Construction method of date tree SSR (Simple Sequence repeat) marker molecular genetic map

A technology for labeling molecules and genetic maps, which is used in biochemical equipment and methods, and the determination/inspection of microorganisms to reduce experimental costs, improve efficiency and accuracy, and improve utilization efficiency.

Inactive Publication Date: 2013-06-26
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

However, these markers are gradually less used in the construction of genetic maps

Method used

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  • Construction method of date tree SSR (Simple Sequence repeat) marker molecular genetic map
  • Construction method of date tree SSR (Simple Sequence repeat) marker molecular genetic map
  • Construction method of date tree SSR (Simple Sequence repeat) marker molecular genetic map

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Embodiment Construction

[0039] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, specific embodiments will be described in detail below. Unless otherwise specified, the technical means used in the examples are conventional means well known to those of ordinary skill in the art, and the raw materials used are all commercially available items.

[0040] The construction method of the jujube tree SSR marker molecular genetic map that the embodiment of the present invention provides comprises:

[0041] Step 1 DNA extraction

[0042] The genomic DNA extraction method of the jujube tree adopts the improved CTAB method, and the concrete steps are as follows:

[0043] Step 11 Take an appropriate amount of CTAB extract and put it in a 65°C water bath to preheat for use;

[0044] Step 12 Take 0.5 g of jujube leaves, put them into a mortar, add a small amount of PVP-40, grind them into powder quickly in liquid nitrogen, transfer to 1ml of...

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Abstract

The invention discloses a construction method of a date tree SSR (Simple Sequence repeat) marker molecular genetic map. The construction method comprises the following steps of: (1) extracting genomic DNAs (deoxyribonucleic acids) of parents and offsprings of a date tree to be detected; (2) screening SSR primers by using parent materials; (3) carrying out PCR (Polymerase Chain Reaction) amplification by respectively using the SSR primers and respectively taking the genomic DNAs (deoxyribonucleic acids) of the parents and offsprings of the date tree to be detected as templates; and (4) analyzing PCR amplification results by using biology software, and constructing the date tree SSR marker molecular genetic map. According to the construction method, the high-efficiency low-cost date tree SSR marker technical system is constructed, and obtained SSR markers and a core primer group have the advantages of high efficiency, stability, co-dominance and the like. The set of markers can be used for researches such as the date tree genetic map construction, genetic diversity analysis, identification of variety, molecular mark assisted breeding and the like and promoting the development of date tree genetics and breeding technique.

Description

technical field [0001] The invention relates to the fields of molecular biology, genetics and genomics, in particular to a method for constructing a jujube tree SSR marker molecular genetic map. Background technique [0002] Jujube belongs to the genus Jujube in Rhamnaceae and is a unique fruit tree native to my country. It has extremely rich germplasm resources and complex genetic background. So far, it has a cultivation history of at least 7,000 years. In the process of long-term natural evolution and artificial selection, rich variations have been formed. So far, more than 880 varieties and excellent types of jujube trees have been discovered and recorded. However, due to the lack of a large number of polymorphic molecular markers, the research on its genetics and genomics is relatively lagging behind. [0003] Shen Lianying constructed the first jujube molecular genetic map (Shen Lianying. 2005. Construction of genetic linkage map of jujube (Ziziphus jujuba Mill) and QT...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 庞晓明王斯琪李颖岳刘君续九如
Owner BEIJING FORESTRY UNIVERSITY
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