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Primer and method for fast typing campylobacter jejuni

A rapid technology for Campylobacter jejuni, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve good specificity, simple and fast operation, and auxiliary clinical diagnosis effects

Active Publication Date: 2016-08-24
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no report on the application of AILP technology to the genotyping of Campylobacter jejuni

Method used

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  • Primer and method for fast typing campylobacter jejuni
  • Primer and method for fast typing campylobacter jejuni
  • Primer and method for fast typing campylobacter jejuni

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, primer

[0025] The primer provided by the invention is used for rapid typing of Campylobacter jejuni.

[0026] Table 1 Primers provided by the present invention

[0027]

[0028]

Embodiment 2

[0029] Example 2, the length of each target gene interval segment of Campylobacter jejuni

[0030] The length of the mnmA target intergenic fragment provided by the present invention is 201bp, the length of the trpC target intergenic fragment is 253bp, the length of the rpmH target intergenic fragment is 315bp, the length of the acs target intergenic fragment is 436bp, and the length of the ansA target intergenic fragment is The length of the ppk target intergenic fragment is 3170bp, the length of the metB target intergenic fragment is 637bp, the length of the surE target intergenic fragment is 1270bp, the length of the rplT target intergenic fragment is 823bp, and the length of the dnaB target intergenic fragment is 500bp. The length of the target intergenic fragment of arsR is 923bp, the length of the target intergenic fragment of arsR is 1176bp, the length of the target intergenic fragment of ksgA is 2545bp, and the length of the target intergenic fragment of tonB is 1763bp....

Embodiment 3

[0034] Embodiment 3, a kind of method for rapid typing of Campylobacter jejuni

[0035] 1. Extraction of Genomic DNA of Campylobacter jejuni:

[0036] Take the 36 strains of Campylobacter jejuni to be tested, respectively densely streak on the blood agar plate medium, place in 5% O 2 , 10% CO 2 and 85%N 2 Under microaerophilic conditions, after 24-48 hours of enrichment culture at 37°C, scrape all the colonies on the medium to 2ml ddH 2 In O, vortex and oscillate to mix and then centrifuge at 10000rpm for 2min, discard the supernatant, and operate according to the bacterial genomic DNA small-scale purification kit of Takara Company, extract 36 strains of Campylobacter jejuni genomic DNA respectively, and extract the DNA The concentration is 10-200ng / μl. The obtained genomic DNA was stored at -20°C before testing.

[0037] 2. PCR amplification of different gene interval segments of the strain to be tested:

[0038] With 13 pairs of Campylobacter jejuni primers provided in...

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Abstract

The invention belongs to the field of microorganism molecular typing and particularly relates to a primer and a method for fast typing campylobacter jejuni. The primer includes 13 pairs of campylobacter jejuni primers. The primer is good in typing capacity, high in distinguishing index and capable of accurately typing the campylobacter jejuni. In addition, the method for fast typing the campylobacter coli is simple and fast to operate, good in stability and repeatability, capable of effectively assisting the clinical diagnosis of diseases infected by the campylobacter jejuni and ideal.

Description

technical field [0001] The invention belongs to the field of microbial molecular typing, and in particular relates to a primer and a method for rapid typing of Campylobacter jejuni. Background technique [0002] Campylobacter is an important class of food-borne zoonotic pathogens that can cause gastrointestinal infections in humans and animals. As early as 1980, the World Health Organization listed Campylobacter enteritis as one of the most common intestinal infectious diseases Among them, Campylobacter jejuni is the most common species in Campylobacter infection, accounting for about 80-90%. [0003] In recent years, the infection rate of Campylobacter jejuni has generally been on the rise all over the world. In developed countries in Europe and the United States, the infection rate is 50-100 / 100,000, and the number of diarrhea cases caused even exceeds that of Salmonella and Shigella, ranking first. In addition, Campylobacter jejuni can also cause diseases such as Guilla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689C12Q2600/156
Inventor 毕水莲罗永文
Owner GUANGDONG PHARMA UNIV
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