A method for preparing cotton cellulose enzymatic hydrolyzate coupled protein for cultural relics detection
A technology of cotton cellulose and enzymatic hydrolysis products, applied in the field of cultural relics detection, can solve problems such as animal immunity, achieve the effects of accelerating the speed of enzymatic hydrolysis, increasing yield, and shortening the experimental process
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Embodiment 1
[0025] 1) Take 10 g of cotton fiber, grind it for 3 minutes, put it in a beaker, add 45 mL of benzene and 20 mL of ethanol, and soak for 6 h to degrease the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, the degreased cotton fibers were rinsed 5 times with deionized water, and dried in an oven at 50 °C.
[0026] 2) Take 5 g of dried cotton fiber powder, add 200 mL of 4% acetic acid, heat in a water bath at 75 °C for 12 h, then filter with suction, take the precipitate, wash it with deionized water for 5 times, and then add 200 mL of 7 % sodium hydroxide, heated in a water bath at 75 °C for 12 h, then suction filtered, and the precipitate was washed 5 times with deionized water.
[0027] 3) Take the final precipitate obtained in step 2, add 8% hydrogen peroxide with a mass ratio of solid to liquid of 1:30, heat it in a water bath at 45 °C for 5 h, then filter with suction, rinse the precipitate with deionized ...
Embodiment 2
[0034] 1) Take 10 g of cotton fiber, grind it for 4 minutes, put it in a beaker, add 50 mL of benzene and 25 mL of ethanol, and soak for 8 hours to degrease the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, the degreased cotton fibers were rinsed 5 times with deionized water, and dried in an oven at 50 °C.
[0035] 2) Take 5 g of dried cotton fiber powder, add 200 mL of 5% acetic acid, heat in a water bath at 75 °C for 12 h, then filter with suction, take the precipitate, wash it with deionized water for 5 times, and then add 200 mL of 8 % sodium hydroxide, heated in a water bath at 75 °C for 12 h, then suction filtered, and the precipitate was washed 5 times with deionized water.
[0036] 3) Take the final precipitate obtained in step 2, add 10% hydrogen peroxide with a mass ratio of solid to liquid of 1:30, heat it in a water bath at 45 °C for 5 h, then filter with suction, rinse the precipitate with deion...
Embodiment 3
[0043] 1) Take 10 g of cotton fiber, grind it for 5 minutes, put it in a beaker, add 55 mL of benzene and 30 mL of ethanol, and soak for 10 h to degrease the fiber. After the treatment, the mixed solution is evaporated under reduced pressure to recover benzene and Ethanol, the degreased cotton fibers were rinsed 5 times with deionized water, and dried in an oven at 50 °C.
[0044] 2) Take 5 g of dried cotton fiber powder, add 200 mL of 6% acetic acid, heat it in a water bath at 75 °C for 12 h, then filter with suction, take the precipitate, wash it with deionized water for 5 times, and then add 200 mL of 9 % sodium hydroxide, heated in a water bath at 75 °C for 12 h, then suction filtered, and the precipitate was washed 5 times with deionized water.
[0045] 3) Take the final precipitate obtained in step 2, add 12% hydrogen peroxide with a mass ratio of solid to liquid of 1:30, heat it in a water bath at 45 °C for 5 h, then filter with suction, rinse the precipitate with deion...
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