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Method for preparing feed nucleotide by waste beer yeast

A technology of beer waste yeast and nucleotides, applied in the direction of microorganism-based methods, biochemical equipment and methods, animal feed, etc.

Inactive Publication Date: 2013-01-02
宜兴市天石饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If the waste yeast is not utilized, it will not only waste resources, but also cause great pollution to the environment and increase the burden on the treatment of discharged wastewater

Method used

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  • Method for preparing feed nucleotide by waste beer yeast
  • Method for preparing feed nucleotide by waste beer yeast
  • Method for preparing feed nucleotide by waste beer yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Yeast wall breaking and RNA enzymatic hydrolysis: Add 8L of beer waste yeast sludge (equivalent to 890g of dry yeast) into a 10L reaction tank, dissolve 90g of solid sodium hydroxide in a small amount of water, and add it to the yeast under stirring Finally, add water to 9L, heat up to 40°C to break the wall of beer waste yeast and extract RNA for 2 hours, then add hydrochloric acid to the extract to make the pH of the extract 6.5, and take samples through the following UV The content of RNA in the extract was determined by spectrophotometry to be 4.14 mg / ml. According to the RNA content obtained by extraction, 2500-5000 activity units of 5'-phosphodiesterase were required per gram of RNA substrate, and determined and added 1460mL of 5'-phosphodiesterase enzyme solution, then adjust the pH value of the enzymolysis solution at 5.6-5.8, control the temperature at 65-70°C, and carry out the enzymolysis reaction for 3 hours; the 5'-nucleus is measured by the following p...

Embodiment 2

[0051] Take the fresh waste yeast sludge from the brewery, and the measured water content is 89%. Weigh out 3636g of yeast sludge (equivalent to 400g of dry yeast), put it in a 10L stainless steel stirring reaction tank, weigh 40g of solid NaOH and dissolve it in water, and add it into the tank while stirring , make the total volume reach 4000ml, heat up to 25°C, continue to stir and react for 3h, then adjust the pH to 6.5 with industrial hydrochloric acid, measure the content of RNA according to the method described in the above example 1, and the RNA substrate needs 5'- Calculation of phosphodiesterase 2500-5000 activity units, add 706ml of 5'-phosphodiesterase solution extracted from malt root, rapidly raise the temperature to 65°C, and enzymatically hydrolyze for 2 hours at the optimum pH 5.6 and optimum temperature 65°C , measure the 5'-nucleotide content according to the phosphorus determination method described in Example 1 above, and finally raise the temperature to 90°...

Embodiment 3

[0056] Take the fresh yeast mud from the brewery, and the measured water content is 89.6%. Measure 800L of fresh yeast mud into the tank through the standard barrel, which is equivalent to 83.2Kg of dry yeast. Add 27.6Kg of 30% NaOH under stirring, and the total volume is 830L. Heat up to 30°C, stir and react for 1.5h, then adjust the pH to 6.0 with 30% industrial hydrochloric acid, measure the content of RNA according to the method described in Example 1 above, and need 2500 g of 5'-phosphodiesterase per gram of RNA substrate Calculated at -5000 activity units, add 130L of 5'-phosphodiesterase solution extracted from malt root, rapidly raise the temperature to 70°C, maintain the optimum pH of 5.6-5.8 and the optimum temperature of 65-68°C for 3 hours, The 5'-nucleotide content was measured according to the phosphorus determination method described in Example 1 above, and finally the temperature was raised to 90° C. and maintained for 10 minutes to stop enzymatic hydrolysis.

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Abstract

The invention discloses a method for preparing feed nucleotide by waste beer yeast. The method includes preparing the waste beer yeast into 5'-nucleotide by wall-breaking, extracting, enzymolysis and the like. The content of RNA (ribonucleic acid) in extract is measured by ultraviolet spectroscopy. According to the extracted RNA content, the amount of 5'-phosphodiesterase to add is determined by calculating 2500-5000 active units of 5'-phosphodiesterase required per g of RNA substrate, and the RNA is enzymatically hydrolyzed into 5'-nucleotide. The method has the advantages that production cycle is short, investment is low, cost is low, product yield is high, the quality is stable, waste is turned into wealth, no pollutant is discharged and the like, and high economic and social values are achieved.

Description

technical field [0001] The invention relates to a method for preparing nucleotides, in particular to a method for preparing feed nucleotides with beer waste yeast. Background technique [0002] There are as many as 50,000 tons of waste yeast eliminated from beer production in my country every year, and the ribonucleic acid (RNA) content in beer yeast cells is as high as 4% to 6% of yeast cells. Ribonucleic acid is composed of four monomeric nucleotides, cytidine (5'-CMP), uridine (5'-UMP), adenylate (5'-AMP) and guanylate (5' -GMP). Nucleotides are connected to the 3' and 5' positions on the five-carbon ribose ring through phosphoric acid, forming long-chain RNA macromolecules consisting of hundreds or even thousands of nucleotides. In the natural environment of cells, RNA Exists with protein in the form of nucleoprotein. [0003] Nucleotides have been used in feed and aquaculture, and nucleotides are still called new feed additives. Many mammals, poultry, fish and shrim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/30A23K1/16C12R1/865
Inventor 薛宏基赵传江邹益东赵亮刁海霞
Owner 宜兴市天石饲料有限公司
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