Myrothecium roridum and application thereof

A technology of lacquer spot fungus and conidia, which is applied in the field of microbial control, can solve problems such as environmental pollution, low toxicity, and few large-scale industrial applications, and achieve good control effects, rapid pathogenicity, and significant economic and economic benefits. The effect of social benefits

Inactive Publication Date: 2016-03-30
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of weeds in farmland is mainly through the following three methods: first, manual or mechanical control, this method is time-consuming, laborious, costly and unsatisfactory, and the current application range is relatively small; second, chemical control , the method is simple, low cost, quick effect, and is currently the most important method to solve the harm of weeds
However, the long-term use of chemical herbicides not only causes environmental pollution, but also leads to the emergence of highly resistant weeds, which makes the use of chemical agents fall into a vicious

Method used

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  • Myrothecium roridum and application thereof
  • Myrothecium roridum and application thereof
  • Myrothecium roridum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the isolation and identification of dewy paint spot bacteria BC-1

[0030] 1.1 Isolation of strains

[0031] Collect naturally susceptible barnyardgrass leaves in the field, rinse twice with sterile water, cut off the diseased and healthy joints of the diseased leaves, and disinfect the surface with 5% (v / v) sodium hypochlorite solution for 2 minutes, and then use 70% ( v / v) Alcohol surface disinfection for 1 minute, after rinsing with sterile water, transfer these tissues to an empty sterilized petri dish, and dry them in an ultra-clean bench. Spread the dried leaf tissue on the surface of the PDA medium, place it in an incubator at 28°C, and transfer it to a new PDA culture dish after the bacteria grow, produce spores, separate and purify the single spores to obtain pure culture bacteria Strain BC-1. The BC-1 strain was stored in a -20°C refrigerator by the filter paper method.

[0032] 1.2 Identification of strains

[0033] 1.2.1 Morphological ident...

Embodiment 2

[0045] Example 2: Determination of the host range of bacterial strain BC-1

[0046] Prepare the spore suspension with reference to the method in 1.3, and spray it on the corn, wheat and rice plants at the 2-3 leaf stage. After 7 days, observe the safety of the strains on the tested corn, wheat and rice. After 21 days, the aerial parts of the crops were collected, the fresh weight was weighed, and the fresh weight inhibition rate was counted.

[0047] It is clear from the determination of the host range that the strain BC-1 is safe to crops such as corn, wheat and rice; it has a good biocontrol effect on barnyard grass, and the fresh weight inhibition rate after two weeks is about 91%. It has a certain biocontrol effect on weeds such as crabgrass and foxtail.

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Abstract

The invention relates to the technical field of biological weeding, particularly to Myrothecium roridum BC-1 with the preservation number of CGMCC No.11393. The Myrothecium roridum BC-1 is separated from blades of naturally diseased Echinochloa crusgalli (L.) Beauv. and has rapid and efficient pathogenicity for the Echinochloa crusgalli (L.) Beauv.; indoor experiments prove that conidia of the strain BC-1 are sprayed onto the blades of the Echinochloa crusgalli (L.) Beauv., scabs can appear after two days, contiguous scabs can appear on the blades of partial Echinochloa crusgalli (L.) Beauv. after seven days, the blades are withered, and the fresh weight reduction of the Echinochloa crusgalli (L.) Beauv. can be up to 93.7% after 21 days. The fungus strain, namely, the Myrothecium roridum BC-1, is safe to crops such as corn, wheat, rice and the like, has the better control effect on the Echinochloa crusgalli (L.) Beauv., has a certain biological control effect on weeds such as common crabgrass, Setaria viridis and the like, has higher potential for development of a commercial bio-herbicide, and has the very significant economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of microbial control, in particular to a strain of varnish spot fungus, and also relates to the application of the spot varnish fungus. Background technique [0002] Farmland weeds have well-developed root systems and strong ability to absorb water and nutrients. They compete with field crops for conditions such as water, nutrients, light, and space, seriously endangering the yield safety of farmland crops. There are more than 1,400 species of weeds in my country, among which more than 60 species have important impact on the safety of crops such as grain and vegetables in our country. According to statistics, my country's annual grain loss caused by weed damage reaches 200 million tons, accounting for about 11% of my country's total agricultural output. However, on the other hand, with the development and use of high-efficiency chemical herbicides in recent years, although the harm of weeds to agricultural ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P13/02C12R1/645
Inventor 李健李美高兴祥房锋
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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