Extraction and preparation method of small molecular fish skin collagen peptide

A technology of fish skin collagen and small molecules, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of wide molecular weight distribution, high price of protease, and limited application range, etc., and achieve good application Value and market potential, the effect of increasing the speed of enzymatic hydrolysis, and being easy to produce on an industrial scale

Inactive Publication Date: 2019-03-19
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of protease, if the extraction process is not properly designed, not only a large amount of protease needs to be added, but also the enzymatic hydrolysis time is long, resulting in high produc

Method used

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Examples

Experimental program
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Effect test

Example Embodiment

[0034] Example 1

[0035] A method for extracting and preparing small molecule fish skin collagen peptides specifically includes the following steps:

[0036] S1. Preparation of raw materials: take fresh fish to collect fish skin, wash the fish skin, drain the water on the surface of the fish skin, and chop the fish skin;

[0037] S2, degreasing treatment: add sodium hydroxide and water to the chopped fish skin, stir for 20 minutes and then stand for 4 hours, filter out the water, and degrease the fish skin. After degreasing, rinse the fish skin twice; in which sodium hydroxide The amount of water added is 1% of the quality of fish skin, and the amount of water added is twice the quality of fish skin.

[0038] S3. Enzymatic hydrolysis pretreatment: add deionized water of 2 times the mass of the crushed fish skin to the defatted crushed fish skin, beat and mix to obtain a slurry;

[0039] S4. Enzymatic hydrolysis treatment: first add 0.1% flavour protease by weight of the slurry, adjust...

Example Embodiment

[0048] Example 2

[0049] A method for extracting and preparing small molecule fish skin collagen peptides specifically includes the following steps:

[0050] S1. Preparation of raw materials: take fresh fish to collect fish skin, wash the fish skin, drain the water on the surface of the fish skin, and chop the fish skin;

[0051] S2, degreasing treatment: add sodium hydroxide and water to the chopped fish skin, stir for 20 minutes and then let it stand for 4.5 hours, filter out the water, and degrease the fish skin. After degreasing, rinse the fish skin twice; among them, the water is oxidized. The amount of sodium added is 1.2% of the mass of fish skin, and the amount of water added is 2.5 times the mass of fish skin.

[0052] S3. Enzymatic hydrolysis pretreatment: add 2.5 times the mass of deionized water to the defatted fish skin, beat and mix to obtain a slurry;

[0053] S4. Enzymatic hydrolysis treatment: first add flavor protease of 0.12% of the slurry weight, adjust the pH to 6...

Example Embodiment

[0062] Example 3

[0063] A method for extracting and preparing small molecule fish skin collagen peptides specifically includes the following steps:

[0064] S1. Preparation of raw materials: take fresh fish to collect fish skin, wash the fish skin, drain the water on the surface of the fish skin, and chop the fish skin;

[0065] S2, degreasing treatment: add sodium hydroxide and water to the chopped fish skin, stir for 25 minutes and let stand for 5 hours, filter out the water, and degrease the fish skin. After degreasing, rinse the fish skin 3 times; of which sodium hydroxide The amount of water added is 1.5% of the quality of the fish skin, and the amount of water added is 3 times the quality of the fish skin.

[0066] S3. Enzymatic hydrolysis pretreatment: add deionized water 5 times the mass of the defatted fish skin to the defatted fish skin, beat and mix to obtain a slurry;

[0067] S4. Enzymatic hydrolysis treatment: firstly add 0.15% flavour protease by weight of the slurry, ...

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Abstract

The invention discloses an extraction and preparation method of small molecular fish skin collagen peptide. The extraction and preparation method comprises the following steps: preparing raw materials, carrying out enzymolysis pre-treatment, carrying out enzymolysis treatment and the like. According to the extraction and preparation method of the small molecular fish skin collagen peptide, provided by the invention, fish skin is firstly subjected to degreasing treatment and then is subjected to the enzymolysis treatment; and in an enzymolysis treatment process, various types of different proteinases are used for carrying out enzymolysis on the fish skin, so that the enzymolysis speed can be improved, the dosage of the proteinase also can be reduced, and the yield of the small molecular fish skin collagen peptide can be effectively improved. According to the extraction method provided by the invention, modified activated carbon is used for de-coloring, the adsorption performance is excellent and enzymolysis liquid can be uniformly distributed; good de-coloring and deodorization effects can be realized under the conditions of small dosage and short time and effective components are not lost; and the extraction method has the advantages of low investment, simplicity in operation, good protein degradation effect and high yield of the fish skin collagen peptide, the industrial large-scale production is easy to realize and the extraction method has good application value and market potential.

Description

technical field [0001] The invention relates to the technical field of extracting collagen peptides, in particular to a method for extracting and preparing small molecule fish skin collagen peptides. Background technique [0002] Collagen is a natural biopolymer synthesized by animal cells. As an important component of the extracellular matrix, it exists in almost all tissues, accounting for about 30% of the total animal protein. Collagen has a high ability to resist extension, making skin and other connective tissue Tissues can maintain a certain structure and have special mechanical properties to support and protect cells, tissues and body functions, which is critical to the structure and performance of cells and tissues. [0003] Collagen peptide refers to a low-molecular-weight biologically active skin with special functions obtained from collagen tissue through deep hydrolysis. Compared with collagen, collagen peptide is more easily absorbed by the human body, and the a...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCC07K14/78C12P21/06
Inventor 洪鹏志刘唤明周春霞邓楚津杨萍
Owner GUANGDONG OCEAN UNIVERSITY
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