Liquid fermentation method of hericium erinaceus conversion peptide and hericium erinaceus polysaccharides, beverage prepared from hericium erinaceus conversion peptide and hericium erinaceus polysaccharides and application

A technology of Hericium erinaceus polysaccharide and fermentation method, which is applied in biochemical equipment and methods, microorganism-based methods, food ingredients containing polysaccharide/gum, etc. The effect of accelerating muscle fatigue recovery and process stability

Inactive Publication Date: 2016-08-17
HUBEI NUOKETE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are many patents on the production of soybean peptides and rice peptides, edible fungus products and production processes, there are no reports on the patents of functional products or beverages containing Hericium erinaceus complex polypeptides and Hericium erinaceus polysaccharides at the same time

Method used

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  • Liquid fermentation method of hericium erinaceus conversion peptide and hericium erinaceus polysaccharides, beverage prepared from hericium erinaceus conversion peptide and hericium erinaceus polysaccharides and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Preparation of PDA slant medium and culture of slant bacteria: 200g peeled potatoes, sliced, add 1000mL water, boil for 15-30min, filter to 1000mL, add 20g sucrose, 1g KH2PO4, 1g MgSO4 7H2O, 20g agar; pH 7.0; put into a test tube, 5 mL each, and sterilize at 121°C for 30 minutes to make a slant medium; inoculate Hericium erinaceus strains on the PDA slant medium and cultivate at 25-30°C for 7-15 days to obtain slant bacteria kind;

[0021] 2. Preparation of liquid seed medium and seed cultivation in liquid Erlenmeyer flask: 200g peeled potatoes, sliced, add 1000mL water, boil for 20 minutes, filter to 1000mL, add 10-30g sucrose, 1-3g KH2PO4, 1-3g MgSO4 7H2O1 ;pH 6~8; sterilize at 115~125°C for 15~30min; take 1~3 small pieces of slant strains into the liquid seed medium, and cultivate them at 25~30°C and 150r / min for 7~15 days to obtain seed cultures;

[0022] 3. Fermentation of Hericium erinaceus transformed peptides and Hericium erinaceus polysaccharides: use a 10...

Embodiment 2

[0025] 1. The preparation of PDA slant medium and the cultivation of slant bacteria, and the preparation of liquid seed medium and the cultivation of liquid Erlenmeyer flask seeds are the same as in Example 1;

[0026] 2. Fermentation of Hericium erinaceus transformed peptides and Hericium erinaceus polysaccharides: 100L fermenter with 50% loading factor, 50g of glucose per L medium, 8g of yeast extract, 1:1 mixed nitrogen source of soybean meal and rice protein 20g, sterilized at 121°C for 30min; insert 3.0L of seeds in a liquid triangular flask, temperature 26-28°C, pH 4-5, dissolved oxygen 20%-50%, after 3 days of fermentation, add 1:1 per L of fermentation broth 30g of soybean meal and rice protein mixed nitrogen source (requires water to sterilize), continue to ferment for 3 days, and then add 30g of soybean meal and rice protein mixed nitrogen source at a ratio of 1:1 per L fermentation broth (requires water to sterilize) Sterilization), continue to ferment for 3 days, a...

Embodiment 3

[0029] Preparation of Hericium Hericium Transformed Peptide and Hericium Polysaccharide Beverage: Hericium Hericium Fermented Stock 20L, Purified Water 80L, Sucrose 8kg, Citric Acid 0.4kg, Ascorbic Acid 10g, Filling, Disinfection, Hericium Compound Polypeptide and Hericium Hericium Bacteria polysaccharide beverage products, wherein the sugar content is 8%, the citric acid content is 0.4%, the Hericium erinaceus conversion peptide content is 0.4%, and the Hericium erinaceus polysaccharide content is 0.2%.

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Abstract

The invention relates to a fermentation method for producing a fermentation stock solution which contains hericium erinaceus polysaccharides and hericium erinaceus conversion peptide and has the effects of invigorating the stomach and resisting fatigue by means of hericium erinaceus and a preparation method of a beverage. The raw materials comprise soybean meal, rice protein, hericium erinaceus and the like; the fermentation stock solution containing hericium erinaceus conversion peptide and hericium erinaceus polysaccharides is obtained by using protease produced in the growth process of hericium erinaceus for hydrolyzing the soybean meal and the rice protein in the liquid fermentation process, and the fermentation stock solution is prepared into the beverage finally. The method comprises the steps of culture medium preparation, strain activation, fermentation culture, membrane filtration, beverage preparation and the like. The effect is good, the technology is stable, operation is easy, the production cost is low, and industrialization and marketization are easy.

Description

technical field [0001] The invention relates to a fermentation method containing Hericium erinaceus transformed peptide and Hericium erinaceus polysaccharide stock solution and a preparation method of a beverage with specific effects of anti-fatigue and stomach strengthening, and belongs to the field of microbial engineering and the technical field of health care products. Background technique [0002] Edible fungi refer to a class of fungi that have health effects on the human body, and their medicinal parts include fruiting bodies, mycelium and fermentation liquid. Hericium erinaceus is a fungus used in traditional Chinese medicine for both food and food. It can benefit the five internal organs, promote digestion, nourish, fight cancer, treat neurasthenia, improve human immunity, and resist ulcers. Due to the scarcity of wild Hericium erinaceus at present, artificial cultivation has a long growth period and is greatly affected by external environmental factors, so it is di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12P19/04A23L2/38A23L2/52A23L33/18A23L33/125C12R1/645
CPCA23L2/382A23L2/52C12P19/04C12P21/06A23V2002/00A23V2200/30A23V2200/308A23V2200/302A23V2200/32A23V2200/324A23V2200/328A23V2200/3262A23V2250/55A23V2250/51A23V2250/628A23V2250/032A23V2250/708
Inventor 石振拓刘宇黄小莉
Owner HUBEI NUOKETE PHARMA
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