A biocontrol strains against water hyacinth and its use

A technology for water hyacinth and uses, applied in the field of microbial source biological inhibitors, can solve problems such as water hyacinth, water pollution, and herbicide influence that cannot be completely solved

Inactive Publication Date: 2007-08-01
上海市农业科学院植物保护研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From January to August 2002, there were about 1.84 million tons of water hyacinth in Shanghai. A large amount of manpower, material resources and funds have been invested in water hyacinth management, but the problem of water hyacinth cannot be completely solved.
Water hyacinth can be effectively killed by chemical herbicides, but it should only be used with caution temporarily and during large-scale outbreaks when other methods are ineffective
The first reason is that a large number of chemical herbicides will cause new pollution to the water body, and the second is that the herbicides have a serious impact on other aquatic plants
Manual and mechanical methods not only require a lot of manpower, but also need a lot of money, but they are still the main methods for controlling water hyacinth

Method used

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  • A biocontrol strains against water hyacinth and its use
  • A biocontrol strains against water hyacinth and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Acquisition of Mycobacterium dewwet strain HNSD2003120101

[0067]The present inventor has isolated dozens of microbial strains from water hyacinth in Shanghai and some other areas in China, and determined their pathogenicity to water hyacinth under controlled and natural conditions. After long-term experiments, a strain of Myrothecium roridum (No.: HNSD2003120101) was found, which has a strong and rapid pathogenic effect on water hyacinth. At the same time, the inventors have tested many major crops (including tomato, cucumber, etc.) with the strain, without any pathogenic effect, indicating that the strain has high safety when used in the field.

Embodiment 2

[0068] Example 2 The preparation method of the conidia inoculum of Mycobacterium dewwet varnish HNSD2003120101

[0069] In the present embodiment, two kinds of methods for preparing the conidia inoculum of Myromyces versicolor are provided, specifically as follows:

[0070] method one

[0071] Inoculate the PSA (potato sucrose agar medium) plate center of the Mycobacterium dewwet bacteria block, and culture it under 25°C and dark conditions for 15 days, and many dark green dots, namely conidia, appear on the bacterial colony. The spores were stained with the seed ring, streaked with PSA, inverted, and cultured for 15-20 days at 25°C in the dark, and a large number of conidia could be formed on the plate (Figure 1).

[0072] Method Two

[0073] Put 120 grams of wheat grains or rice into a 500 ml triangular flask, add water according to the ratio of adding 10 ml of water to every 10 grams of wheat or rice grains, sterilize with high-pressure steam at 121°C for 30 minutes, and ...

Embodiment 3

[0074] Example 3 Determination of the 18S rRNA Fragment, ITS1, 5.8S and ITS2 of Myromyces dew varnish HNSD2003120101

[0075] The total DNA of the Mycobacterium dewwet strain HNSD2003120101 of the present invention was extracted by conventional methods, and the ITS of the fungus was amplified with universal primers ITS5 and ITS4 (ITS5: 5'-ggaagtaaaagtcgtaacaagg-3'; ITS4: 5'-tcctccgcttattgatatgc-3') Section, the measured sequence is as follows (SEQ ID NO: 1): AACAAGGTCT CCGTTGGTGA ACCAGCGGAG GGATCATTAC CGAGTTTACA AACTCCCAAA 60

[0076] CCCTTTGTGA ACCTTACCTA TCGTTGCTTC GGCGGGACCG CCCCGGCGCC TTCGGGCAAC 120

[0077] GGAACCAGGC GCCCGCCGGA GAACCCAAAC TCTTATGTCT TTAGTGGTTT TCTCCTCTGA 180

[0078] GTGACACATA AACAAATAAA TAAAAACTTT CAACAACGGA TCTCTTGGTT CTGGCATCGA 240

[0079] TGAAGAACGC AGCGAAATGC GATAAGTAAT GTGAATTGCA GAATTCAGTG AATCATCGAA 300

[0080] TCTTTGAACG CACATTGCGC CCGCCAGTAT TCTGGCGGGC ATGCCTGTTC GAGCGTCATT 360

[0081] TCAACCCTCA GGCCCCCAGT GCCTGGCGTT GGGGATCGGC GTGGGCC...

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Abstract

The invention discloses the usage of Myrothecium roridum, and also discloses a compound pesticide of Myrothecium roridum. Myrothecium roridum has strong and quick special pathopoiesis to Eichhornia crassipes but no pathopoiesis to many main crops, and has high safety.

Description

technical field [0001] The invention relates to a microorganism-derived biological inhibitor, and more specifically, the invention relates to a new bacterial strain, which can be applied to inhibit the growth of water hyacinth, a malignant aquatic weed. Background technique [0002] Water hyacinth is the common name of water hyacinth (Eichhornia crassipes). Water hyacinth is native to Brazil, and it is introduced to all over the world as an ornamental plant because of its beautiful flowers. However, due to its flooding, it has seriously affected the waterway and water quality, and has become one of the top ten harmful weeds in the world. [0003] Water hyacinth was introduced into my country as a flower in 1901. It was popularized and planted as pig feed in the 1950s and 1960s, and then became wild, which eventually led to the flooding of rivers and lakes in the south in recent years. Water hyacinth has now become one of the 16 invasive alien species announced by the State ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00
Inventor 戴富明何祖华储建君陆金萍李群
Owner 上海市农业科学院植物保护研究所
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