55 results about "Cucurbitacins" patented technology

The invention provides a biomarker for diagnosing white matter lesion and application thereof. The biomarker is cholesterol-alpha-D-glucoside. The biomarker cholesterol-alpha-D-glucoside is combined with 6Z,9Z,20-eicosatrienoic acid, ceramide (m18:1(4E) / 24:1(15Z)), ephedrine A, cucurbitacine E, cholesteryl ester 22:6 or ceramide (d18:0 / 24:1(15Z)) to judge whether white matter lesion exists or not. The biomarker for diagnosing the white matter lesion is used for preparing a detection reagent for diagnosing the white matter lesion, and is beneficial for diagnosing whether the white matter lesion tends to exist or not and preventing the white matter lesion in advance.

The invention provides a biomarker for diagnosing white matter lesion and application thereof, and the biomarker is 6Z, 9Z, 20-eicosatrienoic acid. The biomarker 6Z, 9Z, 20-eicosatrienoic acid is combined with ceramide (m18: 1 (4E) / 24: 1 (15Z)), ephedrine A, cucurbitacine E, cholesteryl ester 22: 6 or ceramide (d18: 0 / 24: 1 (15Z)) for judging whether white matter lesion exists or not. The biomarker for diagnosing the white matter lesion is used for preparing a diagnostic reagent for predicting or diagnosing the white matter lesion, and is beneficial for diagnosing whether the white matter lesion tends to exist or not and preventing the white matter lesion in advance.

The invention provides a preparation method of a cucurbitacin type compound. The preparation method comprises the steps of extracting pedicellus melo by using an organic solvent, performing solvent distribution on an extract, and performing silica gel column separation and purification by high performance liquid chromatography on a sample after concentration to obtain a cucurbitacin mixture and a cucurbitaceae type compound. Through an anti-aging yeast model-K6001 yeast cell, an active ingredient, namely the cucurbitacin type compound is proved to be capable of significantly prolonging the replicative life span of yeast, so that the cucurbitacin mixture and the cucurbitaceae type compound can be applied in preparation of medicaments for preventing and treating aging and preventing or / and treating age-related diseases. Simultaneously, the cucurbitacin mixture and the cucurbitaceae type compound can also be applied in preparation of nutritional compositions for delaying aging and preventing age-related diseases. The compound can also be used as a lead compound for optimizing the structure and can be applied in preparation of medicaments for delaying aging and preventing or / and treating age-related diseases. The general formula of the structure of the compound is shown in the specification.

The invention belongs to the technical field of medicine, and particularly relates to an application of tetracyclic-triterpene-like compound-like cucurbitacine B separated and extracted from cucurbitaceous plants as a cancerous inhibitor of protein phosphatase 2A. Cucurbitacine B is taken as a main component in the cancerous inhibitor of protein phosphatase 2A, so that the expression of the cancerous inhibitor of protein phosphatase 2A can be inhibited remarkably. The cucurbitacine B can be applied to independent administration or combined treatment, including combined applications with radiotherapy, chemotherapy, surgical treatment, other growth factor inhibitors and sexual hormone inhibitors, any tumor treatment medicament and method.

The invention relates to a stable liquid curbitacin preparation which contains curbitacin and Brucea javanica oil and can also contain other liquid fatty oil and / or antioxidant. The invention also relates to a medicament taking the stable liquid curbitacin preparation as an active component. The medicament can be the injection, the oral medicament, the dropping pill, the emulsion, the soft capsule, the hard liquid capsule or the suspension. The stable liquid curbitacin preparation has the efficacy of treating the hepatitis, the cirrhosis, the tumour, the cancer and the like; the curbitacin and the Brucea javanica oil can interact synthetically; and the Brucea javanica oil can well solve the curbitacin and prevent the curbitacin from being oxidized.

The invention discloses a prescription for preparing a cucurbitacin oral lipid nano emulsion and a preparation method. The cucurbitacin oral lipid nano emulsion contains cucurbitacin, medium chain triglyceride, soya lecithin, polyoxyethylene 40 hydrogenated castor oil, an antifreezing agent, vitamin E and water. The prepared cucurbitacin oral lipid nano emulsion has good antifreezing capability, Compared with the tablet in the market, the disintegration of the tablet in the gastrointestinal tract is not existed, the dissolution of the medicine is fast, the cucurbitacin oral lipid nano emulsion is widely and uniformly distributed in a easy mode, the acting time is fast, and the bioavailability of the cucurbitacin oral lipid nano emulsion is obviously enhanced. The preparation technology is simple, and can be used for industrial production.

The invention discloses an ethyl acetate layer of methanol extract of Aquilaria hulls for killing cancer cells and treating / preventing cancers and its uses. The extracts of Aquilaria hulls has an significant amount of cucurbitacins and these cucurbitacins are effective in killing cancer cells and treating / preventing cancers.

The invention discloses a hemsleya amabilis triterpene synthetase HcOSC6 gene and engineering bacteria thereof as well as an application to preparation of gourd dienol. The hemsleya amabilis triterpene synthetase HcOSC6 gene has a nucleotide sequence shown in SEQ ID No.1, and the engineering bacteria can express hemsleya amabilis triterpene synthetase HcOSC6. In a culture medium taking D-glucose and D-galactose as carbon sources, the genetically modified engineering bacteria can express hemsleya amabilis triterpene synthetase HcOSC6 in cells, under the catalysis of hemsleya amabilis triterpenesynthetase HcOSC6, an endogenous substrate squalene-2,3-oxide of yeast can be subjected to a cyclization reaction, and gourd dienol is produced and has the yield about 5.5 mg / L. An important way forproducing the gourd dienol is provided, and rich raw materials are provided for biological synthesis of cucurbitacin such as hemsleyadin and the like.

Disclosed is a method of enhancing the response of cancer cells in a mammal to treatment with a death receptor ligand, which method comprises contacting the cancer cells with a death receptor ligand in conjunction with an effective amount of a compound described herein, for example, a cucurbitacin (I) or a withanolide (II). Also disclosed is a method of inducing apoptosis in cancer cells in a mammal, comprising contacting the cancer cells with a compound described herein, for example, a cucurbitacin (I) or a withanolide (II) and also contacting the cancer cells with a death receptor ligand, whereby apoptosis is induced in the cancer cells.

The invention discloses a method for separating cucurbitacin B from muskmelon vines by virtue of catalytic reaction. The method comprises the following steps: an extraction process, namely taking 1 part of the muskmelon vines, soaking by using ethanol, performing reflux extraction, and filtering and concentrating an extracting solution to obtain an extract; the catalytic reaction, namely adding concentrated hydrochloric acid into the extract, then adding ferric trichloride hexahydrate, stirring and reacting, cooling to the room temperature, filtering, performing water rinsing, and drying; a refining process, namely stirring and dissolving a crude product by using ethyl acetate, then adding activated carbon, decoloring, concentrating to a small volume, and standing overnight to perform crystallization; a recrystallization process, namely dissolving a like fine product by using ethanol, filtering and concentrating to a small volume while hot, standing overnight to perform crystallization, and filtering; and putting the fine product in a vacuum drying box, and drying for 8 hours to obtain a cucurbitacin B fine product. The method disclosed by the invention is wide in raw material source and low in production cost, and can be used for reducing the damages of an acid environment to cucurbitacin B; the production rate reaches more than 60%, the purity is more than 99.0%, and the wastewater BOD (biochemical oxygen demand) is less than 100; the method is suitable for the industrial production of recycling water, and can be widely applied to plant extraction industries.

A pure cucurbitacin product is obtained through conducting grinding, petroleum ether leaching and filtering on siraitia grosvenorii roots, adsorbing by resin after reduced pressure concentration on filtrate, eluting by different concentrations of eluents, decoloring the eluents, crystallizing and recrystallizing, and starch is obtained by jordaning filter residue, filtering to obtain starch milk, precipitating the starch milk through centrifugal separation, and drying or dehydrating 95% of ethanol. The cucurbitacin substances and the starch substances can be obtained at the same time by the method, so that the utilization rate of the siraitia grosvenorii roots is improved greatly, the starch resource is saved, the operation is simple, the residual of cucurbitacins in the starch in siraitia grosvenorii roots is reduced greatly, and the use scope of starch is expanded.

The invention relates to a method for preparing cucurbiturils. The method comprises reacting an oligomer consisting of 2 to 11 linked glycolurils or glycoluril analogues with one or more compounds selected from glycoluril, glycoluril analogues and / or oligomers of glycoluril or glycoluril analogues, in the presence of an acid, to form a cucurbituril. The method can be used to prepare variably substituted cucurbiturils having specific substituted units at specific locations in the cucurbituril. The invention also provides cucurbiturils prepared by the method of the invention. The invention further provides novel cucurbiturils.