Gene cluster participating in synthesis of cucumber cucurbitacine C and application thereof

A gene cluster and cucurbitacin technology, applied in the fields of genetic engineering and molecular biology, can solve the problems that the synthetic gene has not been cloned, the synthetic route is not clear, and cannot be directly synthesized in vitro, so as to speed up the breeding process
CN103483435AActive Publication Date: 2014-01-01INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Publication Date
2014-01-01

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Abstract

The invention discovers a gene cluster participating in synthesis of cucurbitacine C in a cucumber genome for the first time. The gene cluster totally consists of 8 genes, wherein 5 genes are located in a 35kb range of No. 6 chromosome. The 1st to 4th steps of reaction for synthesizing cucurbitacine C are analyzed in a yeast system, and the 8 genes are co-expressed in a tobacco system, so that synthesis of cucurbitacine C can be realized. The invention further discloses a molecular mechanism of cucumber bitter formation, which provides a theoretical basis and a molecular aided breeding goal for breeding bitter-free cucumber; meanwhile, precious experience is provided for in-vitro artificial synthesis of cucurbitacine.
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Description

technical field

[0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a gene cluster involved in the synthesis of cucumber cucurbitacin C and its application. Background technique

[0002] The bitterness of cucumbers is caused by a class of triterpenoids called cucurbitacin C. The accumulation of cucurbitacin C in cucumber fruit will seriously affect the taste and quality of cucumber. Early classical genetic experiments found that the bitter taste of cucumber is controlled by two genes, Bi and Bt. It is found in CN103013966 that the Bi gene encodes oxidized squalene cyclase, which catalyzes the formation of cucurbitacin 2-enol, which is the first step and the most critical step in the synthesis of cucurbitacin C. The synthesis of cucurbitacin C requires a series of catalytic modifications in addition to the first step to generate cucurbitacin 2-enol. However, how cucurbitacin 2-enol is modified in plants to finally synthe...

Claims

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