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Green preparation method of yeast glucan

A yeast glucan, green technology, applied in the field of glucan, to achieve the effect of promoting enzymatic hydrolysis efficiency, reducing the residue of impurities, and improving the removal rate of impurities

Active Publication Date: 2019-05-24
CHANGZHOU NO 2 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ultrasonic depolymerization can only reduce the molecular weight of dextran to a limit constant value, while oxidative degradation will introduce impurities such as oxidants into dextran

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A green preparation method of yeast glucan, comprising the steps of:

[0030] Step 1: Add common yeast cell walls into distilled water, stir evenly to form a suspension, heat at a constant temperature for 2 hours, then add tourmaline and hydrogen peroxide, seal and cook for 2 hours, keep warm for 2 hours, take out the tourmaline, and centrifuge to obtain a precipitate;

[0031] Step 2, add the precipitate to distilled water and stir evenly, add a dispersant and heat and stir to form a suspension, then add lye to adjust the pH to 9 to obtain an alkaline suspension;

[0032] Step 3, adding protease and tourmaline to the alkaline suspension, enzymolysis at constant temperature for 4 hours, taking out the tourmaline, centrifuging, and washing with water to obtain insoluble matter;

[0033] In step 4, the insoluble matter was added to acetone for microwave reaction for 2 hours, filtered and freeze-dried to obtain yeast glucan powder.

[0034] The concentration of the yeast ...

Embodiment 2

[0042] A green preparation method of yeast glucan, comprising the steps of:

[0043] Step 1: Add common yeast cell walls into distilled water, stir evenly to form a suspension, heat at a constant temperature for 4 hours, then add tourmaline and hydrogen peroxide, seal and cook for 4 hours, keep warm for 6 hours, take out the tourmaline, and centrifuge to obtain a precipitate;

[0044] Step 2, adding the precipitate to distilled water and stirring evenly, adding a dispersant and heating and stirring to form a suspension, and then adding lye to adjust the pH to 11 to obtain an alkaline suspension;

[0045] Step 3, adding protease and tourmaline to the alkaline suspension, enzymolysis at constant temperature for 6 hours, taking out the tourmaline, centrifuging, and washing with water to obtain insoluble matter;

[0046] In step 4, the insoluble matter was added to acetone for microwave reaction for 5 hours, filtered and freeze-dried to obtain yeast glucan powder.

[0047] The co...

Embodiment 3

[0055]A green preparation method of yeast glucan, comprising the steps of:

[0056] Step 1: Add common yeast cell walls into distilled water, stir evenly to form a suspension, heat at a constant temperature for 3 hours, then add tourmaline and hydrogen peroxide, seal and cook for 3 hours, keep warm for 4 hours, take out the tourmaline, and centrifuge to obtain a precipitate;

[0057] Step 2, adding the precipitate to distilled water and stirring evenly, adding a dispersant and heating and stirring to form a suspension, and then adding lye to adjust the pH to 10 to obtain an alkaline suspension;

[0058] Step 3, adding protease and tourmaline to the alkaline suspension, enzymolysis at constant temperature for 5 hours, taking out the tourmaline, centrifuging, and washing with water to obtain insoluble matter;

[0059] In step 4, the insoluble matter was added to acetone for microwave reaction for 4 hours, filtered and freeze-dried to obtain yeast glucan powder.

[0060] The con...

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Abstract

The invention belongs to the technical field of glucan, and particularly relates to a green preparation method of yeast glucan. The green preparation method includes the steps: 1 adding common yeast cell walls into distilled water, uniformly stirring the common yeast cell walls and the distilled water to form suspension liquid, heating the suspension liquid at constant temperature for 2-4 hours, adding tourmaline and hydrogen peroxide, performing sealed cooking for 2-4 hours, keeping the temperature for 2-6 hours, taking out the tourmaline and performing centrifugation to obtain precipitates;2 adding the precipitates into the distilled water, uniformly stirring the precipitates and the distilled water, adding dispersing agents, heating and stirring the dispersing agents and the distilledwater to form turbid liquid, adding alkaline liquid, and adjusting pH (potential of hydrogen) to be 9-11 to obtain alkaline turbid liquid; 3 adding protease and the tourmaline into the alkaline turbidliquid, performing constant-temperature enzymolysis for 4-6 hours, taking out the tourmaline and then performing centrifugation and washing to obtain insoluble substances; 4 adding the insoluble substances into acetone, performing microwave reaction for 2-5 hours, and filtering and freeze-drying reactants to obtain yeast glucan powder. The yeast cell walls serve as production raw materials, the tourmaline serves as an auxiliary catalyst and an auxiliary oxidizer, and the yield of the yeast glucan is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of dextran, and in particular relates to a green preparation method of yeast glucan. Background technique [0002] At present, yeast β-D-glucan (referred to as yeast β-glucan or yeast glucan) is an immunomodulator (non-specific immune enhancer) with good physiological functions. It has the functions of enhancing immunity, anti-tumor, Antibacterial and anti-inflammatory, anti-oxidation, promotion of wound healing, anti-radiation, lowering blood lipids and other physiological effects, but it is insoluble in water and most other solvents, only slightly soluble in dimethyl sulfoxide, which severely limits the application of yeast dextran . [0003] In order to improve the solubility of yeast glucan, scholars at home and abroad have carried out modification and modification research on it, including chemical and physical methods. The main chemical modification methods are phosphorylation, carboxymethylation, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08C12R1/645
Inventor 戚春建
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL
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