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Campylobacter coli is a Gram-negative, microaerophilic, non-endospore-forming, S-shaped bacterial species within genus Campylobacter, that appears in most stool sample with diarrhea. But that does not exclude some strains of E. coli that are known to be harmless and residents of the human gut.
SgRNA, rpa primer pair and application thereof in detection of campylobacter coli
This invention discloses an sgRNA and RPA primer pair and their application in the detection of Campylobacter coli, wherein the sgRNA contains a target sequence with a nucleotide sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The detection method of this invention has a limit of detection of 10. 0 With a concentration of CFU / mL, it exhibits high specificity, simple detection operation, and is not limited by site, equipment, or operator expertise. It has a wide range of applications and can be used for rapid detection of Campylobacter coli in actual fecal samples, with accurate results.
Owner:YANGZHOU UNIV