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Method for extraction separation of withanolides compound from waste Chinese wolfberry branches

A technology for ester compounds and ashwagandha lactones, which is applied in the field of extraction and separation of ashwagandha lactone compounds, can solve the problems of few reports on basic research and applied research, waste of resources and the like, and achieves high product yield and reasonable process route. Effect

Inactive Publication Date: 2016-04-20
赵军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the basic research and applied research on Ningxia Lycium barbarum branch that grows with a single branch of Lycium barbarum Ningxia
Especially in spring every year, in order to increase the yield, the farmer prunes wolfberry, and a large number of waste wolfberry branches are piled up on the roadside ridges. When the garbage is disposed of, it is a great waste of resources for the arid regions of Northwest my country.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The present invention extracts and separates the method embodiment of the withanolalide compound from the waste branches of Lycium barbarum, which comprises the following steps:

[0026] (1) Get 10 kilograms of wolfberry waste branches, use 100 liters of ethanol with a volume concentration of 80% to extract the wolfberry waste branches for 1 hour at 50 ° C, and separate the wolfberry waste branches with 200 mesh filter cloth; then use 60 liters of volume concentration Extract 80% ethanol at 70°C for 1 hour, and use a 200-mesh filter cloth to separate the waste branches of Lycium barbarum; then use 50 liters of ethanol with a volume concentration of 80% to extract 1 hour of the waste branches of Lycium barbarum at 50°C. hour, with 200 mesh filter cloths, the waste branches of Lycium barbarum are separated; the extracts obtained from the three extractions are combined, ethanol is reclaimed, and the extracts are concentrated to 100 liters of concentrates;

[0027] (2) Add ...

Embodiment 2

[0032] The present invention extracts and separates the method embodiment of the withanolalide compound from the waste branches of Lycium barbarum, which comprises the following steps:

[0033] (1) Get 50 kilograms of wolfberry waste branches, use 400 liters of ethanol with a volume concentration of 60% to extract the wolfberry waste branches for 3 hours at 70 ° C, and separate the wolfberry waste branches with 200 mesh filter cloth; then use 400 liters of volume concentration Extract 60% ethanol at 70°C for 3 hours to extract the waste branches of Lycium barbarum, separate the waste branches of Lycium barbarum with 200 mesh filter cloth; then use 300 liters of ethanol with a volume concentration of 60% to extract the waste branches of Lycium barbarum for 3 hours at 70°C hours, with 200 mesh filter cloths, the waste branches of Lycium barbarum are separated; the extracts obtained from three extractions are combined, ethanol is recovered, and the extracts are concentrated to 500...

Embodiment 3

[0039] The present invention extracts and separates the method embodiment of the withanolalide compound from the waste branches of Lycium barbarum, which comprises the following steps:

[0040] (1) Get 40 kilograms of wolfberry waste branches, use 280 liters of ethanol with a volume concentration of 70% to extract the wolfberry waste branches for 1 hour at 70 ° C, and separate the wolfberry waste branches with 200 mesh filter cloth; then use 280 liters of volume concentration Extract 70% ethanol at 60°C for 2 hours, and separate the waste branches of Lycium barbarum with a 200-mesh filter cloth; then use 280 liters of ethanol with a volume concentration of 70% to extract 2 hours at 70°C for the waste branches of Lycium barbarum hour, with 200 mesh filter cloths, the waste branches of Lycium barbarum are separated; the extracts obtained from three extractions are combined, ethanol is reclaimed, and the extracts are concentrated to 400 liters of concentrates;

[0041] (2) Add 40...

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Abstract

The invention discloses a method for extraction separation of a withanolides compound from waste Chinese wolfberry branches. The method comprises the steps of: (1) extracting waste Chinese wolfberry branches with ethanol, combining the extracted liquid, and recovering ethanol to obtain a concentrated solution; (2) adding ethyl acetate into the concentrated solution to perform extraction, recovering the organic phase and then performing concentration to obtain an extract; (3) diluting the extract, loading the diluted extract to a selective macroporous adsorption resin column, conducting elution with ethanol, recovering ethanol from the obtained eluent, and conducting crystallization with ethanol so as to obtain the withanolides compound; (4) loading the withanolides compound crystal to a silica gel column, performing elution with a mixed solution of n-hexane and ethyl acetate, carrying out segmented collection of the eluent, thus obtaining withanolides A and withanolides B respectively in order; and (5) recovering the solvent from the segmented eluent in step (4), and then performing crystallization with ethanol so as to obtain a withanolides A monomer and a withanolides B monomer respectively. The method provided by the invention has high extraction yield, and the obtained monomers have high purity.

Description

technical field [0001] The invention relates to a method for extracting and separating withanolide compounds, in particular to a method for extracting and separating withanolalide compounds from waste branches of wolfberry. Background technique [0002] Withanalides are a class of naturally occurring ergostane 26 carboxylic acid lactones, which are the earliest steroids from natural sources. As early as the 1960s and 1970s, it has been found that these compounds have obvious biological activities, especially in antibacterial, anti-inflammatory, cytotoxic, cellular immunity and anti-tumor aspects. [0003] Withanolide compounds are basically distributed in some genera of Solanaceae, mainly including the genus Solanum (Solanum), followed by the genus Physalis, Datura, and Lycium barbarum. The plants of the genus Ashwagandha are widely distributed in South Africa, South America, West Asia, Northeast Atlantic and other places, and are especially widely used in Indian Ayurvedic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
Inventor 赵军陈宝华
Owner 赵军
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