinds of production approaches of Hericium erinaceus: 1. Artificial cultivation, the advantage is that it is simple and easy to implement,...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 史长山姜存敏潘志明王俊俊
Owner 山西旺龙药业集团有限公司
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