Xanthoceras sorbifolia protein peptide and preparation method thereof as well as protein peptide health-care product
A technology for protein peptides and health products, applied in the field of food processing, can solve problems such as waste of resources, and achieve the effects of improving taste, expanding consumer groups, and improving purity and yield.
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Embodiment 1
[0053] Preparation of Carobia Peptides
[0054] Step 1: Grinding the defatted Xanthos sorbifolia cake into granules of ≤80 mesh;
[0055] Step 2: Weigh one kilogram of sorbifolia sorbifolia cake powder, add 8 kilograms of water, adjust the pH value to 8 with sodium hydroxide, and raise the temperature to 35°C;
[0056] Step 3: under stirring at 100 revs / min of rotating speed, add the flavor protease of 0.1%, the trypsin of 0.1%, the neutral protease of 1.5% (the percentages here all refer to taking the weight of Xantho sorbifolia cake as a benchmark), in Enzymolysis for 9 hours under heat preservation and aseptic conditions;
[0057] Step 4: After enzymolysis, heat up to 75°C to inactivate for 20 minutes, adjust the pH value to 7, and after cooling down to room temperature, centrifuge at a speed of 4000 rpm for 10 minutes to collect the filtrate; the filter residue can be used as a food or feed additive;
[0058] Step 5: Make the particle size of the liquid be 60±10 μm throu...
Embodiment 2
[0063] Step 1: Grinding the defatted Xanthos sorbifolium cake to ≤100 mesh particles;
[0064] Step 2: Weigh one kilogram of sorbifolia fruit cake powder, add 15 kilograms of water, adjust the pH value to 7 with potassium hydroxide, and raise the temperature to 60°C;
[0065] Step 3: under stirring at 100 revs / min of rotating speed, add the papain of 0.1%, the bromelain of 0.1%, the alkaline protease of 5% (percentages here all refer to taking Xanthos sorbifolia cake weight as benchmark), in Keep warm and enzymatically hydrolyze for 4 hours under sterile conditions;
[0066] Step 4: After enzymolysis, heat up to 95°C to inactivate for 5 minutes, adjust the pH value to 7, and after cooling down to room temperature, centrifuge at a speed of 2000 rpm for 20 minutes to collect the filtrate; the filter residue can be used as a food or feed additive;
[0067] Step 5: through a colloid mill with a rotating speed of 3000 rpm, the particle size of the liquid is 50±10 μm;
[0068] Ste...
Embodiment 3
[0072] Step 1: Grinding the defatted sorbifolia cake into granules of ≤90 mesh;
[0073] Step 2: Weigh one kilogram of sorbifolia sorbifolia cake powder, add 13 kilograms of water, adjust the pH value to 9 with sodium hydroxide, and raise the temperature to 40°C;
[0074] Step 3: under stirring at 100 revolutions per minute, add 1% trypsin, 1% neutral protease, and 5% alkaline protease (the percentages here all refer to taking the weight of the sorbifolia cake as a benchmark), Enzymolysis for 6 hours under heat preservation and aseptic conditions;
[0075] Step 4: After enzymolysis, heat up to 90°C to inactivate for 10 minutes, adjust the pH value to 7, and pass through a plate and frame filter after cooling down to room temperature to collect the filtrate; the filter residue can be used as a food or feed additive;
[0076] Step 5: Make the particle size of the liquid be 70±10 μm through a colloid mill with a rotating speed of 3000 rpm;
[0077] Step 6: Refining the collecte...
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