Tobacco-black-shank-resisting and tobacco-bacterial-wilt-resisting bio-control strain Trb3

Abstract

The invention belongs to the technical field of plant protection, and relates to a tobacco-black-shank-resisting and tobacco-bacterial-wilt-resisting bio-control strain Trb3. The bio-control strain is a bacillus subtilis. A method for preparing a microbial agent by using the strain Trb3 comprises the steps that: the strain Trb3 is subjected to oscillation culturing in an LB culture solution for 36-40h under a speed of 180rpm under temperature of 28 DEG C; the obtained material is centrifuged for 10min under a speed of 6000rpm; the material is diluted by using sterile water, such that the microbial agent is prepared, wherein a viable bacteria total concentration in the microbial agent finished product is 1*10<9>-1*10<10>u/ml. The Trb3 strain microbial agent is applied for root dipping before tobacco transplanting or for root irrigating during tobacco transplanting, and for root irrigating after tobacco transplanting and right before disease periods of tobacco black shank and tobacco bacterial wilt. In a greenhouse, a controlling effect of the Trb3 strain microbial agent against tobacco black shank reaches 72.3%, and a controlling effect of the Trb3 strain microbial agent against tobacco bacterial wilt reaches 65.7%. When the Trb3 strain microbial agent is applied in field, a controlling effect of the Trb3 strain microbial agent against black shank and tobacco bacterial wilt is 61.04%.

Description

technical field [0001] The invention belongs to the technical field of plant protection, in particular to a biocontrol bacterial strain Trb3 resistant to tobacco black shank and bacterial wilt, which is used for pollution-free prevention and treatment of tobacco soil-borne diseases. Background technique [0002] Tobacco black shank and bacterial wilt are two of the most devastating diseases of tobacco. [0003] Tobacco black shank disease, also known as tobacco blight, is called "black stem madness", "black root" and "aconitum disease" by tobacco farmers. It is caused by Phytophthora parasitica var nicotianae, and occurs to varying degrees in major tobacco-producing areas in China. In areas where the incidence is severe, the general incidence rate reaches 5% to 15%, and the severe one reaches more than 30%, which causes great losses to tobacco production. [0004] Tobacco bacterial wilt is a tobacco soil-borne disease caused by Ralstonia solanacearum. It is common in southe...

AI Technical Summary

Problems solved by technology

[0010] Since the current biological control technologies for tobacco black shank and bacterial wilt are all aimed at a single target, there is a lack of fungicides that can have good biocontrol effects on tobacco black shank and bacterial wilt at the same time. In Shandong and southern tobacco-growing areas, the occurrence is mostly mixed, resulting in poor control effects and high control costs.

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