5 results about "Campylobacter coli" patented technology

The invention discloses a campylobacter coli bacteriophage and application thereof, and relates to the technical field of biology. The campylobacter coli bacteriophage is preserved in the China Center for Type Culture Collection on September 25, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO.M 20242066. The invention further discloses a preparation method of the campylobacter coli bacteriophage. The campylobacter coli bacteriophage is separated from an excrement sample of a poultry farm in Hubei province, and basic characterization and application effect evaluation are carried out on the bacteriophage through whole genome sequencing, electron microscope observation, biological characteristic experiments and the like. Results show that the bacteriophage not only is fast in replication and easy to amplify, but also has good stable activity. The bacteriophage has a wide lysis spectrum, can effectively inhibit proliferation of campylobacter, can be developed into antibacterial products, and has important market application value.

This invention discloses an sgRNA and RPA primer pair and their application in the detection of Campylobacter coli, wherein the sgRNA contains a target sequence with a nucleotide sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The detection method of this invention has a limit of detection of 10. 0 With a concentration of CFU / mL, it exhibits high specificity, simple detection operation, and is not limited by site, equipment, or operator expertise. It has a wide range of applications and can be used for rapid detection of Campylobacter coli in actual fecal samples, with accurate results.

The invention discloses a primer composition for detecting pathogenic campylobacter based on a loop-mediated isothermal amplification technology and a detection method, and belongs to the technical field of biological detection of pathogenic bacteria. The detection method provided by the invention can quickly and accurately detect whether a sample to be detected contains pathogenic campylobacter jejuni and campylobacter colons or not, specifically, detection can be completed within 20 minutes, a detection result can be read, and the detection time is obviously shorter than that of a real-time fluorescent quantitative PCR method which is commonly adopted at present; the method has important application value in public health, food safety, animal husbandry and veterinary medicine and entry and exit inspection and quarantine.

The present invention discloses a primer combination and detection method for detecting pathogenic Campylobacter based on loop-mediated isothermal amplification technology, belonging to the field of biological detection technology for pathogenic bacteria. The detection method provided by the present invention can quickly and accurately detect whether a sample contains pathogenic Campylobacter jejuni and Campylobacter coli. Specifically, the detection and reading of the test results can be completed within 20 minutes, which is significantly shorter than the currently commonly used real-time fluorescence quantitative PCR method. It has important application value in public health, food safety, animal husbandry and veterinary medicine, and entry-exit inspection and quarantine.

Disclosed are nucleic acid oligomers, including amplification oligomers, detection probes, and combinations thereof, for detection of one or more gastrointestinal pathogens selected from Salmonella, Shigella, Campylobacter jejuni, and Campylobacter coli. Also disclosed are methods of specific nucleic acid amplification and detection, including multiplex assays, using the disclosed oligomers, as well as corresponding reaction mixtures and kits.