Gene cluster participating in synthesis of cucurbitacin E of watermelon and application of gene cluster
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A gene cluster, cucurbitacin technology, applied in the fields of genetic engineering and molecular biology, to achieve the effect of speeding up the breeding process
Inactive Publication Date: 2015-11-11
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
View PDF1 Cites 12 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Cucurbitacin has anti-insect effect, and medical research has proved that cucurbitacin can inhibit the growth of various canc
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0017] Example 1 Mining and acquisition of gene clusters involved in the synthesis of watermelon cucurbitacin E
[0018] 1. Mining of gene clusters
[0019] Based on the research on the synthesis gene cluster of cucumber bitter substance cucurbitacin C, a gene cluster similar to the synthesis of cucumber cucurbitacin C was found in the watermelon genome by using the method of comparative genomics. A total of 8 genes were found, including 1 cucurbitadiene Alcohol synthase gene Bi (Cla007080), 6 P450 genes (Cla007077, Cla007078, Cla007079, Cla007082, Cla008354, and Cla008355) and 1 acetyltransferase gene (Cla007081), except for Cla008354 and Cla008355 genes, other genes are located on chromosome 6 Within the range of 57kb, a structure similar to a gene cluster was formed, and these eight genes were specifically co-expressed in watermelon roots, and were positively correlated with the content of cucurbitacin E in each tissue ( figure 1 ), so that the gene cluster is involved ...
Embodiment 2
[0027]Example 2 Using the yeast expression system to verify the function of the cucurbitacin E synthesis candidate gene
[0028] The Cla007077, Cla007078, Cla007079, Cla007080, Cla007081, Cla007082, Cla008354 and Cla008355 genes and the reductase CPR gene were respectively constructed on the expression vector pYES2 (Invitrogen), and transformed into yeast. Induce the yeast to express the protein and collect the yeast. After cracking, the compound was extracted with petroleum ether. After the samples were prepared, they were detected by UPLC-Q-TOF. The results were Such as figure 2 , image 3 shown.
[0029] figure 2 It is the result of using UPLC-Q-TOF to detect the second and third steps of bitterin synthesis; where A is the specific product of Cla008354 and Cla008355 detected by UPLC-Q-TOF, and B is the MS / MS detection results, C is nuclear magnetic resonance NMR structure analysis results.
[0030] image 3 It is the result of using UPLC-Q-TOF to detect t...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a gene cluster participating in synthesis of cucurbitacin E of watermelon and an application of the gene cluster. According to the invention, the gene cluster participating in synthesis of cucurbitacin E is found in the watermelon genome for the first time, the gene cluster comprises eight genes, wherein six of the eight genes are positioned within the range of the No.6 chromosome 57kb. A yeast system is adopted for analyzing the reaction from the step 1 to the step 4 of the synthesis of cucurbitacin E. The invention further discloses a molecular mechanism for forming the bitter taste of the watermelon, so that a theoretical basis and a molecule assistant breeding objective are provided for the breeding of the watermelon without the bitter taste, and valuable experiences are provided for heterologous biosynthesis and development and utilization of cucurbitacine. The technology provided by the invention can replace the traditional method for extracting bitter principle from a plant material, so that synthesis of the bitter principle in vitro is realized by utilizing synthetic biology.
Description
technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a gene cluster involved in the synthesis of watermelon cucurbitacin E and its application. Background technique [0002] The bitter taste of watermelon is caused by a class of triterpenoids called cucurbitacin E. The accumulation of cucurbitacin E in watermelon fruit will seriously affect the taste and quality of watermelon. The previous research used genomics, molecular biology, biochemistry and other means to reveal the synthesis pathway of cucurbitacin C, a bitter substance in cucumber, involving 9 genes, including 1 oxidative squalene cyclase Bi, 7 cytochrome P450 gene and an acetyltransferase gene, and fully elucidated the four-step reaction by using biochemical methods, which has important guiding significance for the research of other types of cucurbitacin biosynthetic pathways in cucurbitaceae plants, and also for the other three types of cucurbi...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more