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Talaromyces purpureogenus strain, biocontrol agent and application of Talaromyces purpureogenus strain

A technology of biocontrol agent and cyanobacteria, which is applied in the fields of application, biocide, fungicide, etc., can solve the problems such as difficulties in the control of wheat head blight, achieve the effect of efficiently inhibiting the germination of Fusarium graminearum spores, and have a good application prospect

Active Publication Date: 2021-10-22
ZHEJIANG UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The present invention provides a strain of Talaromyces purpureogenus TP18, a biocontrol agent and its application to solve the problem of difficult control of wheat scab

Method used

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  • Talaromyces purpureogenus strain, biocontrol agent and application of Talaromyces purpureogenus strain
  • Talaromyces purpureogenus strain, biocontrol agent and application of Talaromyces purpureogenus strain
  • Talaromyces purpureogenus strain, biocontrol agent and application of Talaromyces purpureogenus strain

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Embodiment 1

[0039] Isolation, purification and screening of embodiment 1 bacterial strain

[0040] Weigh 1g of the field soil of Zhejiang Academy of Agricultural Sciences and add it to a plastic test tube filled with 10mL 0.7mol / L NaCl medium, shake and mix, and dilute the mixed liquid step by step to 10-1, 10-2, 10-3 , take 100uL of the liquid after mixing, spread it on the PDA added with antibiotics, repeat each gradient three times, culture it upside down at 25°C, observe the plate every day, purify the strains grown on the plate, and purify the purified strains Carry out confrontation culture with Fusarium graminearum PH-1, the specific steps are: use a hole puncher with an inner diameter of 6 mm to punch holes in the outer circle of the activated strain, and inoculate to one side of a PDA plate with a diameter of 9 cm, and place a hole on the other side of the plate. One side was inoculated with a 6 mm diameter plate of Fusarium graminearum PH-1. The distance between the two bacteri...

Embodiment 2

[0041] Morphological observation and identification of embodiment 2 bacterial strain TP18

[0042] The strain was inoculated on a PDA plate and cultured at 25°C for 7 days, the colony was blue in the middle and yellow in the edge, producing a large number of conidia, see figure 1 .

[0043] The DNA of the strain was extracted and amplified by fungal universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') (SEQ ID NO: 1) and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') (SEQ ID NO: 2). The PCR reaction system was 25 μL: 1 μL of upstream and downstream primers (10 μmol / L) ITS1 and ITS4, 2.5 μL of DNA template, 12.5 μL of mix, and 8 μL of ddH2O. 94°C for 5min; 94°C for 30s, 53°C for 1min, 72°C for 1min, 30 cycles; 72°C for 10min. The PCR amplification product was sent to Hangzhou Qingke Biotechnology Co., Ltd. for sequence analysis. The sequencing results are as follows:

[0044] GAGGCCCGGTGGAGGGGGGGCGCGAGGGGGCCTCACTCGGTAATGATTCCTCCGCCTTATTGATATGCTTAAGTTCAGCGGGTAACTCCTACCTGATCCGAGGTCAACCTTGTATTAA...

Embodiment 3

[0046] Embodiment 3 Cyanobacterium TP18 and Fusarium graminearum plate confrontation test

[0047] Use a hole puncher with an inner diameter of 6mm to punch holes in the outer ring of Talaromyces purpureogenus TP18, and inoculate it on one side of a 9cm PDA plate, and inoculate a 6mm diameter of Sisarium graminearum on the other side of the plate. Bacteria PH-1 dish. The interval between cyanobacteria and Fusarium graminearum is about 6cm. One side of the PDA plate was inoculated with Fusarium graminearum PH-1 and the other side was not treated as a negative control, and repeated 3 times. The strain was cultured in an incubator at 25°C with a photoperiod of 12 hours. After 7 days, the width of the inhibition zone was observed, and the inhibition rate of the cyanobacteria to the mycelial growth of Fusarium graminearum PH-1 was calculated. Mycelia growth inhibition rate (%)=(control group PH-1 colony diameter-treatment group PH-1 colony diameter) / control group PH-1 colony diam...

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Abstract

The invention discloses a Talaromyces purpureogenus strain, a biocontrol agent and application of the Talaromyces purpureogenus strain. The classification name of the Talaromyces purpureogenus strain is Talaromyces purpureogenus TP18, and the accession number of the Talaromyces purpureogenus strain is CGMCC No. 22403. The strain has a good antagonistic effect on fusarium graminearum, and can be applied to prevention and treatment of wheat scab; and the biocontrol agent prepared from the fermentation broth of the strain has a strong inhibition effect on spore germination, hypha growth and vomitoxin yield of Fusarium graminearum, and has good application prospects.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, in particular to blue-shaped bacterial strains, biocontrol agents and applications. Background technique [0002] Wheat head blight, also known as wheat ear blight, is one of the main diseases of wheat. In my country, wheat scab is mainly caused by Fusarium graminearum and Fusarium asiatica. After wheat is infected by pathogens, it will also produce a variety of mycotoxins, such as deoxynivalene and zearalenone, etc. These mycotoxins have teratogenic and carcinogenic effects and can damage the immune system of humans and animals, thereby Serious threat to human and animal health. [0003] At present, the prevention and control of wheat head blight mainly depends on four aspects: cultivating disease-resistant varieties, strengthening field management, chemical control and biological control. Among them, the biggest problem facing the breeding of scab-resistant wheat is the la...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30
Inventor 尹燕妮王宁商庆华陈云马忠华
Owner ZHEJIANG UNIV
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