Polysaccharide-production aerial Hericium coralloides ST21-3 and application thereof to improvement on biological immunity activity

A Hericium erinaceus and coral-like technology is applied to the polysaccharide-producing space coral-like Hericium erinaceus ST21-3 and its application field in improving biological immune activity, which can solve the problems of staying, the development of food space microorganisms is still in its infancy, and the like, To achieve the effect of broadening the scope of application

Active Publication Date: 2018-11-06
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, domestic and foreign scholars' research on Hericium erinaceus mainly focuses on the optimization of the extraction conditions of polysaccharides and the analysis of the structure of the substances contained in them. For space pathogens, saprophytes and pharmaceutical applications, the development of food space microbes is still in its infancy

Method used

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  • Polysaccharide-production aerial Hericium coralloides ST21-3 and application thereof to improvement on biological immunity activity
  • Polysaccharide-production aerial Hericium coralloides ST21-3 and application thereof to improvement on biological immunity activity
  • Polysaccharide-production aerial Hericium coralloides ST21-3 and application thereof to improvement on biological immunity activity

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Experimental program
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Embodiment 1

[0043] Embodiment 1, the screening of Hericium erinaceus in space

[0044] 1. Space mutagenesis of bacterial strains

[0045] The ground Hericium erinaceus GT21 was carried back by Tiangong 2 and Shenzhou 11 spacecraft, and several strains of space mutagenesis were obtained.

[0046] 2. Activation of strains

[0047] Take the space-mutated strains frozen in the ultra-low temperature refrigerator at -80°C and the preserved strains of Hericium erinaceus GT21 glycerol tubes, dip a small amount of bacterial solution with the inoculation loop and inoculate them on the slant of the YEPD medium test tube, and cultivate them at 28°C for 6 days , used for subsequent experiments after continuous activation for 3 generations.

[0048] 3. Expansion of strains

[0049] The space-mutated strain activated for 3 generations in step 2 and Hericium erinaceus GT21 were inoculated on the slant of the Erlenmeyer flask of YEPD medium, and cultured at 28°C for 6 days.

[0050] 4. Isolation and p...

Embodiment 2

[0084] Embodiment 2, Hericium erinaceus ST21-3 mycelia polysaccharide determination

[0085] 1. Preparation of mycelium

[0086] Inoculate Hericium coralliforme SST21-3 into the seed fermentation medium, culture at 28°C, shake at 160r / min for 12 days, press 8 times / sec with a sterile homogenizer, beat for 1min, beat the mycelial balls, press 10 The inoculum of % (volume percentage) is moved into the seed fermentation medium, 28 ℃, 160r / min shaking culture 5 days, get fermentation system, filter with three layers of sterile gauze, collect mycelium, the mycelium that obtains is used without After washing with distilled water for 3-4 times, freeze-dry in vacuum.

[0087] 2. Preparation of mycelium crude polysaccharides

[0088] Grind the freeze-dried mycelia in step 1 into powder, weigh 1 g of the sample, add 18 mL of distilled water, extract in a boiling water bath for 2 hours, filter with three layers of gauze, repeat the extraction once with the filter residue, combine the e...

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Abstract

The invention discloses polysaccharide-production aerial Hericium coralloides ST21-3 and application thereof to improvement on biological immunity activity. The Hericium coralloides Fullarton-H-ST21-3disclosed by the invention is preserved in the China General Microbiological Culture Collection Center and has the preservation number of CGMCC No.15379. The hericium erinaceus is carried by the Tiangong-2 and Shenzhou 11 spacecrafts and is turned back to the ground after aerospace mutation, original hericium erinaceus on the ground is adopted as reference, strains with relatively high activity are bred, and practice bases can be made for application of aerial hericium erinaceus mycelium polysaccharide to improvement on the biological immunity activity. Meanwhile, an aerial microorganism application range is widened to a certain extent, and blanks of research and development of aerial fungus microorganism functions of foods are made up.

Description

technical field [0001] The invention relates to a polysaccharide-producing space coral-shaped Hericium ST21-3 and its application in improving biological immune activity. Background technique [0002] Hericium erinaceus polysaccharides (HEP) are composed of glucose, galactose, and mannose, which are the main chain connected by β-(1,3) glycosidic bonds and the branched chains connected by β-(1,6) glycosidic bonds. The glucan formed is an important functional active substance produced by Hericium erinaceus through fermentation. Hericium erinaceus polysaccharides include polysaccharides in mycelium cells and polysaccharides in fermentation broth. Among them, the polysaccharide of Hericium erinaceus not only has immunomodulatory activity, but also has biological activities such as lowering blood sugar, lowering blood fat, anti-tumor, and anti-oxidation. The immunomodulatory activity of Hericium erinaceus polysaccharides is manifested in: it can increase the activity of natural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04C12N5/0786A23L31/00A23L33/125C12R1/645
CPCA23V2002/00A23L31/00A23L33/125C12N5/0645C12N2500/34C12P19/04C12N1/145C12R2001/645A23V2200/324A23V2250/51
Inventor 郝红炜张红星刘慧谢远红
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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