Method for identifying varieties of low-phenol cotton

Abstract

The invention discloses a method for identifying the varieties of low-phenol cotton. The genome DNA of each tested material is extracted; three SSR (simple sequence repeats) primers are respectively selected on 26 linkage groups of the cotton, so that the genome DNA of each tested material is used as a template for PCR (polymerase chain reaction) amplification; the PCR amplification result is analyzed; a fingerprint atlas of each variety of the low-phenol cotton is built; different varieties of feature primers are screened out; 53 pairs of primers express stable polymorphism among different tested materials; at least two pairs of feature primers are used and combined, and the varieties of the low-phenol cotton can be distinguished; the powerful technical support is provided for the identification, the development and the protection of the low-phenol cotton in future.

Description

technical field [0001] The invention relates to a method for identifying low-phenol cotton varieties, belonging to the technical field of molecular biology. Background technique [0002] Cotton is the main economic crop in my country, which integrates fiber, protein and oil, and cotton fiber has been fully utilized; protein and oil come from cottonseed, which contains 22% of oil in addition to 18%-20% of oil -24% protein. The protein content of cottonseed flour after cottonseed oil extraction can be as high as 45%-50%, which is far higher than that of rice, wheat, peanuts, and soybeans, and contains some essential nutrients that are lacking in wheat, rice, and soybeans. essential amino acids. Cottonseed also contains more than 35% cottonseed oil. Among edible oils, cottonseed oil has the highest linoleic acid content. The linoleic acid content of cottonseed oil is as high as 55.6%, which is a high-grade health care oil with extremely high linoleic acid content. Therefore,...

AI Technical Summary

Problems solved by technology

The content of gossypol in the seeds of low-phenol cotton reaches the national food standard (<0.02%) and the international foo

Images