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A kind of method extracting starch and cucurbitacin from Luo Han Guo tuber root

A technology of Luo Han Guo and cucurbitacin, applied in the field of extracting starch and cucurbitacin, and extracting amylopectin and cucurbitacin at the same time, can solve the problem that the roots of Luo Han Guo are not really well utilized, so as to increase the scope of use, reduce production costs, The effect of improving the utilization rate

Inactive Publication Date: 2016-07-27
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0027] In summary, the current utilization of Luo Han Guo mainly uses the fruit to extract mogroside V, and mogroside V is used as a sweetener, while the root of Luo Han Guo mainly stays in the analysis of chemical components, and the root of Luo Han Guo has not really been well obtained. use of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Wash the fresh Luo Han Guo tubers with water, take 200kg of tubers and add 200L of water to grind them, leave them at room temperature for 1 hour, add 300L of petroleum ether for extraction, take the upper layer solution to obtain a solution containing petroleum ether, and depressurize the solution containing petroleum ether at 70°C Concentrate to 80L, put the concentrate on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 200L of eluent I with 60% ethanol; concentrate eluent I to 25L, add 25L of acetone solution, crystallization and recrystallization at room temperature overnight, to obtain 3.56kg of pure cucurbitacin; stirring and refining the lower layer material without petroleum ether, and then passing through a 10-mesh sieve to obtain starch milk, washing Luo Han Guo pulp with 200L of water, and 3000-4000 rpm low-speed centrifugation to obtain precipitates, and low-speed drying and dehydration at about 30-40 ...

Embodiment 2

[0051] Wash the fresh Luo Han Guo tubers with water, take 400kg of tubers and add 500L of water to grind them, leave them at room temperature for 1 hour, add 600L of petroleum ether for extraction, take the upper layer solution to obtain a solution containing petroleum ether, and depressurize the solution containing petroleum ether at 70°C Concentrate to 100L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 250L of eluent I with 60% ethanol; concentrate eluent I to 25L, add 25L of acetone solution, crystallization and recrystallization at room temperature overnight, to obtain 7.34kg of pure cucurbitacin; stirring and refining the lower layer material without petroleum ether, and then passing through a 10-mesh sieve to obtain starch milk, washing Luo Han Guo pulp with 600L of water, and 3000-4000 rpm low-speed centrifugation to obtain precipitates, and low-speed drying and dehydration at a...

Embodiment 3

[0053] Wash the fresh Luo Han Guo tubers with water, take 500kg of tubers and add 900L of water to mince them, leave them at room temperature for 1 hour, add 1000L of petroleum ether for extraction, take the upper solution to obtain a solution containing petroleum ether, and depressurize the solution containing petroleum ether at 70°C Concentrate to 120L, put the concentrated solution on NKA-9 macroporous adsorption resin column, use ethanol concentration of 20-60%, gradient elution, collect 250L of eluent I with 60% ethanol; concentrate eluent I to 30L, add 30L of acetone solution, crystallization and recrystallization at room temperature overnight, to obtain pure cucurbitacin; stirring and refining the lower layer material without petroleum ether, and then passing through a 10-mesh sieve to obtain starch milk, washing Luo Han Guo pulp with 900L of water, and passing through 3000- Centrifuge at 4000 rpm at a low speed to obtain a precipitate, then dry and dehydrate at a low sp...

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Abstract

A pure cucurbitacin product is obtained through conducting grinding, petroleum ether leaching and filtering on siraitia grosvenorii roots, adsorbing by resin after reduced pressure concentration on filtrate, eluting by different concentrations of eluents, decoloring the eluents, crystallizing and recrystallizing, and starch is obtained by jordaning filter residue, filtering to obtain starch milk, precipitating the starch milk through centrifugal separation, and drying or dehydrating 95% of ethanol. The cucurbitacin substances and the starch substances can be obtained at the same time by the method, so that the utilization rate of the siraitia grosvenorii roots is improved greatly, the starch resource is saved, the operation is simple, the residual of cucurbitacins in the starch in siraitia grosvenorii roots is reduced greatly, and the use scope of starch is expanded.

Description

technical field [0001] The invention belongs to the field of natural product processing, and relates to a method for extracting starch and cucurbitacin, and more specifically relates to a method for simultaneously extracting amylopectin and cucurbitacin from the roots of Luo Han Guo. Background technique [0002] The traditional Chinese medicine Luo Han Guo is the ripe fruit of Cucurbitaceae Luo Han Guo. It is a precious specialty and traditional export medicinal material in my country. It has been reported in the literature that it contains three mushroom saponins and flavonoid glycosides. Its sweet ingredients are ideal natural sweeteners. Luo Han Guo has a large root, bitter taste, and slightly cold nature. It has the effects of clearing away heat and dampness, dredging collaterals and relieving pain. It is a commonly used medicinal material in Guangxi. Pharmacological experiments show that the extract of Luo Han Guo root has anti-inflammatory, analgesic, antispasmodic, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B30/00C07J9/00
Inventor 饶力群
Owner HUNAN AGRICULTURAL UNIV
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