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Process for extracting picroside from rhizoma picrorhizae through fermentation

A technology of berberine and berberine, which is applied in the field of fermentation and extraction of berberine, can solve the problems of unstable process, low repetition rate, and low extraction efficiency of total saponins of berberine, and achieve high content, easy operation, and stable properties. Effect

Inactive Publication Date: 2020-08-14
山东康裕生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many reports in the literature on the method of extracting the total saponins of Rhizoma Rhizoma Pedis, most of which adopt ethanol reflux extraction and then cooperate with macroporous resin as the separation and preparation method of the adsorbent, but the extraction efficiency of total saponins of Rhizoma Rhizome is low, and the process is not good. Stability and low repetition rate technical problems need to be solved urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A process for fermenting and extracting berberine from berberine, comprising the steps of:

[0023] S1. Disperse cellulase, hemicellulase, protease, α-amylase, and pectinase in water to obtain an enzyme preparation; wherein the concentration of cellulase is 12u / g, and the concentration of hemicellulase is 30u / g , the concentration of protease is 1u / g, the concentration of α-amylase is 4u / g, and the concentration of pectinase is 1u / g;

[0024] S2. Add 1 kg of sucrose ester and 2 kg of ammonium oxalate to 150 kg of enzyme preparation and mix evenly, add 20 kg of pulverized Rhizoma Peptides, stir at 35°C for 4 hours at a low speed of 80 r / min, and inactivate at a high temperature to obtain a fermentation product;

[0025] S3. Put 100kg of fermentation product into 150kg of ethanol solution with a mass fraction of 90%, let it stand at room temperature for 20h, ultrasonically treat it for 30min at an ultrasonic frequency of 30kHz, heat and reflux for extraction for 6h, filte...

Embodiment 2

[0029] A process for fermenting and extracting berberine from berberine, comprising the steps of:

[0030] S1. Disperse cellulase, hemicellulase, protease, α-amylase, and pectinase in water to obtain an enzyme preparation; wherein the concentration of cellulase is 18u / g, and the concentration of hemicellulase is 20u / g , the concentration of protease is 3u / g, the concentration of α-amylase is 2u / g, and the concentration of pectinase is 2u / g;

[0031] S2. Add 3 kg of sucrose ester and 1 kg of ammonium oxalate to 100 kg of enzyme preparation, mix evenly, add 40 kg of crushed Rhizoma Peptides, stir at 25°C for 10 hours at a low speed of 60 r / min, and inactivate at a high temperature to obtain a fermentation product;

[0032] S3. Put 100kg of fermentation product into 200kg of ethanol solution with a mass fraction of 80%, let it stand at room temperature for 30h, ultrasonically treat it for 20min at an ultrasonic frequency of 40kHz, heat and reflux for extraction for 4h, filter, an...

Embodiment 3

[0036] A process for fermenting and extracting berberine from berberine, comprising the steps of:

[0037] S1. Disperse cellulase, hemicellulase, protease, α-amylase, and pectinase in water to obtain an enzyme preparation; wherein the concentration of cellulase is 14u / g, and the concentration of hemicellulase is 28u / g , the concentration of protease is 1.5u / g, the concentration of α-amylase is 3.5u / g, and the concentration of pectinase is 1.3u / g;

[0038] S2. Add 1.5 kg of sucrose ester and 1.7 kg of ammonium oxalate to 140 kg of enzyme preparation, mix evenly, add 25 kg of pulverized Rhizoma Peptides, stir at a low speed of 32°C for 6 hours at a stirring speed of 75 r / min, and inactivate at a high temperature to obtain a fermentation product;

[0039] S3. Put 100kg of fermentation product into 160kg of ethanol solution with a mass fraction of 88%, let it stand at room temperature for 23h, ultrasonically treat it for 28min at an ultrasonic frequency of 33kHz, heat and reflux f...

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PUM

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Abstract

The invention discloses a process for extracting picroside from rhizoma picrorhizae through fermentation. The process comprises the following steps: dispersing cellulase, hemicellulase, protease, alpha-amylase and pectinase in water to obtain an enzyme preparation; adding sucrose ester and ammonium oxalate into the enzyme preparation, uniformly mixing, adding crushed rhizoma picrorhizae, stirringat the temperature of 25-35 DEG C for 4-10 hours at a stirring speed of 60-80 r / min, and inactivating at a high temperature to obtain a fermentation product; feeding the fermentation product into an ethanol solution, standing at normal temperature for 20-30 hours, carrying out ultrasonic treatment for 20-30 minutes at the ultrasonic frequency of 30-40 kHz, heating, refluxing and extracting for 4-6hours, filtering, decompressing filtrate, recovering a solvent, concentrating, loading onto a polyamide chromatographic column, washing the polyamide column with water until the polyamide column is colorless, and eluting with a mixed solvent of which the volume is 3 times that of the column bed; and collecting the eluate, concentrating and drying to obtain the picroside.

Description

technical field [0001] The invention relates to the technical field of berberine extracting, in particular to a process for fermenting and extracting berberine from berberine. Background technique [0002] Huhuanglian is a traditional Chinese medicine in my country. Huhuanglian is the dry rhizome of the perennial herb of Scrophulariaceae. It is cold in nature and bitter in taste. Hairy. Stems cylindrical, slightly woody, 15-20cm long. [0003] Huhuanglian is a perennial herb of Scrophulariaceae. It has a similar name to Coptis chinensis. Both of them are bitter cold, clearing away heat and dampness. They are good at removing dampness and heat in the stomach and intestines. At present, it has been found that the chemical components of Coptis chinensis mainly include iridoids, cucurbitacins, phenylethyl alcohol glycosides and phenolic glycosides, and also contain very small amounts of mannitol, berberine alcohol, berberine sterol, vanilla ethyl ketone and Aromatic acid, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08A61K36/80
CPCA61K36/80A61K2236/19A61K2236/333A61K2236/55C07H1/08C07H17/04
Inventor 沈彩芹刘刚刘培冉刘乃兵赵学峰田雪文杨志强张志东
Owner 山东康裕生物科技有限公司
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