Preparation method of gossypin enzymatic hydrolysis product coupled protein used for historical relic detection
A technology of cotton cellulose and enzymolysis products, applied in the field of cultural relics detection, can solve problems such as animal immunity, and achieve the effect of speeding up the speed of enzymolysis, shortening the process flow, and improving the degree of enzymolysis
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[0024] Example 1
[0025] 1) Take 10 g of cotton fiber, grind it for 3 minutes and place it in a beaker, add 45 mL of benzene, 20 mL of ethanol, and soak for 6 hours to degreasing the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, defatted cotton fibers are rinsed 5 times with deionized water and dried in an oven at 50 ℃.
[0026] 2) Take 5 g of the dried cotton fiber powder, add 200 mL of 4% acetic acid, heat it in a water bath at 75 ℃ for 12 h, then suction filter, take the precipitate, wash it with deionized water 5 times, and add 200 mL of 7 % Sodium hydroxide, heated in a water bath at 75 ℃ for 12 h, then suction filtered, and the precipitate was washed with deionized water 5 times.
[0027] 3) Take the final precipitate obtained in step 2, add 8% hydrogen peroxide with a material-to-liquid mass ratio of 1:30, heat it in a water bath at 45 ℃ for 5 h, and then suction filter, rinse the precipitate with deionize...
Example Embodiment
[0033] Example 2
[0034] 1) Take 10 g of cotton fiber, grind it for 4 minutes and place it in a beaker, add 50 mL of benzene, 25 mL of ethanol, and soak for 8 hours to degreasing the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, defatted cotton fibers are rinsed 5 times with deionized water and dried in an oven at 50 ℃.
[0035] 2) Take 5 g of the dried cotton fiber powder, add 200 mL of 5% acetic acid, heat it in a 75 ℃ water bath for 12 h, then suction filter, take the precipitate, wash it with deionized water 5 times and add 200 mL of 8 % Sodium hydroxide, heated in a water bath at 75 ℃ for 12 h, then suction filtered, and the precipitate was washed with deionized water 5 times.
[0036] 3) Take the final precipitate obtained in step 2, add 10% hydrogen peroxide with a material-to-liquid mass ratio of 1:30, heat it in a water bath at 45 ℃ for 5 h, and then suction filter, rinse the precipitate with deionized w...
Example Embodiment
[0042] Example 3
[0043] 1) Take 10 g of cotton fiber, grind it for 5 minutes and place it in a beaker, add 55 mL of benzene, 30 mL of ethanol, and soak for 10 hours to degreasing the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, defatted cotton fibers are rinsed 5 times with deionized water and dried in an oven at 50 ℃.
[0044] 2) Take 5 g of the dried cotton fiber powder, add 200 mL of 6% acetic acid, heat it in a water bath at 75 ℃ for 12 h, then suction filter, take the precipitate, wash it with deionized water 5 times, and add 200 mL of 9 % Sodium hydroxide, heated in a water bath at 75 ℃ for 12 h, then suction filtered, and the precipitate was washed with deionized water 5 times.
[0045] 3) Take the final precipitate obtained in step 2, add 12% hydrogen peroxide with a material-to-liquid mass ratio of 1:30, heat it in a water bath at 45 ℃ for 5 hours, and then suction filter, rinse the precipitate with de...
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