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Method for extracting enriched withanolides total steroids from waste lycium chinensis branches

A technology of ashwagandha lactone and total steroids is applied in the field of extraction technology of active components of lycium barbarum, which can solve the problems of few reports on basic research and applied research of lycium barbarum branches, waste of resources and the like, and achieves high product yield and reasonable process route. Effect

Inactive Publication Date: 2016-02-10
QINGHAI TIBET BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the basic research and applied research on the Lycium barbarum branch that grows with a Lycium barbarum
Especially in spring every year, in order to increase the yield, the farmer prunes wolfberry, and a large number of waste wolfberry branches are piled up on the roadside ridges. When the garbage is disposed of, it is a great waste of resources for the arid regions of Northwest my country.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method embodiment of the present invention for extracting and enriching withanolide total steroids from the waste branches of Lycium barbarum, comprising the following steps:

[0022] (1) Take 10 kg of wolfberry waste branches, use 100 liters of ethanol with a volume concentration of 80% to extract the waste branches of wolfberry at 50°C for 1 hour, and separate the waste branches of wolfberry with a 200-mesh filter cloth; then use 60 liters of ethanol with a volume concentration of Extract 80% ethanol at 70°C for 1 hour, and separate the waste branches of Lycium barbarum with a 200-mesh filter cloth; then use 50 liters of ethanol with a volume concentration of 80% to extract 1 hour of the waste branches of Lycium barbarum at 50°C. hour, with 200 mesh filter cloths, the waste branches of Lycium barbarum are separated; the extracts obtained from the three extractions are combined, ethanol is reclaimed, and the extracts are concentrated to 100 liters of concentrates;

...

Embodiment 2

[0026] A method embodiment of the present invention for extracting and enriching withanolide total steroids from the waste branches of Lycium barbarum, comprising the following steps:

[0027] (1) Take 50 kg of waste wolfberry branches, use 400 liters of ethanol with a volume concentration of 60% to extract the waste branches of wolfberry at 70°C for 3 hours, and separate the waste branches of wolfberry with a 200-mesh filter cloth; then use 400 liters of ethanol with a volume concentration of Extract 60% ethanol at 70°C for 3 hours, and use a 200-mesh filter cloth to separate the waste branches of Lycium barbarum; then use 300 liters of ethanol with a volume concentration of 60% to extract the waste branches of Lycium barbarum for 3 hours at 70°C hours, with 200 mesh filter cloths, the waste branches of Lycium barbarum are separated; the extracts obtained from three extractions are combined, ethanol is recovered, and the extracts are concentrated to 500 liters of concentrates;...

Embodiment 3

[0031] A method embodiment of the present invention for extracting and enriching withanolide total steroids from the waste branches of Lycium barbarum, comprising the following steps:

[0032] (1) Take 40 kg of wolfberry waste branches, use 280 liters of ethanol with a volume concentration of 70% to extract the waste branches of wolfberry at 70°C for 1 hour, and separate the waste branches of wolfberry with 200 mesh filter cloth; then use 280 liters of ethanol with a volume concentration of Extract 70% ethanol at 60°C for 2 hours, and separate the waste branches of Lycium barbarum with a 200-mesh filter cloth; then use 280 liters of ethanol with a volume concentration of 70% to extract 2 hours at 70°C for the waste branches of Lycium barbarum hour, with 200 mesh filter cloths, the waste branches of Lycium barbarum are separated; the extracts obtained from three extractions are combined, ethanol is reclaimed, and the extracts are concentrated to 400 liters of concentrates;

[0...

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PUM

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Abstract

The invention discloses a method for extracting enriched withanolides total steroids from waste lycium chinensis branches. The method includes the steps of (1) extracting the waste lycium chinensis branches by ethyl alcohol, blending extracting solutions and recovering the ethyl alcohol so as to obtain a concentrated solution; (2) adding ethyl acetate into the concentrated solution to extract, and recovering organic phases prior to concentrating to obtain extract; (3) diluting the extract, then adopting a selective macroporous resin column which is eluted by the ethyl alcohol, recovering the ethyl alcohol from obtained eluent and crystallizing by the ethyl alcohol so as to obtain the withanolides total steroids. The method is simple and feasible in technology and high in extraction yield, thereby being suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of extracting active components of wolfberry, and in particular relates to a method for extracting and enriching total steroids of withanolalide from waste branches of wolfberry. Background technique [0002] Withanalides are a class of naturally occurring ergostane 26 carboxylic acid lactones, which are the earliest steroids from natural sources. As early as the 1960s and 1970s, it has been found that these compounds have obvious biological activities, especially in antibacterial, anti-inflammatory, cytotoxic, cellular immunity and anti-tumor aspects. [0003] Withanolide compounds are basically distributed in some genera of Solanaceae, mainly including the genus Solanum (Solanum), followed by the genus Physalis, Datura, and Lycium barbarum. The plants of the genus Ashwagandha are widely distributed in South Africa, South America, West Asia, Northeast Atlantic and other places, and are especially widely us...

Claims

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Application Information

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IPC IPC(8): A61K36/815
CPCA61K36/815A61K2236/33A61K2236/333A61K2236/39A61K2236/55
Inventor 陈宝华
Owner QINGHAI TIBET BIOTECH CO LTD
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