Sucrose-DNA compound, its preparation method, related kit and method for detecting different HBV types by cooperating with glucometer

Abstract

The invention discloses a sucrose-DNA compound, one end of its molecular is amino-containing sucrose, and the other end is an auxiliary probe hybridisable on different HBV genotypes. The sucrose and the auxiliary probe are connected by a connecting arm, which includes a 4-(N-maleimidomethyl)cyclohexane group. The kit provided in the invention comprises a microfluidic chip and a reporter probe solution. The chip is internally provided with a main channel and a plurality of side channels, and the different side channels are internally fixed with capture probes directed at different HBV types. The report probe solution is prepared from the compound. The invention also discloses a preparation method of the compound and the kit. The kit can cooperate with a glucometer to detect different HBV types. During detection, a target gene is first hybridized, then the report probe is hybridized, and finally a substrate is introduced to cooperate with a glucometer detection signal to make judgment. The kit disclosed in the invention has the advantages of low cost, easy operation, no need of foreign aid equipment, convenient use and carry, and accurate detection result.

Description

Technical field [0001] The present invention relates to an enzyme-DNA-binding complex and its preparation and application in virus detection, in particular to a sucrase-DNA complex and a preparation method thereof, and a kit made of the complex and its application in virus detection. The specific application of different genotyping tests of hepatitis B virus. Background technique [0002] Viral hepatitis B is an infectious disease that seriously harms human health. There are 350 million hepatitis B virus carriers in the world. Hepatitis B not only harms human health, but also imposes a heavy economic burden on families and society. Hepatitis B virus (HBV) is the smallest DNA virus currently known, with a partially double-stranded circular genome with a full length of 3.2Kb. HBV has 8 genotypes, and there are differences in pathogenicity and drug resistance between different genotypes. Since more than 95% of hepatitis B virus carriers in China can be classified into the three HB...

AI Technical Summary

Problems solved by technology

However, the existing portable detection instruments can only detect single target

In recent years, some researchers have developed a new method of combining portable blood glucose meters with functionalized DNA sensors to detect and analyze other targets other than blood sugar, such as Lu et al. (Yu Xiang, Yi Lu. Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets. Nature Chemistry, 2011, 3(9): 697-703.), Yuan et al. (Jiao Su, Jin Xu. Ying Chen. et al. Personal glucose sensor for point-of -care early cancer diagnosis.Chem.Commun.2012, 48, 6909-6911) using a blood glucose meter to detect individual small molecules, proteins, and DNA, but there is currently no use of a portable blood glucose meter to detect hepatitis B virus gene analysis. type detection technology

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