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Preparation and applications of Paenibacillus polymyxa GY40 and wettable powder of Paenibacillus polymyxa GY40

A technology of polymyxa spore-like and wettable powder, which is applied to the field of fermentation and culture of Paenibacillus polymyxa GY40 strain, Paenibacillus polymyxa GY40 strain, wettable powder, and preparation of bacterial powder, and can solve the problem of high culture temperature and adaptability to Problems such as poor performance and few excellent strains in production

Active Publication Date: 2018-07-13
JIANGSU GENGYUN CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is aimed at the deficiencies of the prior art, especially the deficiencies such as fewer excellent bacterial strains in production, poor adaptability, and higher cultivation temperature, and provides a salt-tolerant, low-cultivation temperature and simple nutrition derived from the ocean. A New Paenibacillus polymyxa GY40 Strain CGMCC NO.14481

Method used

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  • Preparation and applications of Paenibacillus polymyxa GY40 and wettable powder of Paenibacillus polymyxa GY40
  • Preparation and applications of Paenibacillus polymyxa GY40 and wettable powder of Paenibacillus polymyxa GY40
  • Preparation and applications of Paenibacillus polymyxa GY40 and wettable powder of Paenibacillus polymyxa GY40

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Embodiment 1

[0313] The specific technical solutions of the present invention are further described below, so that those skilled in the art can further understand the present invention, without limiting their rights. Example 1, a strain of Paenibacillus polymyxa GY40, its preservation number is: CGMCC NO.14481.

[0314] The spore-producing fermentation culture method of the described Paenibacillus polymyxa GY40 bacterial strain, its steps are as follows:

[0315] (1) Inoculate the GY40 strain on the PDA medium and activate it for 24 hours. Under sterile conditions, pick the GY40 strain with an inoculation loop and place it in a 250mL Erlenmeyer flask containing 60mL of seed liquid medium, and cultivate it at 28°C for 16h with shaking at 180r / min. ; Adjust the bacterial concentration to 10 9 cells / mL, as seed liquid for fermentation; the formula of the seed liquid culture medium is: glucose 20g / L, potato 200g / L, water 1L;

[0316](2) The seed solution is inoculated into a fermenter equipp...

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Abstract

The invention discloses a Paenibacillus polymyxa GY40 bacterial strain. The preservation number is CGMCC NO.14481. The invention also discloses a spore production fermentation culture method of the Paenibacillus polymyxa GY40 bacterial strain, and a method used for preparing bacterial powder and a wettable powder from the Paenibacillus polymyxa GY40 bacterial strain. Compared with terrestrial microorganisms, the Paenibacillus polymyxa GY40 bacterial strain possesses following advantages: salt resistance is excellent, growth temperature is low, and antibacterial effect is excellent. The spore production fermentation culture method is simple in formula; the main raw materials are agricultural product leftovers; the cost is low; the raw materials are widely available; bacterial strain growthspeed is high; fermentation temperature is low; fermentation time is short; fermentation conditions are convenient to control; fermentation spore yield is high; raw material cost is reduced; fermentation energy consumption is reduced; fermentation production efficiency is increased; the wettable powder possesses excellent preventing and treating effect on wheat scab and fusarium wilt of cucumber,and can be used in plantation of wheat and cucumber.

Description

technical field [0001] The present invention relates to a bacterial strain derived from the ocean, in particular to a Paenibacillus polymyxa GY40 bacterial strain, and the present invention also relates to a fermentation and cultivation method of the Paenibacillus polymyxa GY40 bacterial strain, using the bacterial strain to prepare bacteria powder and wettable powder methods and uses. Background technique [0002] Bacillus can produce endophytic spores with strong stress resistance, and the spores are suitable for storage and transportation, so they are the main active ingredients of the developed microbial agents. At present, most of the successfully developed biocontrol live bacteria preparations use spores as the main component. To study the spore-producing medium and fermentation conditions of the strains, and to improve the fermentation level and spore yield is the key to the development of live bacillus preparations, which is of great significance for improving produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01N25/14A01P3/00C12R1/12
CPCA01N25/14A01N63/00A01N63/10C12N1/20C12N1/205C12R2001/12
Inventor 李楠贾杰张海军刘慕兰李惠陶忠蒋涛丁正庭徐全德
Owner JIANGSU GENGYUN CHEM CO LTD
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