Paenibacillus polymyxa and application thereof

A polymyxa spore-like and bacillus technology, applied in the field of microorganisms, can solve problems such as widespread contamination of gibberellins, and achieve the effects of being suitable for popularization and use, improving quality, and low production and use costs

Active Publication Date: 2015-09-02
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of scab disease and gibberellic toxin widespread pollution in wheat, a strain of Paenibacillus polymyxa that has a control effect on scab and

Method used

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  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening Paenibacillus polymyxa

[0026] Collect the ears of wheat in Huai'an, Jiangsu, after surface disinfection, grind the ears under aseptic conditions, add sterile water, mix well, then perform serial dilutions, spread the dilutions of different concentrations on the LB medium, and keep at 30°C After culturing for 5 days under the same conditions, pick different colonies on the plate to verify the antagonistic ability of different colonies to Fusarium, pick strains that have antagonistic ability to Gibberella, and detect the degradation effect of antagonistic bacteria on zearalenone toxin, Finally, a strain capable of inhibiting the growth of Fusarium and degrading zearalenone toxin was obtained at the same time. The applicant named it AFHXD7, added glycerol, and stored it in a -70°C refrigerator.

[0027] The main biological characteristics of the AFHXD7 strain are: rod-shaped bacteria, Gram staining positive; oxidase, VP test, arginine dihydrolase nega...

Embodiment 2

[0030] Embodiment 2 Preparation of liquid bacterial agent

[0031] (a) Activate the Paenibacillus polymyxa strain obtained in Example 1 in a petri dish, then culture it on a test tube slant (LB medium) at 30°C for 48 hours to obtain the test tube strain;

[0032] (b) pick the strains of the two-ring test tube from the inclined surface and inoculate them into a shake flask containing LB medium, and cultivate them with shaking at 30°C-37°C at 180rpm to the logarithmic growth phase to obtain the strains;

[0033] (c) Add 400L of LB medium to a 500L seed tank, 121°C, 1.1kg / cm2 20 minutes of high-pressure damp heat sterilization, after natural cooling to 33°C, 10% of the volume of the LB medium was inserted into the strains obtained in step b, at a temperature of 30-37°C, stirred and cultured until the logarithmic growth phase, and cultured The time is about 48-60h, the stirring rate is 180-240rpm, and the sterile air is passed through during the cultivation period, and the flow ra...

Embodiment 3

[0035] Example 3 Preparation of solid microbial agent

[0036] The liquid inoculum obtained in Example 2, yeast cell wall and montmorillonite were uniformly mixed in a mass ratio of 1:1:3 to obtain a solid inoculum of Paenibacillus polymyxa.

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Abstract

The invention discloses a paenibacillus polymyxa of which the preservation number is CCTCC NO.M2015059 and an application of the paenibacillus polymyxa. The bacterial strain is capable of effectively inhibiting germination of fusarium graminearum spores and growth of hypha, inhibiting generation of fusarium toxin, and degrading generated zearalanel toxin; no toxic by-product is generated in the process of solving toxin pollution; secondary pollution does not exist; and the paenibacillus polymyxa can be used for preventing wheat scab, reducing the content of wheat DON toxin and zearalenone, and degrading the zearalenone in grains or feeds.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Paenibacillus polymyxa and its application. Background technique [0002] Head blight is an important disease of various cereal crops such as wheat and corn, and it often occurs in warm and humid regions. As the degree of mechanical harvesting of wheat continues to increase, a large amount of straw is returned to the field, and wheat bran is scattered in the field, which gradually aggravates the occurrence of wheat scab, which is one of the main diseases that harm wheat. Fusarium graminearum complex is mainly caused by Fusarium graminearum complex. The disease mainly infects crops at the flowering stage and causes disease at the seed filling maturity stage. Moreover, the gibberellin toxin produced during the infection process directly remains and accumulates in the grains of cereal crops, seriously threatening the health of humans and animals, and is the core pollutant ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A23L1/015C12R1/01
Inventor 徐剑宏史建荣胡晓丹殷宪超祭芳徐圣佳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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