Multiple PCR method for detecting quinolone antibiotics campylobacter jejuni and kit

A technology of Campylobacter jejuni and quinolones, applied in the biological field, can solve the problems of long time consumption and poor sensitivity, and achieve the effects of fast and accurate detection, good sensitivity and simple detection technology
CN103525930BActive Publication Date: 2015-06-17CHINA AGRI UNIV
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2015-06-17

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention integrates mispairing amplification mutation analysis PCR (MAMA-PCR) and a multiple PCR technique, and provides a method for rapidly detecting quinolone antibiotics campylobacter jejuni caused by point mutation of nucleotide and a kit. The kit comprises a primer composition with the nucleotide sequence shown by SEQ ID No.1-5 and SEQ ID No.7 in the sequence list and a quality control group primer composition with the nucleotide sequence shown by SEQ ID No.1-6 in the sequence list. By adopting the detection method and the kit, campylobacter jejuni of the quinolone antibiotics can be rapidly identified from suspected bacteria, and complex procedures of the traditional detection method using strain identification and drug sensitive test as the representative are simplified. The method and the kit have favorable specificity and sensitivity, and are very suitable for rapidly detecting suspected bacteria at the frontline to provide accurate medicine instructions.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular biology technique for rapidly detecting quinolone-resistant antibiotic Campylobacter jejuni. Background technique

[0002] Campylobacter jejuni is a major food-borne infectious pathogen, and diarrhea caused by Campylobacter jejuni infection ranks first among bacterial diarrheal diseases in many economically developed countries. In developing countries, diarrhea caused by Campylobacter jejuni is second only to Escherichia coli and Shigella. In recent years, with the enhancement of the drug resistance of Campylobacter jejuni, the degree of contamination in food has increased, and the food poisoning incidents caused by it have increased rapidly. The World Health Organization (WTO) has listed the disease as one of the most common infectious diseases, and my country's National Foodborne Disease Surveillance Network has increased the monitoring of campylobacteriosi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More