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Bacillus siamensis and application thereof

A Bacillus and strain technology, applied to Bacillus siamese and its application fields, can solve the problems of increased drug resistance, harmfulness, and chemical control cannot be used as the most suitable means, and achieves growth promotion, broad application prospects, and strong prevention and control. effect of effect

Active Publication Date: 2019-12-13
TOBACCO RES INST CHIN AGRI SCI ACAD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control is ideal, but many chemicals used to control plant fungal and bacterial diseases are harmful to animal and human health
At the same time, due to the residues of chemical pesticides and the enhancement of drug resistance caused by a single application, chemical control can no longer be the most suitable means. Therefore, safer and more environmentally friendly biological control agents are needed to replace these chemical agents.

Method used

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  • Bacillus siamensis and application thereof
  • Bacillus siamensis and application thereof
  • Bacillus siamensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Identification of bacterial strain LZ88

[0077] Using the bacterial strain LZ88 genomic DNA as a template, the bacterial 16S rDNA universal primers 27F (5'-AGAGTTTGATCMTGGCTCAG-3', SEQ ID NO.1) and 1492R (5'-TACGGYTACCTTGTTACGACTT-3', SEQ ID NO.2) were used to amplify 16S rDNA sequence. Amplification system and procedure: 25 μL of PCR system contains 5.0 μL of 5×PCR buffer, DNA template (100 ng μL -1 ) 1.0 μL, dNTPs (10 mM) 0.75 μL, forward primer (10 μM) 0.75 μL, reverse primer (10 μM) 0.75 μL, MgCl 2 (25mM) 2.5μL, Taq DNA polymerase (5UμL -1 Promega) 0.25μL, the rest ddH 2 O make up. The PCR amplification program was pre-denaturation at 94°C for 3min; denaturation at 94°C for 30s, annealing at 55°C for 30s, extension at 72°C for 1min30s, and 30 cycles. Finally, extend at 72°C for 10 min and store at 4°C. After the reaction, the PCR products were analyzed by agarose gel electrophoresis. The obtained fragments were subjected to sequencing analysis. T...

Embodiment 2

[0079] Example 2 Bacillus siamensis (Bacillus siamensis) LZ88 antibacterial ability assay

[0080] The LZ88 strain was isolated from the rhizosphere soil of tobacco, and its antibacterial activity against tobacco red spot was determined by the confrontation culture method. The activated pathogenic bacteria cake was obtained with a sterilized puncher, and the pathogenic bacteria cake was first inoculated on fresh PDA. In the center of the plate, the antagonistic bacteria point was placed at a distance of 2.5 cm from the bacterium cake, and the plate only inoculated with the bacterium cake was used as a control. Place them in a 28°C incubator upside down. After 5 days, the colony diameters of the treatment and control were measured, and the bacteriostatic rate was calculated. The results showed that strain LZ88 was effective against tobacco red spot (Alteraria alternata), tobacco black shank (Phytophthora parasitica var. sp.cucumebrium Owen) and peanut sickle disease (Fusarium...

Embodiment 3

[0081] The mensuration of the antibacterial activity of embodiment 3 volatile antibacterial substances

[0082]The activity of volatile substances was determined by the plate buckle method. Inoculate the cake of Alternaria Alternaria in the center of the PDA plate, and inoculate the strain LZ88 on the NA plate at the same time, buckle the mouths of the two petri dishes together, and seal them with a parafilm. Cultivate in a constant temperature incubator at 28°C. After 7 days, observe the growth of the control and treatment. When cultivating, it should be noted that the plate inoculated with pathogenic bacteria should be placed at the top, and the plate inoculated with antagonistic bacteria LZ88 should be placed at the bottom.

[0083] The experimental results show that on the plate, the volatile substances of the antagonistic strain LZ88 can effectively inhibit the growth of Alternaria tabacum. In the control, the Alternaria has covered the entire plate, and in the experimen...

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Abstract

The invention provides Bacillus siamensis and application thereof, and belongs to the technical field of microbial prevention and control. Research shows that Bacillus siamensis LZ88 provided by the invention has very good effects on the biological control of plant diseases such as tobacco brown spot, tobacco black shank, wheat scab, grape anthracnose, cucumber fusarium wilt, peanut fusarium and the like, the induction of the resistance to tobacco plant diseases and the like, and provides a new strain resource for biological prevention and control of main diseases of tobacco, such as brown spot, and the like, thereby having good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to a strain of bacillus siamese and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tobacco red spot disease is a kind of disease caused by Alternaria Alternata at the late stage of tobacco plant maturity. The disease was first discovered in the United States in 1892. Since it was reported, the harm of tobacco red spot disease has become more and more serious, and it has commonly occurred in various smoking areas in the world, and has caused serious economic losses to the world's tobacco leaf production several times. An outbreak in North ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 王晓强王凤龙任广伟沈宏雷强谢中玉孙光军李斌李光雷杨举田冯超徐传涛余佳敏
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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