46 results about "Withanolide" patented technology

Cyclooxygenase-2 enzyme inhibiting withanolides are described. In particular, compounds from Withania somnifera are the preferred source of the withanolides, although they can be from other plant sources. The COX-2 inhibition is selective over COX-1.

The present invention provides a method of treatment or management of various adaptogenic conditions, such as, stress in mammals, more particularly, humans, comprising administering Withania somnifera plant extract. A high purity extract composition comprising withanolide glycosides, oligosaccharides, withanolide aglycones and a minimum level of polysaccharides, and a pharmaceutically, veterinary or nutritionally acceptable carrier(s) is disclosed. Preferably, the composition of the present invention can be devoid of any alkaloids or contains trace levels of alkaloids. The method of treatment or management of stress administering the composition comprising Withania somnifera of the present invention does not suffer from any one of the abovementioned side effects even after prolonged use. A method of preconditioning a mammalian patient to improve the patient's resistance and reaction to subsequently encountered stress is described.

Novel isolated and purified withanamides and withanolides are described. In particular, compounds from Withania somnifera fruit are the preferred source of the withanamides and withanolides, although they can be from other plant sources. In addition to their use as powerful antioxidants, the withanamides and withanolides can be useful for the treatment of depression, Alzheimer's Disease, obesity and migraine headaches.

The invention discloses application of a withanolide compound in preparation of a drug or a healthcare product for treating or preventing tumors and neurodegenerative diseases. The withanolide compound has structural formulas as shown in formula I and optical isomers thereof. The research indicates that the withanolide compounds can be used for activating an Nrf2 signal channel, up regulating expression of NrF2 and downstream antioxidant and II-phase detoxifying enzymes thereof, strengthening the level of glutathione in cells, increasing reducing capacity in cells, promoting elimination of active carbon, restraining lipid peroxidation and lowering cytotoxicity caused by cancerogen arsenic and nerve cell toxicity induced by active oxygen H2O2 to show good chemical prevention effect. And therefore, the withanolide compound and nightshade extract including the withanolide compound have the potential of becoming a chemical prophylactic drug and the healthcare product, and can be used for preparing the corresponding oral preparation and injection by being used as the main ingredient, and also can be used for preventing and treating tumors and neurodegenerative diseases.

Cyclooxygenase-2 enzyme inhibiting withanolides are described. In particular, compounds from Withania somnifera are the preferred source of the withanolides, although they can be from other plant sources. The COX-2 inhibition is selective over COX-1.

Novel isolated and purified withanamides and withanolides are described. In particular, compounds from Withania somnifera fruit are the preferred source of the withanamides and withanolides, although they can be from other plant sources. In addition to their use as powerful antioxidants, the withanamides and withanolides can be useful for the treatment of depression, Alzheimer's Disease, obesity and migraine headaches.

The present invention provides compositions and methods related to enhancing energy levels in an individual. The invention also provides methods for reducing the effects of stress in an individual. Certain embodiments of this invention include a composition comprising niacin bound chromium, D-ribose, a withanolide and an amine selected from the group consisting of phenylalanine, ceffeine, taurine and gltaumine for enhancing energy levels and / or reducing stress in an individual.

Acidic fluids used in wells or pipelines cause corrosion of carbon steel. Methods for inhibiting corrosion include contacting carbon steel with a fluid having an aqueous acidic phase including a material selected from the group consisting of: a material of a plant in the Solanaceae family, an extract of a material of a plant in the Solanaceae family, a withanolide, a source of a withanolide, a withanolide derivative, a source of a withanolide derivative, and any combination thereof. The methods have wide application in various kinds of operations involved in the production or transportation of oil and gas, such as acid stimulation in a well or remedial treatment in a pipeline.

The present invention provides a Withania somnifera fraction rich in withanolides and a vaccine comprising a “Withania somnifera fraction” as an adjuvant.

The invention discloses a method for extracting withanolide. The method has the characteristics of high efficiency, little pollution and simple operation, and the steps are as follows: a. Take the raw material of Indian ginseng root and grind it into 40-80 mesh, put it in the extraction kettle, at the extraction pressure of 22-27MPa, and the temperature of 40-50°C, put in liquid CO2 and entrainer, the flow rate is 20-40L / h, and extract 2-3 After 1 hour, analyze the extract; b. Add water to disperse the recovery reagent of the above extract, add it to the macroporous resin for separation, ethanol solution gradient elution, the eluate is concentrated to a small volume of crystals, recrystallized with ethanol for 2-3 times, and dried Get withanide.

Novel withanolide chemical genetic probes identify the in vivo binding target of withaferin A, which is the intermediate filament type III protein vimentin. In addition, a withanolide-based small molecule screening method screens drug candidates that target intermediate filament type III proteins. The method includes introducing a tagged linker covalently bonded to the withanolide molecule to form a withanolide probe. Better or alternative small molecule compounds as potential drug candidates can be generated based on their likely affinity for the determined binding site in vimentin. The affinity labeled withanolide can also be used to find intermediate filament-associated proteins using chemical proteomics by extracting proteins from cells that were exposed to withanolide-biotin analog. The withanolide probes can be used to monitor expression of vimentin, in tumor samples or other diseased tissues. Withaferin analogs can be used as a treatment for diverse vimentin-associated disorders, such as cancers, angiofibrotic diseases, and chronic inflammation.

The present invention provides a Withania somnifera fraction rich in withanolides and a vaccine comprising a “Withania somnifera fraction” as an adjuvant.

A method of treating tinnitus includes the step administering a composition to the mammal, wherein the composition consists essentially of a biologically effective amount of vitamin A, vitamin E, vitamin C, a vasodilator comprising magnesium, and, optionally, a withanolide, and / or resveratrol.

The invention discloses seven withanolides compounds derived from cape gooseberries as well as a preparation method and application of the withanolides compounds, and relates to structures of five new(1-5) and two known (6-7) withanolides compounds in the cape gooseberries, a preparation method of the withanolides compounds and application of the withanolides compounds in anti-inflammatory and neurodegenerative disease treatment medicines. The compounds (2-4, 6, 7) provided by the invention have good NO inhibition activity, and can be used for development and application of anti-inflammatoryand neurodegenerative disease treatment drugs.

A composition to be administered to a mammal for treating hearing loss is disclosed herein. The composition consists essentially of a biologically effective amount of vitamin A, vitamin C, vitamin E, and a vasodilator comprising magnesium. The composition optionally includes a withanolide and / or resveratrol. The vitamin A, vitamin C, vitamin E, and the vasodilator comprising magnesium provides an additive effect that is equal to or greater than a sum of the effects of the individual components.

Provided herein are synthetic analogs of withanolide natural products of formula (I), wherein R1-R4 are as defined herein, and their pharmaceutical uses in treating neurodegenerative diseases.

Disclosed is a method of enhancing the response of cancer cells in a mammal to treatment with a death receptor ligand, which method comprises contacting the cancer cells with a death receptor ligand in conjunction with an effective amount of a compound described herein, for example, a cucurbitacin (I) or a withanolide (II). Also disclosed is a method of inducing apoptosis in cancer cells in a mammal, comprising contacting the cancer cells with a compound described herein, for example, a cucurbitacin (I) or a withanolide (II) and also contacting the cancer cells with a death receptor ligand, whereby apoptosis is induced in the cancer cells.

A method of treating hearing loss includes the step administering a composition to the mammal, wherein the composition consists essentially of a biologically effective amount of vitamin A, vitamin E, vitamin C, a vasodilator comprising magnesium, and, optionally, a withanolide, and / or resveratrol.

A method for prevention and / or treatment of diminution of bone tissue using a composition comprising a compound selected from Withaferin A (WFA), Withanolide A, and Withanone. The composition showed enhanced WFA bioavailability in rodents against plain WFA, promotes bone marrow cell differentiation, and increases the percent of bone volume to tissue volume (BV / TV%) by 3 folds as compared to free Withaferin A (WFA).

A method for treating noise-induced hearing loss (NIHL) includes the step administering a composition to the mammal, wherein the composition consists essentially of a biologically effective amount of vitamin A, vitamin E, vitamin C, a vasodilator comprising magnesium, and, optionally, a withanolide, and / or resveratrol.

The invention discloses a withanolide compound and application thereof. The withanolides compound is characterized in that the withanolides compound has a structure as shown in a formula (I) or an optical isomer thereof, activity evaluation is carried out by using an in-vitro activity screening evaluation system, the compound disclosed by the invention is found to be capable of effectively inhibiting the growth of human malignant melanoma cells, and the withanolides compound disclosed by the invention is prompted to be capable of being used as a medicine for treating tumor diseases.

The invention provides to an enriched Withania somnifera (Ashwagandha) extract composition comprising withanolide glycosides, withanolide aglycones and reduced levels of withaferin-A. The invention also provides to process for the preparation of these compositions and further provides to methods of improving testosterone levels, energy levels, sustained energy, vigor, stamina, and muscle mass and muscle strength using these compositions.

The invention discloses a method for extracting enriched withanolides total steroids from waste lycium chinensis branches. The method includes the steps of (1) extracting the waste lycium chinensis branches by ethyl alcohol, blending extracting solutions and recovering the ethyl alcohol so as to obtain a concentrated solution; (2) adding ethyl acetate into the concentrated solution to extract, and recovering organic phases prior to concentrating to obtain extract; (3) diluting the extract, then adopting a selective macroporous resin column which is eluted by the ethyl alcohol, recovering the ethyl alcohol from obtained eluent and crystallizing by the ethyl alcohol so as to obtain the withanolides total steroids. The method is simple and feasible in technology and high in extraction yield, thereby being suitable for industrial production.

The invention discloses a method for extraction separation of a withanolides compound from waste Chinese wolfberry branches. The method comprises the steps of: (1) extracting waste Chinese wolfberry branches with ethanol, combining the extracted liquid, and recovering ethanol to obtain a concentrated solution; (2) adding ethyl acetate into the concentrated solution to perform extraction, recovering the organic phase and then performing concentration to obtain an extract; (3) diluting the extract, loading the diluted extract to a selective macroporous adsorption resin column, conducting elution with ethanol, recovering ethanol from the obtained eluent, and conducting crystallization with ethanol so as to obtain the withanolides compound; (4) loading the withanolides compound crystal to a silica gel column, performing elution with a mixed solution of n-hexane and ethyl acetate, carrying out segmented collection of the eluent, thus obtaining withanolides A and withanolides B respectively in order; and (5) recovering the solvent from the segmented eluent in step (4), and then performing crystallization with ethanol so as to obtain a withanolides A monomer and a withanolides B monomer respectively. The method provided by the invention has high extraction yield, and the obtained monomers have high purity.

A method for treating age-related hearing loss (ARHL) includes the step administering a composition to the mammal, wherein the composition consists essentially of a biologically effective amount of vitamin A, vitamin E, vitamin C, a vasodilator comprising magnesium, and, optionally, a withanolide, and / or resveratrol.