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Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection

A technology of Trichoderma harzianum and compound bacteria, applied in the biological field, can solve the problems of short validity period, poor colonization ability, limited application scope, etc., and achieve the effects of plant growth promotion, stable and long-lasting effect, and small environmental impact.

Inactive Publication Date: 2009-11-11
刘和现
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, most of the biocontrol bacteria for plant diseases are fungi and bacteria isolated from soil, rhizosphere or plant surface, but the ecological environment in which they live is affected by harsh environmental factors such as ultraviolet rays, storms and chemicals, resulting in Its colonization ability is poor, and it often has a certain control effect in the laboratory, but in the field production practice, the control effect is often unstable.
In addition, because biological control bacteria are generally sensitive to chemical fungicides and have a short validity period, it is difficult to prevent and control most diseases, which seriously limits their application range.

Method used

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  • Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection
  • Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection
  • Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection

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Embodiment 1

[0084] The control experiment of the compound microbial agent of the present invention on the prevention and treatment of cucumber powdery mildew and the promotion of cucumber yield increase.

[0085]Three control products, MDCGTH 18, carbendazim, and fenxining, were used to treat the seeds and plants in the same way, and then untreated seeds and plants were used as a control experiment. The treatment method is as follows: soak the seeds with 4% concentration of bacterial agent for 5 hours; use 2% concentration of bacterial agent to spray the seedbed twice at the seedling stage, once every three days; spray the leaves and flowers three times during the growth period, once every five days; Spray 3 times during the fruiting period, once every five days; spray 3 times on the fruit leaves during the fruiting period, once every seven days. On the 3rd day of cucumber seedlings, 20 strains of each group were selected to be inoculated with powdery mildew bacteria, and the number of in...

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Abstract

The invention relates to a trichoderma harzianum composite bacteria culture and application of the same in the aspect of plant protection, and belongs to the technical field of biology. The strain of the trichoderma harzianum composite bacteria culture is preserved in the China General Microbiological Culture Collection Center (CGMCC), and classified and named as Trichoderma harzianum MDCGTH 18, with a preservation number of CGMCC NO.2965. The Trichoderma harzianum MDCGTH 18 composite bacteria culture utilizes antagonistic microbes for biological prevention and treatment, simultaneously has the functions of inducing the disease resistance of plants and increasing the yield, has obvious effect of controlling aspen root rot, apple root rot, citrus yellow shoot, watermelon fusarium wilt, tobacco bacterial wilt, wheat scab, full rot, root rot, banded sclerotial blight, cucumber powdery mildew, downy mildew, vegetable gray mold, fusarium wilt and various other epidemic diseases, can be independently taken as a biological control microbial inoculum for plant diseases, and can also be mixed with other biological antiseptics, plant antibiotics, plant growth regulators and the prior antiseptics to prepare various biological antiseptics for controlling different plant diseases.

Description

Technical field: [0001] The invention relates to a screening and cultivating method of a compound bacterium of Trichoderma harzianum and Chaetomium and its application in biological control bacteria agents, belonging to the field of biotechnology. technical background: [0002] Worldwide, plant diseases commonly cause severe losses to agricultural production. Production is mainly based on chemical control, which not only makes it difficult for the medicine to be effective for a long time, but also seriously pollutes the environment. The application of pesticides to crops will greatly affect the quality, and seriously endanger human health, leading to human and animal poisoning and other consequences. With the strengthening of human awareness of environmental protection, vigorously developing green agriculture has become a requirement of the new era. As a content of green agriculture, biological control has attracted more and more attention. In the field of biological contr...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/04A01N63/04A01N63/02A01N43/16A01N33/22A01N47/36A01P3/00C12R1/885C12R1/645
Inventor 刘和现刘冰刘旭
Owner 刘和现
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