Multiple PCR method for detecting quinolone antibiotics campylobacter jejuni and kit

Abstract

The invention integrates mispairing amplification mutation analysis PCR (MAMA-PCR) and a multiple PCR technique, and provides a method for rapidly detecting quinolone antibiotics campylobacter jejuni caused by point mutation of nucleotide and a kit. The kit comprises a primer composition with the nucleotide sequence shown by SEQ ID No.1-5 and SEQ ID No.7 in the sequence list and a quality control group primer composition with the nucleotide sequence shown by SEQ ID No.1-6 in the sequence list. By adopting the detection method and the kit, campylobacter jejuni of the quinolone antibiotics can be rapidly identified from suspected bacteria, and complex procedures of the traditional detection method using strain identification and drug sensitive test as the representative are simplified. The method and the kit have favorable specificity and sensitivity, and are very suitable for rapidly detecting suspected bacteria at the frontline to provide accurate medicine instructions.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular biology technique for rapidly detecting quinolone-resistant antibiotic Campylobacter jejuni. Background technique [0002] Campylobacter jejuni is a major food-borne infectious pathogen, and diarrhea caused by Campylobacter jejuni infection ranks first among bacterial diarrheal diseases in many economically developed countries. In developing countries, diarrhea caused by Campylobacter jejuni is second only to Escherichia coli and Shigella. In recent years, with the enhancement of the drug resistance of Campylobacter jejuni, the degree of contamination in food has increased, and the food poisoning incidents caused by it have increased rapidly. The World Health Organization (WTO) has listed the disease as one of the most common infectious diseases, and my country's National Foodborne Disease Surveillance Network has increased the monitoring of campylobacteriosi...

AI Technical Summary

Problems solved by technology

Since the above-mentioned drug sensitivity test is based on culture, it takes a long time, about 2-3 days to get the result, and the sensitivity is poor. Therefore, there is an urgent need for rapid and sensitive experimental methods to detect bacterial drug resistance and guide clinical practice. Treatment options to control the spread and spread of drug-resistant bacteria

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