Sialic acid electrochemical test paper as well as preparation method and detection method thereof

A sialic acid and electrochemical technology, which is applied in the field of sialic acid electrochemical test paper and its preparation and detection, can solve the problems of photochemical colorimetric method, such as large blood collection, high price, and long time required for analysis results

Active Publication Date: 2018-09-18
GUILIN ZHONGHUI TECH DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among these methods, the detection process of high-performance liquid chromatography and chemiluminescence method is cumbersome, and it takes a long time to obtain the analysis results; the photochemical colorimetric method t...

Method used

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  • Sialic acid electrochemical test paper as well as preparation method and detection method thereof
  • Sialic acid electrochemical test paper as well as preparation method and detection method thereof
  • Sialic acid electrochemical test paper as well as preparation method and detection method thereof

Examples

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Embodiment 1

[0090] This embodiment provides a preparation example of a sialic acid test paper to illustrate the sialic acid test paper and preparation method of the present invention.

[0091] Firstly, the working electrode 2-1, the reference electrode 2-2 and the short-circuit electrode 2-3 are made on the 0.25mm thick PET plastic substrate, ie, the insulating substrate 1, using screen printing technology, using conductive carbon ink purchased by Acheson Production, spread the conductive ink on the PET plastic substrate with a thin layer of evenly dispersed ink by screen printing, then put it in an oven at 80°C for 60 minutes;

[0092] Secondly, using screen printing technology to print the insulating varnish on the substrate of the printed electrode system as the electrical insulating isolation layer 3. The insulating varnish was purchased by Acheson, and the insulating varnish was spread on the PET plastic substrate by screen printing with evenly dispersed ink After the thin layer, put it i...

Embodiment 2

[0105] This example illustrates the linear relationship between the concentration of sialic acid and the current, and the method of constructing a calibration curve using the sialic acid test paper prepared in Example 1.

[0106] Collect fresh blood with a packed volume ratio of 45%, prepare blood samples with sialic acid concentrations of 0.5, 1.0, 1.5, 3.0, 5.0, and 7.0 mmol / L, and place the sialic acid test paper prepared in Example 1 on a multi-parameter analyzer Apply 300mV working voltage to both ends of working electrode 2-1 and reference electrode 2-2; take blood with a pipette to contact sampling port 7, blood will automatically draw about 2μL of sample through siphon guide groove 6, and it will be hydrolyzed by pretreatment layer 5. Part of the combined sialic acid reaches the reaction zone and reacts with the reagents in the enzyme reaction membrane 4 to form a current. The current corresponding to the concentration of sialic acid can be quickly detected on the multi-pa...

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Abstract

The invention discloses a sialic acid electrochemical test paper as well as a preparation method and a detection method thereof. The sialic acid electrochemical test paper comprises an insulating substrate, a conductive electrode, an insulated isolation layer, a reaction layer, a pretreatment layer, a siphon sample guide groove and an adhesion spacer; the reaction layer is an enzyme reaction membrane; the enzyme reaction membrane covers the surfaces of a reference electrode and a working electrode of a reaction region and is arranged at the reaction region end of the insulated isolation layer;the pretreatment layer is arranged at the front end of the reaction layer; the reaction layer and the pretreatment layer are arranged in the siphon sample guide groove; the enzyme reaction membrane comprises an enzyme which is reacted with sialic acid and an electron transfer mediator; and slurry of the pretreatment layer contains a sialic acid hydrolytic agent. For the sialic acid electrochemical test paper provided by the invention, by using a method of detecting the sialic acid with a direct electrochemical biosensor principle, by arranging a sample pretreatment structure and combining enzyme in an enzyme layer, the sialic acid in blood is rapidly and thoroughly decomposed into free sialic acid, so that the test sensitivity of the sialic acid is improved, and a method of accurately, rapidly and simply detecting the sialic acid with low cost is realized.

Description

Technical field [0001] The invention relates to the field of electrochemical test paper and electrochemical detection, in particular to a sialic acid electrochemical test paper and a preparation and detection method thereof. Background technique [0002] Sialic acid (SA) is a general term for a class of carboxylated monosaccharide acylated derivatives containing 9 carbon atoms. The main sialic acids in the human body are N-acetylneuraminic acid and N-glycolylneuraminic acid Two are mostly produced by glucose metabolism. Serum sialic acid is derived from the surface of cells and exists in two forms: sugar-bound sialic acid and lipid-bound sialic acid. [0003] The level of sialic acid expression in various tissues during the developmental stage of the body is higher, while it is low in adult tissues. If the level of sialylation in adult tissues increases, it indicates the possibility of pathological changes. In the 1970s, foreign scholars through animal experiments and human tumor...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3272Y02E60/50
Inventor 梁明龙刘勤伍寿胜李中廖姗
Owner GUILIN ZHONGHUI TECH DEV
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