Analytical method for structures of microbial communities in composite filler in tower-type earthworm ecological filter

A microbial community and ecological filter technology, applied in the field of environmental microbial molecular ecology, can solve the problems of a wide variety of microorganisms, complex ecological filter fillings, and less research

Inactive Publication Date: 2012-07-11
郭飞宏
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are patent applications for the use of DGGE technology to analyze microbial communities in sewage plant sludge and industrial wastewater treatment, but the tower-type earthworm ecological filter used to treat rural domestic sewage has complex fillers and a wide variety of microorganisms, so there are few studies in this area

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Analytical method for structures of microbial communities in composite filler in tower-type earthworm ecological filter
  • Analytical method for structures of microbial communities in composite filler in tower-type earthworm ecological filter
  • Analytical method for structures of microbial communities in composite filler in tower-type earthworm ecological filter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] 1. Packing sample collection and pretreatment

[0027] Use a 10cm x 8cm sterile belt to collect about 50g of soil (10cm), sandstone (5cm) and crushed bluestone (3cm) fillers in the first, second and third stages of the tower earthworm ecological filter, and store them at -80°C after collection. Freeze-dried for 5 days. The freeze-dried filler samples were ground with a sterilized mortar, and then plugged with a 100-mesh stopper. After the plug was plugged, they were packed into new sterile belts and refrigerated at -20°C.

[0028] 2. DNA extraction and purification

[0029] Weigh 0.5g of the pretreated filler sample and add it to Bead Solution Tubes, then add 60ul of Solution S1 and 200ul of IRS solution and vortex for 15min on a vortex machine; transfer the supernatant from 10000g centrifugation for 30s to 2mlCollection Tubes, add 250ul of Solution Vortex S2 for 5s and then stand at 4°C for 5min; centrifuge at 10000g for 1min, transfer the supernatant to 2mlCollectio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an analytical method for compositions of microbial communities in composite fillers (soil, gravels and broken bluestones) in a tower-type earthworm ecological filter. The analytical method can be used for better reflecting the diversity and dynamic variation of microbial populations and specifically comprises the following steps of: (1). respectively taking samples in different filler layers of a tower-type earthworm ecological filter for preprocessing; (2) extracting the total DNAs (Deoxyribose Nucleic Acids) of genomes by using a MOBIO DNA extraction kit; (3) carrying out nest type PCR (Polymerase Chain Reaction) amplification on the DNAs of the samples by using a design primer of a 16S RNA V3 region; (4) analyzing PCR amplification products by using a denaturing gradient gel electrophoresis (DGGE) technology, and analyzing an obtained DGGE atlas by using Quantity One software; (5) cutting and recycling characteristic DGGE bands, carrying out sequencing analysis on the DNAs of the samples after carrying out PCR amplification again, and judging the variety of microorganisms.

Description

technical field [0001] The invention relates to the use of DNA extraction kits and PCR-DGGE technology to analyze and detect the composition of microbial communities in multi-stage composite fillers (soil, sand and gravel) in tower-type earthworm ecological filters, belonging to environmental microbial molecules field of ecology. Background technique [0002] The tower type earthworm ecological filter is a kind of biological and ecological combination technology designed according to the ecological function of earthworms to improve soil water permeability and promote the decomposition and transformation of organic matter, because of its strong ability to remove nitrogen and phosphorus, low operating costs, It has the advantages of no sludge generation and is widely used in the field of rural domestic sewage treatment. In order to improve the performance and stability of wastewater treatment, a lot of research has been done on the operating conditions and pollutant removal m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04G01N27/447
Inventor 郭飞宏郭敏汪龙眠王正芳罗艳
Owner 郭飞宏
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap