KASP primer group for detecting gibberellic disease resistance of Yangmai series wheat and application thereof

A technology of wheat head blight and primer set, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., to achieve intuitive results and improve effectiveness

Active Publication Date: 2021-04-30
JIANGSU LIXIAHE REGION AGRI RES INST
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  • KASP primer group for detecting gibberellic disease resistance of Yangmai series wheat and application thereof
  • KASP primer group for detecting gibberellic disease resistance of Yangmai series wheat and application thereof
  • KASP primer group for detecting gibberellic disease resistance of Yangmai series wheat and application thereof

Examples

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Embodiment 1

[0027] Example 1 Screening the SNP loci related to the QTL of resistance to scab in Yangmai series varieties (lines) and designing specific primers

[0028] In this example, two wheat parents Yangmai No. 4 and Yanzhan No. 1 with obvious differences in scab resistance were used to cross each other to produce F1, and 151 RIL populations were established for QTL through the addition of single-seed method and 8 generations of self-crossing. position. In 2017, 2018, and 2019, the RIL population was planted in the Wantou Experimental Base of the Institute of Agricultural Sciences in Lixiahe, Jiangsu, respectively, and in 2018, 2019, and 2020, respectively, referring to the "People's Republic of China Agricultural Industry Standard NY / T 2954-2016: Technical Regulations for Identification of Wheat Regional Test Varieties Resistance to Fusarium Fusarium, which identified the resistance of RIL populations to head blight. DNA was extracted using the Plant Genomic DNA Extraction Kit (Tia...

Embodiment 2

[0045] Embodiment 2 field experiment

[0046] Further use the traditional inoculation identification of wheat scab resistance in the field to prepare Gibberella spore suspension (1 × 10 5 —5×10 5 Spores mL–1) were inoculated by single-flower dripping method. At the early flowering stage of wheat, 10 μL of spore liquid was drawn with a syringe and injected into any floret of the wheat ear from the top spikelet to the sixth spikelet from top to bottom. Varieties were inoculated with 30 ears. After inoculation, use artificial mist to moisturize (spray mist for 5 minutes every half hour). 21 days after inoculation, the number of diseased spikelets in the inoculated ears was investigated, and the rate of diseased spikelets was calculated. Rate of diseased spikelets = number of diseased spikelets / total number of spikelets × 100%. The disease resistance investigation and identification standards are in accordance with the "Agricultural Industry Standard of the People's Republic o...

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Abstract

The invention provides a KASP primer group for detecting gibberellic disease resistance of Yangmai series wheat varieties (lines) and an application of the KASP primer group. The KASP primer group comprises a primer 1 with a nucleotide sequence shown as SEQIDNO.1, a primer 2 with a nucleotide sequence shown as SEQIDNO.2 and a primer 3 with a nucleotide sequence shown as SEQIDNO.3. The PCR amplification is carried out by taking the KASP primer group as a primer and taking a wheat genome to be detected as a template, then a PCR amplification product is detected by utilizing a multifunctional microplate reader and genotyping is carried out, and if a genotyping result shows that the genotyping of the wheat to be detected is the same as that of Yangmai No.4, the wheat to be detected contains an allele G; otherwise, the wheat to be detected contains the allele A; the gibberellic disease resistance of the wheat containing the allele G is higher than that of the wheat containing the allele A; the detection method can rapidly and visually obtain the result of the wheat variety gibberellic disease resistance genotype, and is suitable for large-scale breeding screening.

Description

technical field [0001] The application relates to the field of wheat breeding, in particular to a set of KASP primers used for detecting scab resistance of Yangmai series wheat varieties (lines) and its application. Background technique [0002] The wheat region in the middle and lower reaches of the Yangtze River has always been a recurrent area of ​​wheat scab in my country, and breeding of wheat varieties resistant to scab is an important breeding goal in this wheat region. According to reports, from 2012 to 2015, the average annual occurrence area of ​​scab in Jiangsu Province was about 1.2 million hm2. 2 . The root cause of the rapid expansion and large outbreaks of scab in production is that there are no varieties that are stably and highly resistant to scab on a large scale. Therefore, when the temperature and humidity are suitable, the pathogens infect the ears of wheat in large numbers, and the wheat itself Insufficient disease resistance will lead to severe disea...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113
Inventor 胡文静高德荣张勇陆成彬张春梅刘大同刘健
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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