41 results about "Microbial respiration" patented technology

The invention discloses a freeze-dried fruit yoghourt block and a preparation process thereof. The freeze-dried fruit yoghourt block comprises the following raw materials in parts by weight: 5-10 parts of yoghourt, 1-2 parts of saccharide, 1-2 parts of milk powder, 1-2 parts of whey powder, 1-2 parts of citric acid, 0.1-0.5 part of starch and 0.1-0.2 part of pectin. According to the invention, fruit grains are mixed with the yoghourt, and processing procedures of quick freezing and freeze drying are carried out, so that the water content in the freeze-dried fruit yoghourt block is reduced, therespiration rate of microorganisms in raw materials is reduced, and the shelf life of the raw materials is prolonged; the quick drying procedure enables the water loss rate in the raw materials to reach 80%-90%, so that the loss rate of nutrient components is reduced; the added water is filtered and is subjected to boiling treatment, and the sterilization effect is high; and meanwhile, macromolecular saccharides in fruits are converted into small molecular substances which can be absorbed more easily, so that the absorption rate of the nutrient components of human bodies is increased.

The invention discloses a preparation method of recycled aggregate concrete, and belongs to the technical field of preparation of building materials. According to the method, calcine lime is dissolvedin water during fermentation, heat release provides temperature required for fermentation, produced calcium hydroxide forms an alkaline environment in the water, silicon dioxide, the main component in rice hull ash, is transformed into silicate to be filled into holes of aggregate filter residues, and in addition, microbial respiration produces silicon dioxide during fermentation. The method hasthe advantages that firstly, produced recycled aggregate is low in porosity, and absorption to water can be reduced; secondly, as methoxyl in methoxytrimethyl silane on the surfaces of the aggregate filter residues is easy to hydrolyze and can be in reaction with ammonia gas to produce methyl ammonia alkane substance, the methyl ammonia alkane substance can perform substitution reaction with potassium ion in the rice hull ash, the recycled aggregate concrete has excellent waterproof effect, and the absorption to water by concrete is reduced, so that scaling of the recycled aggregate concrete caused by freeze thawing and weathering is reduced, the service life is prolonged, and the recycled aggregate concrete has a wide application prospect.

Liquid chemical compositions are disclosed for anaerobic biodegradation, detoxification, and transformation of toxic organic and inorganic compounds in a contaminated geologic media under reducing conditions, including, but not limited to, denitrifying, manganese-reducing, iron-reducing and sulfate-reducing conditions. One such liquid chemical composition includes sodium nitrate in the range of one-fifth (0.2) to four (4) pounds per gallon of the chemical composition; sodium hexametaphosphate or other biologically hydrolyzable ring or linear polyphosphate in the range of one twentieth (0.05) to five (5) pounds per gallon of the chemical composition; a surfactant in the range of 0.01% to 10% by volume of the chemical composition; and a diluent in the form of water.A method is disclosed for anaerobic biodegradation, detoxification, and transformation of toxic organic and inorganic compounds in a contaminated geologic media comprising the steps of pressurizing one or more storage tanks containing a chemical composition using an inert carrier gas; connecting a plurality of quick disconnect valve couplings to one or more pressurized storage tanks; connecting a well to an automatic ball valve for supplying the chemical composition and the inert carrier gas through the well to the contaminated geologic media; and opening and closing the automatic ball valve to dispense the chemical composition and the inert carrier gas under pressure through the well to the contaminated geologic media. Methods are also disclosed for alternating the cycles of redox potential and the predominant microbial respiration pathway within the contaminated geological media.

A method for the phenotypic identification of microorganisms is provided. The method is based on the evaluation of the effects of various compounds (effectors) on the respiratory cycle activity of microorganisms. Measurements are based upon the ability of the microorganism to transport electrons to an external chemical oxidant (a mediator) that is added to the microorganism sample. The mediator interacts with the terminal components of the respiratory pathway and the extent of its consumption is related to the ability of the microorganism to respire. The consumed mediator is subsequently measured electrochemically. Electrochemical signals which are generated in the presence or absence of an effector can be used to generate a signal pattern that is unique to an organism and can be used for identification.

The invention discloses a soil water-retaining agent and a preparation method thereof, which belong to the field of crops production. The method adopts chitosan as a first layer network structure, sodium ethylene sulfonate, 2-diethylaminoethyl methacrylate and the like are added to a network structure, the moisture can be better locked, the respiratory action of microorganisms in the soil is always present, and can assist the water-retaining agent for long-term moisture preservation; in the first layer network structure, arbuscular mycorrhizal fungi and cyanobacteria are simultaneously added and mixed, a microbial respiratory responsive additive and carbon dioxide are acted, soil activity is activated, the microbe growth is stimulated, and nutrient can be provided; zeolite is modified by cetyl pyridine bromide, the water-absorption activity is increased, and moisture adsorption quantity is increased, at the same time, the mechanical properties of the gel are enhanced and not lost by the impact of external force, and the water retention performance is stably exerted. The soil water-retaining agent solves the problems of insufficient nutrition to farmland and short water-retention time efficiency of the current water-retaining agents.

A method for the phenotypic identification of microorganisms is provided. The method is based on the evaluation of the effects of various compounds (effectors) on the respiratory cycle activity of microorganisms. Measurements are based upon the ability of the microorganism to transport electrons to an external chemical oxidant (a mediator) that is added to the microorganism sample. The mediator interacts with the terminal components of the respiratory pathway and the extent of its consumption is related to the ability of the microorganism to respire. The consumed mediator is subsequently measured electrochemically. Electrochemical signals which are generated in the presence or absence of an effector can be used to generate a signal pattern that is unique to an organism and can be used for identification.

A paper-based microfluidic chip anode current detection method for the biological toxicity of water pollutants. First, a paper-based microfluidic chip is fabricated, and then the paper-based microfluidic chip is used to measure the biological toxicity of water pollutants. The principle of chemical detection, on the paper-based microfluidic chip of the screen-printed PDMS hydrophilic microchannel and carbon slurry three-electrode system, a small piece of cellulose membrane is used to conduct the hydrophobic valve on the hydrophilic microchannel to control the release and flow of the liquid It can realize the separation of culture medium and cells, washing and incubating cells with pollutants, so as to reduce the interference when using the anode current of the current signal detection device to quantitatively determine the toxicity of pollutants. , The required sample volume is small, the analysis speed is fast, and it is suitable for biological toxicity detection in remote areas, field water pollution, soil pollution, food safety and space carrying and other occasions.

The invention provides a device for detecting toxicity of a water body. The device comprises a micro-organism culturing device and an electrochemical detection device communicated with the micro-organism culturing device, wherein the electrochemical detection device comprises an electrochemical detection tank, a working electrode, a reference electrode, a counter electrode and a constant-temperature device; the working electrode, the reference electrode and the counter electrode are arranged in the electrochemical detection tank; and the constant-temperature device is used for keeping the electrochemical detection tank at constant temperature. The invention further provides a method for detecting the toxicity of the water body. The detection device provided by the invention is used for detecting inhibition of toxic substances in water on micro-organism respiration through the electrochemical detection device, so that the toxicity of the water body is detected; the detection device is simple in structure; furthermore, concentration correction is not required; a detection result cannot be influenced; simultaneously, the detection device provided by the invention is free from influence of turbidity and colour of the water body, high in detection sensitivity and good in repeatability; and a water sample to be detected is detected by taking a micro-array electrode as a working electrode, so that detection current can be increased, thus, the detection sensitivity is increased.

The invention relates to the technical field of food microorganism detection and discloses a device and method for detecting the total number of colonies in foods. The device comprises a reaction chamber, an electric signal amplifier, an electric signal data acquisition card and an electric signal processor. A proton exchange membrane is arranged in the reaction chamber. The reaction chamber is divided into an anode reaction chamber and a cathode reaction chamber through the proton exchange membrane. A reaction system and electrodes are arranged in each one of the anode reaction chamber and the cathode reaction chamber. The electric signal amplifier is respectively connected to the electrodes in the anode reaction chamber and the cathode reaction chamber. The electric signal data acquisition card is respectively connected to the electric signal amplifier and the electric signal processor. The device and method utilize the principle of electrochemistry and utilizes electrons produced bymicrobial respiration as detection objects. Through the correlation curve of voltage values and the total number of colonies, which is drawn according to the known concentration of microbial broth, the total number of colonies in the sample are obtained in 30min and accuracy is 95% or more.

The invention discloses a microbial fertilizer for improving activity of functional microbes and a preparation method of the microbial fertilizer, and belongs to the field of preparation methods of biological fertilizers. The method comprises the following steps: uniformly stacking organic waste, and performing ventilation fermentation to obtain a decomposed organic fertilizer; performing drying naturally, placing the dried material in a drying room, and performing sterilization at a high temperature; adding the functional microbes, a nutrient auxiliary agent and a conditioning agent, performing uniform adsorption, adjusting the humidity, and performing secondary fermentation; and performing granulation on the finished-product microbial fertilizer, performing air drying, and performing packaging. According to the method provided by the invention, after the sterilization operation is completed, the secondary fermentation is performed, inoculation is performed by using the functional microbes, so that a quantity of impurity microbes is reduced, and the dominant position of the functional microbes is improved; the nutrient auxiliary agent is added into the organic fertilizer, so thatthe survival activity of the microorganisms is improved, improvement of activity of the microorganisms is facilitated, respiratory metabolism to release heat of the microorganisms is promoted, and decomposition of the organic fertilizer is slowed down; and the method solves the problem that functional microbes of a current microbial fertilizer have a poorer survival effect and the microbial fertilizer has a shorter shelf life.

The invention discloses an automatic collecting, replacing and sampling device for soil microorganism breathing gas. The device comprises an incubator, a vacuum pump, an air compressor, a negative pressure controller, a three-way balance pressure valve group, a three-way gas collection switching valve group, an automatic controller, at least two two-way balance pressure valve groups and two-way gas collection valve groups, culture bottles are communicated with gas inlets of two-way gas collection valves in the two-way gas collection valve groups in a one-to-one correspondence manner; gas outlets of two-way gas collection valves in the same group are communicated with independent gas collection ports of the same three-way gas production switching valve; a shared exhaust port of the three-way gas production switching valve group is communicated with a gas outlet of a ventilation three-way valve, a shared gas inlet of the three-way gas production switching valve group is communicated witha sample injection gas pipe, the sample injection gas pipe is communicated with a gas inlet of a sample injection three-way valve, and a gas outlet of the sample injection three-way valve is used forbeing communicated with a gas analyzer. According to the invention, the automatic collection, replacement and sample introduction of the sample gas can be realized, so that leakage and cross contamination of the sample gas are prevented, and the online continuous monitoring of the sample gas is ensured.

A rapid detection of microbial CO 2 A gel test paper for respiration rate and a using method thereof belong to the field of environmental chemistry and microbiology. The main components of the gel test paper include NaOH base, acid-base indicator, glycerin and agar, etc., with two test ranges of high range and low range, which can be used for CO 2 Respiration rate detection. The results of the gel test paper are highly readable. By comparing with the five color indicator cards of yellow, orange, red, deep red and purple, the respiration of the compost or soil can be quickly identified, and it can also indicate the five levels of microbial activity or maturity stage. The test paper of the present invention has good sensitivity and high accuracy, and the color change range of the high-range test paper matches 1-50 mg of CO 2 Respiration rate, low range test strip color change range to match 0.1‑5mg of CO 2 Respiration rate; the method of use is simple and fast, and the sample test can be completed within 30 minutes without the need for expensive testing instruments.

The invention relates to the field of machinery, and in particular provides a device for automatically continuously testing soil microbial respiration. The device comprises a constant temperature culture system, a CO2 initial concentration control and gas sampling system and a CO2 concentration analyzer. The device can adjust CO2 concentration and gas flow and flow velocity in the device at the beginning of a test according to requirements, and has a function of automatically switching different test specimens. By the device, the indoor soil microbial respiration can be quickly and continuously tested on line successfully, and the device can be widely applied to the related fields of soil carbon turnover, microorganisms and the like.

The invention discloses a device for continuous measurement of breathing delta13C of soil microorganisms. The device comprises a sampling system, an analytic system and a control system, wherein the sampling system is used for extracting a gas in a culture bottle and conveying the gas to the analytic system; the analytic system uses a calibration gas to perform calibration so as to obtain contents of CO2 and delta13C; and the control system is used for receiving data obtained by the analytic system, and controlling the sampling system. According to the device disclosed by the invention, by establishing an in-situ cultivation system with 16 incubators, continuous measurement and system integration can be achieved, functions of automatic sample injection and analysis are formed, and isotope ratios of dalta13C-CO2 and CO2 concentrations in the incubators can be measured continuously (0.3Hz); moreover, the device disclosed by the invention has a programmable temperature control function and a function of meeting simulation of a complex situation, and can use field experience for reference to establish an online calibration system and ensure the test accuracy, so that commercial mass production and full automatic operation can be achieved, the labor intensity can be reduced, and the device is quick and convenient to use.