EST-SSR molecular identification method for E.wushanense and easily mixed species thereof

A technology of molecular identification and Epimedium, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as differences in the composition and content of medicinal ingredients, and achieve rapid and accurate identification, expansion Stable results and strong specificity
CN110205397AActive Publication Date: 2019-09-06WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
Publication Date
2019-09-06

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Abstract

The invention discloses an EST-SSR molecular identification method for E.wushanense and an easily mixed species thereof, and belongs to molecular markers and medicinal material identification in the technical field of molecular biology. An EST-SSR primer group for identification of E.wushanense and the easily mixed species thereof include 10 pairs of EST-SSR core primers and common M13 primers having the sequences of SEQ ID NO:1, 2...20 and 21. The identification method includes the steps: (1) extracting total DNA of a sample by a CTAB method; (2) amplifying by PCR by using total DNA of the sample to be determined as a template; (3) analyzing a product of PCR amplification; and (4) constructing a phylogenetic tree. An application comprises identification of E.wushanense and the easily mixed species thereof. The 10 SSR molecular markers provided by the invention have good polymorphism, high specificity and stable amplification results, can rapidly and accurately identify E.wushanense and the easily mixed species thereof, provide technical support for promotion of the quality standard of epimedium medicinal materials, and are beneficial to development and utilization of the epimediummedicinal materials.
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Description

technical field

[0001] The invention belongs to molecular markers and identification of medicinal materials in the technical field of molecular biology, and specifically relates to a kind of Epimedium wushanense ( E. wushanense ) and its easily mixed species Epimedium jinchengshan ( E. jinchengshanense ), Zhenping Epimedium ( E. ilicifolium ), Epimedium pseudo-Wusshanense ( E. pseudowishanense ), Qianbei Epimedium ( E. borealiguizhouense ) EST-SSR molecular identification method. Background technique

[0002] Epimedium, a traditional Chinese medicinal herb, is the dried leaf of a plant of the genus Epimedium in the family Berberidaceae. There are about 62 known species of Epimedium in the world, and about 52 species in my country (Stearn, WT. The Genus Epimedium and Other Herbaceous Berberidaceae. Portland: Timber Press, 2002.; Ying, TS., Boufford, DE., Brach , AR. Epimedium L. St. Louis:Science Press, MissouriBotanical Garden Press, 2011, 787-799.), each edition o...

Claims

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