Method for fast detecting genome DNA residues in total RNA sample

A sample and genome technology, applied in the biological field, can solve the problems of genomic DNA contamination, whether the DNA has been removed completely cannot be known, and the quality of cDNA cannot be predicted.
CN108754010AActive Publication Date: 2018-11-06INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
3 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Publication Date
2018-11-06

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a method for fast detecting genome DNA residues in a total RNA sample. The method comprises the steps that 1, according to genetic information of a to-be-detected biological material, a gene containing one or more intron sequence is selected as a target, and for the intron sequence, PCR primers are designed; 2, the total RNA of the to-be-detected biological material is extracted, the total RNA or cDNA obtained through reverse transcription of the total RNA is used as a template, and the primers in the step 1 are used for PCR amplification; 3, a PCR amplification productis analyzed. According to the method, only one pair of the primers are designed, so that whether or not the genome DNA residues exist in the total RNA and cDNA samples of the biological material can be detected simply, conveniently and fast; the method provides support for the accuracy of gene expression analysis and has important scientific significance for promoting fundamental research of the molecular biology.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for rapidly detecting genomic DNA residues in total RNA samples. Background technique

[0002] Lily (Lilium spp.) is a multi-purpose plant with a long history of cultivation in my country for medicine, food and appreciation, and one of the top five fresh cut flowers in the world. With the continuous development of modern molecular biology, lily color (Yamagishi M, Yoshida Y, NakayamaM. The transcription factor LhMYB12 determines anthocyanin pigmentation in thetepals of Asiatic hybrid lilies (Lilium spp.) and regulates pigmentquantity. Molecular Breeding, 2012, 30 (2 ):913-925.), Huaxiang (Du F, Fan JM, Wang T, etal. Identification of differentially expressed genes in flower, leaf and bulbscale of Lilium oriental hybrid 'Sorbonne' and putative control network forscent genes. BMC Genomics, 2017 , 18(1):899.), development (Yang PP, Xu LF, Xu H, etal. Histological and transcrip...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More